Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes

Gur, Yonatan; Breitbart, Haim

doi:10.1101/gad.367606

Cited by 324 publications

(277 citation statements)

References 37 publications

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“…In addition, it has been shown that proteins such as those present in the human sperm centrosome are essential for normal syngamy and early embryonic development [38]. Furthermore, it has been recently shown that mammalian sperm translate nuclear-encoded proteins by mitochondrialtype ribosomes [39]. Thus, there are several potential explanations for the proteins identified involved in RNA binding and in transcription in the present work.…”

Section: Resultsmentioning

confidence: 68%

Proteomic identification of human sperm proteins

Martínez-Heredia¹,

Estanyol²,

Ballescà³

et al. 2006

Proteomics

230

177

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Conventional 1-DE has in the past provided a wealth of information concerning the major sperm proteins. However, so far there are relatively few reports exploiting the potential of the present proteomic tools to identify and to study additional yet-unidentified important proteins present in human spermatozoa. In the present work, 2-DE of proteins extracted from human normozoospermic spermatozoa led to the resolution of over 1000 spots. Subsequent excision from the gels of 145 spots and MALDI-TOF MS analysis allowed the identification of 98 different proteins. The function of these proteins turned out to be energy production (23%), transcription, protein synthesis, transport, folding and turnover (23%), cell cycle, apoptosis and oxidative stress (10%), signal transduction (8%), cytoskeleton, flagella and cell movement (10%), cell recognition (7%), metabolism (6%) and unknown function (11%). As many as 23% of the proteins identified have not been previously described as being expressed in human spermatozoa. The present data provide an important clue towards determining the function of these proteins and opens up the possibility to perform additional experiments.

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Section: Resultsmentioning

confidence: 68%

Proteomic identification of human sperm proteins

Martínez-Heredia¹,

Estanyol²,

Ballescà³

et al. 2006

Proteomics

230

177

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“…35 The incorporation of labelled amino acids into polypeptides during sperm capacitation was completely inhibited by the mitochondrial translation inhibitor D-chloramphenicol but not by the cytoplasmic translation inhibitor cycloheximide, and inhibition of protein translation significantly reduced sperm motility, capacitation and in vitro fertilization rates. 36 Thus, to determine which proteins were translated and to examine the importance of translation during capacitation, we studied the changes in protein expression during capacitation and determined which proteins were newly translated by mitochondrial-type ribosomes using proteomic approaches. A total of 44 proteins were identified with reduced expression in chloramphenicol-treated sperm in comparison with capacitated sperm, and a total of 26 of 44 proteins were involved in some critical processes correlated to sperm-egg interaction events via bioinformatics analysis.…”

Section: Functional Proteomics Of a Mouse Model Of Normal Spermatogenmentioning

confidence: 99%

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Huang¹,

Sha²

2010

Asian J Androl

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Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function. The present article reviews the available scientific literature related to spermatogenesis. In addition, this study uses two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC)-MS to construct a series of proteome profiles describing spermatogenesis. This large-scale identification of proteins provides a rich resource for elucidating the mechanisms underlying male fertility and infertility.

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“…Mitochondrial ribosomedependent translation has been demonstrated to be necessary for Drosophila germ line formation (Amikura et al 2005), and mitochondrial ribosomes are also involved in the translation of nuclear encoded mammalian sperm proteins (Gur & Breitbart 2006). Taking into account the likely function of germ granules in RNA regulation, it will be interesting to find out if the interplay with mitochondria has a role in the germ granulemediated control of gene expression.…”

Section: Pachytene Pirnasmentioning

confidence: 99%

Chromatoid body and small RNAs in male germ cells

Meikar¹,

Ros²,

Korhonen³

et al. 2011

Reproduction

144

154

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The chromatoid body (CB) is a germ granule in the cytoplasm of postmeiotic haploid round spermatids that is loaded with RNA and RNAbinding proteins. Following the discovery of small non-coding RNA-mediated gene regulation and the identification of PIWI-interacting RNAs (piRNAs) that have crucial roles in germ line development, the function of the CB has slowly begun to be revealed. Male germ cells utilise small RNAs to control the complex and specialised process of sperm production. Several microRNAs have been identified during spermatogenesis. In addition, a high number of piRNAs are present both in embryonic and postnatal male germ cells, with their expression being impressively induced in late meiotic cells and haploid round spermatids. At postmeiotic stage of germ cell differentiation, the CB accumulates piRNAs and proteins of piRNA machinery, as well as several other proteins involved in distinct RNA regulation pathways. All existing evidence suggests a role for the CB in mRNA regulation and small RNA-mediated gene control, but the mechanisms remain uncharacterised. In this review, we summarise the current knowledge of the CB and its association with small RNA pathways.

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Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes

Cited by 324 publications

References 37 publications

Proteomic identification of human sperm proteins

Proteomic identification of human sperm proteins

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Chromatoid body and small RNAs in male germ cells

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