Mammalian Fatty Acid Amides of the Brain and CNS

Waluk, Dominik P.; Battistini, Matthew R.; Dempsey, Daniel R.; Farrell, Emma K.; Jeffries, Kristen A.; Mitchell, Perry; Hernandez, Lucas W.; McBride, Joshua C.; Merkler, David J.; Hunt, Mary C.

doi:10.1016/b978-0-12-410527-0.00009-0

Cited by 11 publications

(12 citation statements)

References 190 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Decreased serum palmitamide in AD was reported earlier [38]. In contrast to oleamide, far less is known about the biological function of palmitamide, linoleamide, and stearamide [26]. Alterations in the blood levels of the PFAMs have been reported for other diseases or disease models.…”

Section: Discussionmentioning

confidence: 93%

“…Oleamide was first identified in brains of sleep-deprived cats and mice [24] and is an important regulatory lipid in the brain and central nervous system. Oleamide regulates the sleep-wake cycle, memory, locomotion, pain perception, and is anti-inflammatory, anxiolytic, and neuroprotective [25,26]. Administration of oleamide protects against scopolamine-induced cognitive impairment [27] and depression in a rat chronic stress model [28].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Primary fatty amides in plasma associated with brain amyloid burden, hippocampal volume, and memory in the European Medical Information Framework for Alzheimer's Disease biomarker discovery cohort

Kim

Snowden

Suvitaival

et al. 2019

Alzheimer's & Dementia

Self Cite

View full text Add to dashboard Cite

Introduction: A critical and as-yet unmet need in Alzheimer’s disease (AD) is the discovery of peripheral small molecule biomarkers. Given that brain pathology precedes clinical symptom onset, we set out to test whether metabolites in blood associated with pathology as indexed by cerebrospinal fluid (CSF) AD biomarkers. Methods: This study analyzed 593 plasma samples selected from the European Medical Information Framework for Alzheimer’s Disease Multimodal Biomarker Discovery study, of individuals who were cognitively healthy (n = 242), had mild cognitive impairment (n = 236), or had AD-type dementia (n = 115). Logistic regressions were carried out between plasma metabolites (n = 883) and CSF markers, magnetic resonance imaging, cognition, and clinical diagnosis. Results: Eight metabolites were associated with amyloid b and one with t-tau in CSF, these were primary fatty acid amides (PFAMs), lipokines, and amino acids. From these, PFAMs, glutamate, and aspartate also associated with hippocampal volume and memory. Discussion: PFAMs have been found increased and associated with amyloid b burden in CSF and clinical measures.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Primary fatty amides in plasma associated with brain amyloid burden, hippocampal volume, and memory in the European Medical Information Framework for Alzheimer's Disease biomarker discovery cohort

Kim

Snowden

Suvitaival

et al. 2019

Alzheimer's & Dementia

Self Cite

View full text Add to dashboard Cite

show abstract

“…Much remains unknown about the fatty acid amides: many have no clearly defined physiological function, details regarding their metabolism remain elusive (biosynthesis, degradation, and cellular transport), and the receptor(s) targeted by most are unidentified [1–3,22]. One focus of our research has been on the identification and characterization of enzymes involved in fatty acid amide biosynthesis and melding in vitro substrate specificity data with metabolomic data [23,24].…”

Section: Introductionmentioning

confidence: 99%

Bm -iAANAT and its potential role in fatty acid amide biosynthesis in Bombyx mori

Anderson

Battistini

Wallis

et al. 2018

Prostaglandins, Leukotrienes and Essential Fatty Acids

Self Cite

View full text Add to dashboard Cite

The purpose of this research is to unravel the substrate specificity and kinetic properties of an insect arylalkylamine N-acyltransferase from Bombyx mori (Bm-iAANAT) and to determine if this enzyme will catalyze the formation of long chain N-acylarylalkylamides in vitro. However, the determination of substrates and products for Bm-iAANAT in vitro is no guarantee that these same molecules are substrates and products for the enzyme in the organism. Therefore, RT-PCR was performed to detect the Bm-iAANAT transcripts and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QToF-MS) analysis was performed on purified lipid extracts from B. mori larvae (fourth instar, Bmi4) to determine if long chain fatty acid amides are produced in B. mori. Ultimately, we found that recombinant Bm-iAANAT will utilize long-chain acyl-CoA thioesters as substrates and identified Bm-iAANAT transcripts and long-chain fatty acid amides in Bmi4. Together, these data show Bm-iAANAT will catalyze the formation of long-chain N-acylarylalkylamides in vitro and provide evidence demonstrating that Bm-iAANAT has a role in fatty acid amide biosynthesis in B. mori, as well.

show abstract

“…Hundreds of different fatty acid amides are possible and, to date, approximately 90 different fatty acid amides have been identified from living organisms [ 1 ]. In vivo, accumulating evidence suggests that the fatty acid amides are cell signaling lipids [ 2 , 3 , 4 , 5 , 6 ] and have a technological use as slip additives in plastics [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources

Bhandari

Mitchell

et al. 2021

Molecules

Self Cite

View full text Add to dashboard Cite

Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, the methods for the chemical synthesis and subsequent characterization of specific members of the fatty acid amide family are described. The synthetically prepared fatty acid amides and those obtained commercially are used as standards for the characterization and quantification of the fatty acid amides produced by biological systems, a fatty acid amidome. The fatty acid amidomes from mouse N18TG2 cells, sheep choroid plexus cells, Drosophila melanogaster, Bombyx mori, Apis mellifera, and Tribolium castaneum are presented.

show abstract

Mammalian Fatty Acid Amides of the Brain and CNS

Cited by 11 publications

References 190 publications

Primary fatty amides in plasma associated with brain amyloid burden, hippocampal volume, and memory in the European Medical Information Framework for Alzheimer's Disease biomarker discovery cohort

Primary fatty amides in plasma associated with brain amyloid burden, hippocampal volume, and memory in the European Medical Information Framework for Alzheimer's Disease biomarker discovery cohort

Bm -iAANAT and its potential role in fatty acid amide biosynthesis in Bombyx mori

Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources

Contact Info

Product

Resources

About