Mammalian phosphoinositide kinases and phosphatases

Sasaki, Takehiko; Takasuga, Shunsuke; Sasaki, Junko; Kofuji, Satoshi; Eguchi, Satoshi; Yamazaki, Masakazu; Suzuki, Akira

doi:10.1016/j.plipres.2009.06.001

Cited by 254 publications

(318 citation statements)

References 484 publications

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“…3a, 15 and 30 min). Phosphatidylinositol 3-phosphate (PtdIns(3)P) signalling has a regulatory role in the assembly of late endosomes and lysosomes, as well as in autophagic membrane processes 33,34 . The FYVE domain is a zinc-finger motif with high affinity for PtdIns(3)P, and the green fluorescent protein (GFP)-FYVE fusion protein has been widely used for probing subcellular localisation of PtdIns(3)P in living cells 35 .…”

Section: Resultsmentioning

confidence: 99%

Delivery of endosomes to lysosomes via microautophagy in the visceral endoderm of mouse embryos

et al. 2012

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The differentiation and patterning of murine early embryos are sustained by the visceral endoderm, an epithelial layer of polarised cells that has critical roles in multiple signalling pathways and nutrient uptake. Both nutritional and signalling functions rely upon the endocytosis of various molecules from the cell surface via the endocytic pathway. However, endocytic membrane dynamics in this embryonic tissue remain poorly understood. Here we show that the functions of rab7, a small GTP-binding protein regulating the late endocytic pathway, are essential for embryonic patterning during gastrulation. The endosomes of visceral endoderm cells are delivered via a unique microautophagy-like process to the apical vacuole, a large compartment exhibiting lysosomal characteristics. Loss of rab7 function results in severe inhibition of this endocytic pathway. our results indicate that the microautophagic process and flow of the endocytic membrane have essential roles in early embryonic development.

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Section: Resultsmentioning

confidence: 99%

Delivery of endosomes to lysosomes via microautophagy in the visceral endoderm of mouse embryos

et al. 2012

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“…Membrane phospholipids can directly influence the function of many channels, including HCN1, 2 and 4 (Pian et al, 2007;Suh and Hille, 2008); therefore, we first investigated whether endogenous PIP 2 modulated HCN channel gating in IGL neurons. At micromolar concentrations, wortmannin blocks type III phosphatidylinositol 4-kinase (PI4K III) (Nakanishi et al, 1995), thereby inhibiting the final step in the biosynthetic pathway for PIP 2 (Doughman et al, 2003;Balla and Balla, 2006;Sasaki et al, 2009). Brain slices were preincubated at 30°C in ACSF containing 15 M wortmannin or 0.1% DMSO (the vehicle as control) for ϳ45 min before recording.…”

Section: Depletion Of Endogenous Pip 2 Pools Inhibits Hcn3 Functionmentioning

confidence: 99%

“…These results demonstrate that both endogenous and exogenous PIP 2 enhance HCN3 channel gating. To test whether the gating effect of terminal phospholipids in the native HCN channel might be dependent on the phosphate groups on the inositol ring, we studied possible effects of PIP 2 's parent compounds: phosphatidylinositol (PI), which is nonphosphorylated, and phosphatidylinositol-4-phosphate (PIP), which is phosphorylated on the inositol ring at position 4 (Sasaki et al, 2009). We used the same approach to apply these compounds as for PIP 2 experiments (10 M in the recording pipette).…”

Section: Pip 2 Markedly Enhances Opening Of I H Channels In Igl Neuronsmentioning

confidence: 99%

PIP2-Mediated HCN3 Channel Gating Is Crucial for Rhythmic Burst Firing in Thalamic Intergeniculate Leaflet Neurons

Ying¹,

Tibbs²,

Picollo³

et al. 2011

Journal of Neuroscience

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Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels generate a pacemaking current, I h , which regulates neuronal excitability and oscillatory activity in the brain. Although all four HCN isoforms are expressed in the brain, the functional contribution of HCN3 is unknown. Using immunohistochemistry, confocal microscopy, and whole-cell patch-clamp recording techniques, we investigated HCN3 function in thalamic intergeniculate leaflet (IGL) neurons, as HCN3 is reportedly preferentially expressed in these cells. We observed that I h recorded from IGL, but not ventral geniculate nucleus, neurons in HCN2 ϩ/ϩ mice and rats activated slowly and were cAMP insensitive, which are hallmarks of HCN3 channels. We also observed strong immunolabeling for HCN3, with no

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“…Important inroads are being made by Lattice QCD calculations of form factors [19][20][21][22]. These and future results are very significant, as one will be able to test explicitly the behavior of the vector form factor with the quark masses, and in particular understand more accurately the SU(3) SB effects on f 1 , helping clarify the issues mentioned above.…”

Section: Introductionmentioning

confidence: 99%