Mammalian nitrate biosynthesis: incorporation of 15NH3 into nitrate is enhanced by endotoxin treatment.

Abstract

determined by conversion of NO-to nitrobenzene, which was then analyzed by gas chromatography/mass spectrometry (22) on a HP5992 (Hewlett Packard, Palo Alto, CA). The gas chromatographic separation was carried out on a Durabond DB-5 fused silica capillary column (Quadrex, New Haven, CT) with a helium carrier flow rate of 1 ml/min, initial column temperature of 1100C, and temperature programming of 40C/min.

Nitric oxide and arthritis

“…This property explains the previously puzzling observation that mammals excrete more nitrate than they ingest, especially when inflammation is present (39). The short half-life of NO limits its immediate sphere of influence to autocrine and local paracrine effects; the high affinity of hemoglobin for NO imposes further limits.…”

Arginine metabolism: nitric oxide and beyond

“…Also, extensive research was conducted (e.g. [25]) to elucidate the metabolic basis for endogenous nitrate synthesis that had been discovered in humans and rats [26][27][28]. Key discoveries in 1987 included reports that arginine is the precursor for mammalian nitrite\nitrate synthesis [29] and that nitric oxide (NO) is the endothelium-derived relaxing factor [30,31].…”

Induction and potential biological relevance of a Ca2+‐independent nitric oxide synthase in the myocardium

“…Inflammatory reactions in vivo are known to cause a marked increase in the excretion of nitrate (Wagner et al, 1983;Stuehr & Marletta, 1985). Nitric oxide is rapidly oxidized in aqueous solution to nitrite (Palmer et al, 1987), which is itself oxidized in vivo to nitrate (Witter & Balish, 1979).…”