Mammary gland secretion: hormonal coordination of endocytosis and exocytosis

Truchet, Sandrine; Ollivier-Bousquet, Michèle

doi:10.1017/s1751731109990589

Cited by 21 publications

(17 citation statements)

References 61 publications

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“…After TJs closure, the composition of the milk reflects the highly coordinated functioning of four main transcellular pathways in MECs, which operate to produce milk components from blood-borne and interstitial molecules [6,38]. Many transporters ( Fig.…”

Section: Transcellular Pathwaysmentioning

confidence: 99%

Physiology of milk secretion

Truchet

Honvo-Houeto

2017

Best Practice & Research Clinical Endocrinology & Metab

100

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Section: Transcellular Pathwaysmentioning

confidence: 99%

Physiology of milk secretion

Truchet

Honvo-Houeto

2017

Best Practice & Research Clinical Endocrinology & Metab

100

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“…Following polarity establishment, secretory function is fully achieved when the secretion pathway is activated to deliver cargo to and across the cell membrane (1). In the mammary gland, the MECs actively synthesize copious amounts of caseins and other milk constituents that are secreted into the alveolar lumen, primarily by exocytosis (68). Vesicle acidification is required for exocytosis in other specialized secretory tissues, such as the pancreas (69), intestine (70), prostate (71), brain (72), and salivary gland (73).…”

Section: Loss Of Znt2 Disrupted V-atpase Assembly In the Mammary Glanmentioning

confidence: 99%

Zinc transporter 2 interacts with vacuolar ATPase and is required for polarization, vesicle acidification, and secretion in mammary epithelial cells

Lee

Rivera

Kelleher

2017

Journal of Biological Chemistry

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An important feature of the mammary gland is its ability to undergo profound morphological, physiological, and intracellular changes to establish and maintain secretory function. During this process, key polarity proteins and receptors are recruited to the surface of mammary epithelial cells (MECs), and the vesicle transport system develops and matures. However, the intracellular mechanisms responsible for the development of secretory function in these cells are unclear. The vesicular zinc (Zn) transporter ZnT2 is critical for appropriate mammary gland architecture, and ZnT2 deletion is associated with cytoplasmic Zn accumulation, loss of secretory function and lactation failure. The underlying mechanisms are important to understand as numerous mutations and non-synonymous genetic variation in ZnT2 have been detected in women that result in severe Zn deficiency in exclusively breastfed infants. Here we found that ZnT2 deletion in lactating mice and cultured MECs resulted in Zn-mediated degradation of phosphatase and tensin homolog (PTEN), which impaired intercellular junction formation, prolactin receptor trafficking, and alveolar lumen development. Moreover, ZnT2 directly interacted with vacuolar H-ATPase (V-ATPase), and ZnT2 deletion impaired vesicle biogenesis, acidification, trafficking, and secretion. In summary, our findings indicate that ZnT2 and V-ATPase interact and that this interaction critically mediates polarity establishment, alveolar development, and secretory function in the lactating mammary gland. Our observations implicate disruption in ZnT2 function as a modifier of secretory capacity and lactation performance.

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“…The transcytotic pathway seems also involved in sorting at the trans-Golgi network and in the biogenesis of casein-containing secretory vesicles. The mammary epithelial cells thus coordinate the endo-cytosis of blood-borne molecules at their basal side and the exocytosis of products into milk at their apical side (Chanat et al, 1999;Chat et al, 2011;Honvo-Houeto et al, 2016;Truchet and Ollivier-Bousquet, 2009).…”

Section: From Exocytosis At the Plasma Membrane To Secretion In Generalmentioning

confidence: 99%

Meeting after meeting: 20 years of discoveries by the members of the Exocytosis–Endocytosis Club

Niedergang

Gasman

Vitale

et al. 2017

Biology of the Cell

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Twenty years ago, a group of French cell biologists merged two scientific clubs with the aim of bringing together researchers in the fields of Endocytosis and Exocytosis. Founded in 1997, the first annual meeting of the Exocytosis Club was held in 1998. The Endocytosis Club held quarterly meetings from its founding in 1999. The first joint annual meeting of the Exocytosis-Endocytosis Club took place in Paris in April, 2001. What started as a modest gathering of enthusiastic scientists working in the field of cell trafficking has gone from strength to strength, rapidly becoming an unmissable yearly meeting, vividly demonstrating the high quality of science performed in our community and beyond. On the occasion of the 20th meeting of our club, we want to provide historic insight into the fields of exocytosis and endocytosis, and by extension, to subcellular trafficking, highlighting how French scientists have contributed to major advances in these fields. Today, the Exocytosis-Endocytosis Club represents a vibrant and friendly community that will hold its 20th meeting at the Presqu'Ile de Giens, near Toulon in the South of France, on May 11-13, 2017.

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Mammary gland secretion: hormonal coordination of endocytosis and exocytosis

Cited by 21 publications

References 61 publications

Physiology of milk secretion

Physiology of milk secretion

Zinc transporter 2 interacts with vacuolar ATPase and is required for polarization, vesicle acidification, and secretion in mammary epithelial cells

Meeting after meeting: 20 years of discoveries by the members of the Exocytosis–Endocytosis Club

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