Mammalian Pericardium‐Based Bioprosthetic Materials in Xenotransplantation and Tissue Engineering

Grebenik, Ekaterina A.; Gafarova, Elvira R.; Истранов, Л. П.; Истранова, Е. В.; Ma, Xiaowei; Xu, Jing; Guo, Weisheng; Atala, Anthony; Timashev, Peter

doi:10.1002/biot.201900334

Cited by 20 publications

(13 citation statements)

References 149 publications

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“…Evidently, the NC condensed mesenchyme and ensuing cartilage is encased by fibroblasts (Figure ) and the origin of these fibroblasts needs further investigation. To our knowledge second heart fieldderived cells do not have the capacity to differentiate into cartilage and neither do cultured pericardial cells [55]. In addition, fibroblasts or smooth muscle cells are found inside the myocardial OFT tube as an extension of the arterial walls (see Fig 3k, dashed line), the origin of which is also not clarified.…”

Section: Outflow Tract Separationmentioning

confidence: 88%

Ventricular Septation and Outflow Tract Development in Crocodilians Result in Two Aortas With Bicuspid Semilunar Valves

Poelmann¹,

Groot²,

Goerdajal³

et al. 2021

Preprint

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The outflow tract of crocodilians resembles that of birds and mammals as ventricular septation is complete. The arterial anatomy however, presents with a pulmonary trunk originating from the right ventricular cavum, and two aortae originating from either the right or left ventricular cavum. Mixing of blood in crocodilians cannot occur at ventricular level as in other reptiles, but instead takes place at aortic root level by a shunt, the Foramen of Panizza, the opening of which is guarded by two facing semilunar leaflets of both bicuspid aortic valves. Methods. Developmental stages of Alligator mississipiensis, Crocodilus niloticus and Caiman latirostris, have been studied. Results and Conclusions. The outflow tract septation complex can be divided into 2 components. The aorto-pulmonary septum divides the pulmonary trunk from both aortae, whereas the interaortic septum divides the systemic from the visceral aorta. Neural crest cells are most likely involved in the formation of both components. Remodeling of the endocardial cushions and both septa results in the formation of bicuspid valves in all three arterial trunks. The foramen of Panizza originates intracardially as a channel in the septal endocardial cushion.

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Section: Outflow Tract Separationmentioning

confidence: 88%

Ventricular Septation and Outflow Tract Development in Crocodilians Result in Two Aortas With Bicuspid Semilunar Valves

Poelmann¹,

Groot²,

Goerdajal³

et al. 2021

Preprint

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“…The fabrication of aortic valve bioprostheses requires a precise control of the structural-functional properties of the resulting product. Therefore, the decellularization procedure aiming at the elimination of the donor cell material must preserve the structural integrity of ECM with its inherent functions and cytocompatibility [ 38 ]. However, in complex 3D tissue structures, the decellularization is prohibited in dense or deep regions.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

et al. 2020

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One of the leading trends in the modern tissue engineering is the development of new effective methods of decellularization aimed at the removal of cellular components from a donor tissue, reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2)-assisted processing has been proposed to improve the outcome of decellularization, reduce contamination and time costs. The resulting products can serve as personalized tools for tissue-engineering therapy of various somatic pathologies. However, the decellularization of heterogeneous 3D structures, such as the aortic root, requires optimization of the parameters, including preconditioning medium composition, the type of co-solvent, values of pressure and temperature inside the scCO2 reactor, etc. In our work, using an ovine aortic root model, we performed a comparative analysis of the effectiveness of decellularization approaches based on various combinations of these parameters. The protocols were based on the combinations of treatments in alkaline, ethanol or detergent solutions with scCO2-assisted processing at different modes. Histological analysis demonstrated favorable effects of the preconditioning in a detergent solution. Following processing in scCO2 medium provided a high decellularization degree, reduced cytotoxicity, and increased ultimate tensile strength and Young’s modulus of the aortic valve leaflets, while the integrity of the extracellular matrix was preserved.

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“…The fabrication of aortic valve bioprostheses requires a precise control of structural-functional properties of the resulting product. Therefore, the decellularization procedure aiming at the elimination of the donor cell material must preserve the structural integrity of ECM with its inherent functions and cytocompatibility [37]. The approaches to decellularization based on the extraction in scCO 2 medium have been recently established for various tissues [38].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of supercritical CO2 extraction protocols in a model of ovine aortic root decellularization

Gafarova¹,

Grebenik²,

Lazhko³

et al. 2020

Preprint

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One of the leading trends in the modern tissue engineering is the development of new effective methods of decellularization aimed at the removal of cellular components from donor tissue reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2) extraction can significantly improve the outcome of decellularization, reduce contamination and time costs.The resulting products can serve as personalized tools for tissue-engineering therapy of various somatic pathologies. However, decellularization of complex 3D structures, such as the aortic root, requires optimization of the parameters, including preconditioning medium composition, type of co-solvent, values of pressure and temperature insight the scCO2 reactor, etc. In our work, using an ovine aortic root model, we performed a comparative analysis of the efficiency of decellularization using approaches based on various combinations of these parameters. The protocols were based on combinations of treatment in alkaline, ethanol or detergent solutions with scCO2 extraction at different modes. Based on a histological analysis, we have selected an optimal protocol for the decellularization of ovine aortic root employing preconditioning in a detergent solution. The positive effects of scCO2 on the decellularization extent, cytotoxicity and histoarchitecture of the tissue were demonstrated.

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Mammalian Pericardium‐Based Bioprosthetic Materials in Xenotransplantation and Tissue Engineering

Cited by 20 publications

References 149 publications

Ventricular Septation and Outflow Tract Development in Crocodilians Result in Two Aortas With Bicuspid Semilunar Valves

Ventricular Septation and Outflow Tract Development in Crocodilians Result in Two Aortas With Bicuspid Semilunar Valves

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

Evaluation of supercritical CO2 extraction protocols in a model of ovine aortic root decellularization

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