Mammalian Fertilization: From Sperm Factor to Phospholipase Cζ

Kurokawa, Manabu; Sato, Kenichi; Fissore, Rafael A.

doi:10.1016/j.biolcel.2003.11.003

Cited by 71 publications

(49 citation statements)

References 122 publications

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“…This difference was not significant as determined by Mann-Whitney rank sum test (PZ0.561). These results are consistent with the recently published experiments testing these fusion proteins in eggs activated by normal fertilization (Kurokawa et al 2004a, Mehlmann & Jaffe 2005 (Sakakibara et al 2005), the sperm-induced calcium transient (Abassi et al 2000), pronuclear migration and fusion (Moore & Kinsey 1995, Wright & Schatten 1995, as well as developmental competence (Livingston et al 1998, Sharma et al 2005. The extent to which these pathways are active in mammalian fertilization is now the subject of much investigation.…”

Section: Role Of Fyn In Mouse Egg Activationsupporting

confidence: 90%

“…For example, while mammalian eggs express Fyn, Yes, and in some cases, Src (Talmor et al 1998, Talmor-Cohen et al 2004a), the results of two different studies indicate that these kinases are not required for the unique sperm-induced calcium oscillations (Kurokawa et al 2004a, Mehlmann & Jaffe 2005, which trigger egg activation in mammals (Carroll 2001). Instead, these calcium oscillations are triggered directly by a sperm-borne phospholipase that does not require PTK regulation (Cox et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development

Meng

Luo²,

Li³

et al. 2006

Reproduction

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Fyn and other Src-family kinases play an essential role at several steps during egg activation following fertilization of externally fertilizing species, such as marine invertebrates, fish, and frogs. Recent studies demonstrate that the requirement for Src-family kinases in activation of the mammalian egg is different from lower species, and the objective of this study was to test the role of the Fyn kinase in the mouse egg activated by intracytoplasmic sperm injection (ICSI). An Src homology 2 (SH2) domain containing fusion protein was used to suppress Fyn function in the mouse zygote following ICSI. Eggs injected with the Fyn SH2 domain at an intracellular concentration of 4-8 mM exhibited reduced developmental potential with 100% of the zygotes being arrested following the first or the second cleavage. At higher concentrations, the protein blocked pronuclear congression and the zygotes remained at the pronuclear stage. The SH2 domain had no effect on sperm-induced calcium oscillations in distinct contrast to its effect on the eggs of lower species. The results indicate that the SH2 domain of Fyn kinase plays an important role in pronuclear congression as well as early cleavage events and that this effect appears not to involve disruption of calcium oscillations.

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Section: Role Of Fyn In Mouse Egg Activationsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development

Meng

Luo²,

Li³

et al. 2006

Reproduction

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“…It was subsequently shown that expression of cRNAs encoding for PLCZ1 in eggs initiated oscillations reminiscent of those induced by normal fertilization [7,8]. Additional studies demonstrated expression of PLCZ1 in the testes of other mammals and PLCZ1 cRNAs were able to induce oscillations in eggs of homologous and heterologous species including human PLCZ1 into human eggs [7,9,10]. Further, injection of sperm extracts depleted of PLCZ1 failed to initiate oscillations [7,8,11].…”

Section: Introductionmentioning

confidence: 99%

Protein phospholipase C Zeta1 expression in patients with failed ICSI but with normal sperm parameters

Lee

Arny

Grow

et al. 2014

J Assist Reprod Genet

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Purpose This study was conducted to determine if expression of the testis-specific phospholipase C Zeta1 (PLCZ1) correlated with low success or fertilization failure after ICSI in patients with normal parameters after standard semen analysis (SA). Methods Couples <43 years with one or two failed or low fertilization ICSI cycles. Standard Semen Analysis (SA) was performed to determine sperm parameters in male partners, whereas females were evaluated for antral follicle counts (AFC), day 3 FSH levels and peak Estradiol (E2) levels. The presence of PLCZ1 in sperm was ascertained using Western blotting and Immunofluorescence (IF) analysis. The ability of sperm to initiate changes in the intracellular concentrations of free calcium ([Ca 2+ ] i ), which is characteristic of mammalian sperm, was performed after injection of human sperm into mouse eggs loaded with the Ca 2+ sensitive dye fura-2 AM. Results Male partners of couples with failed or low success ICSI fertilization but with normal SA parameters showed low expression levels of PLCZ1 as determined by western blotting and reduced fluorescent signal during IF studies. In addition, fewer of these males' sperm showed PLCZ1 expression and were able to initiate robust [Ca 2+ ] i oscillations upon injection into eggs. Conclusion Our data suggest that in patients with normal SA parameters but with repeated low fertilization or outright failed fertilization results after ICSI, abnormal PLCZ1 function should be considered as the underlying mechanism responsible for the failure of fertilization.

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“…Initiation of development is triggered by a series of long-lasting oscillations of intracellular free calcium ([Ca 2C ] i ). Several pieces of evidence support the hypothesis that the sperm, upon fusion with the oocyte, delivers a sperm-specific isoform of phospholipase C (PLCZ; Saunders et al 2002, Kurokawa et al 2004, 2006, Malcuit et al 2006a. PLCZ has the ability to function at basal Ca 2C concentrations (Kouchi et al 2004) and thus, upon entering the oocyte's cytoplasm, induces hydrolysis of phosphatidylinositol-4, 5-bisphosphate (PIP 2 ), generating 1, 2-diacylglycerol (DAG), and IP 3 .…”

Section: Introductionmentioning

confidence: 99%

Activation of bovine somatic cell nuclear transfer embryos by PLCZ cRNA injection

Ross¹,

Rodríguez²,

Iager³

et al. 2009

Reproduction

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The production of cloned animals by the transfer of a differentiated somatic cell into an enucleated oocyte circumvents fertilization. During fertilization, the sperm delivers a sperm-specific phospholipase C (PLCZ) that is responsible for triggering Ca 2C oscillations and oocyte activation. During bovine somatic cell nuclear transfer (SCNT), oocyte activation is artificially achieved by combined chemical treatments that induce a monotonic rise in intracellular Ca 2C and inhibit either phosphorylation or protein synthesis. In this study, we tested the hypothesis that activation of bovine nuclear transfer embryos by PLCZ improves nuclear reprogramming. Injection of PLCZ cRNA into bovine SCNTunits induced Ca 2C oscillations similar to those observed after fertilization and supported high rates of blastocyst development similar to that seen in embryos produced by IVF. Furthermore, gene expression analysis at the eight-cell and blastocyst stages revealed a similar expression pattern for a number of genes in both groups of embryos. Lastly, levels of trimethylated lysine 27 at histone H3 in blastocysts were higher in bovine nuclear transfer embryos activated using cycloheximide and 6-dimethylaminopurine (DMAP) than in those activated using PLCZ or derived from IVF. These results demonstrate that exogenous PLCZ can be used to activate bovine SCNT-derived embryos and support the hypothesis that a fertilization-like activation response can enhance some aspects of nuclear reprogramming.

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Mammalian Fertilization: From Sperm Factor to Phospholipase Cζ

Cited by 71 publications

References 122 publications

Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development

Role of Src homology 2 domain-mediated PTK signaling in mouse zygotic development

Protein phospholipase C Zeta1 expression in patients with failed ICSI but with normal sperm parameters

Activation of bovine somatic cell nuclear transfer embryos by PLCZ cRNA injection

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