Mammalian animal models for dengue virus infection: a recent overview

Kayesh, Mohammad Enamul Hoque; Tsukiyama-Kohara, Kyoko

doi:10.1007/s00705-021-05298-2

Cited by 27 publications

(20 citation statements)

References 141 publications

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“…Preexisting immunities play distinct roles in subsequent viral pathogenesis and even outcome. The studies conducted on different models always draw different conclusions: prior JEV immunity offered cross-protection against DENV in mice [38], while a contrary conclusion was obtained in the population study [50] but, as an acknowledged limitation, there is no ideal mouse model to investigate the ADE [63]. Therefore, no symptom or complete survival in the mouse model does not signify that sequential infections of flaviviruses are dependable for human beings [5].…”

Section: Model Effect Probable Mechanismmentioning

confidence: 99%

Cross-Reactive Immunity among Five Medically Important Mosquito-Borne Flaviviruses Related to Human Diseases

Hou

Chen

Gao

et al. 2022

Viruses

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Flaviviruses cause a spectrum of potentially severe diseases. Most flaviviruses are transmitted by mosquitoes or ticks and are widely distributed all over the world. Among them, several mosquito-borne flaviviruses are co-epidemic, and the similarity of their antigenicity creates abundant cross-reactive immune responses which complicate their prevention and control. At present, only effective vaccines against yellow fever and Japanese encephalitis have been used clinically, while the optimal vaccines against other flavivirus diseases are still under development. The antibody-dependent enhancement generated by cross-reactive immune responses against different serotypes of dengue virus makes the development of the dengue fever vaccine a bottleneck. It has been proposed that the cross-reactive immunity elicited by prior infection of mosquito-borne flavivirus could also affect the outcome of the subsequent infection of heterologous flavivirus. In this review, we focused on five medically important flaviviruses, and rearranged and recapitulated their cross-reactive immunity in detail from the perspectives of serological experiments in vitro, animal experiments in vivo, and human cohort studies. We look forward to providing references and new insights for the research of flavivirus vaccines and specific prevention.

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Section: Model Effect Probable Mechanismmentioning

confidence: 99%

Cross-Reactive Immunity among Five Medically Important Mosquito-Borne Flaviviruses Related to Human Diseases

Hou

Chen

Gao

et al. 2022

Viruses

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“…We verified the role of vimentin in DENV invasion in SV129 suckling mice with and without vimentin knockout. Many models have been used to study DENV, including A/J ( Shresta et al., 2004 ), BALB/c ( Ahmad et al., 2019 ), C57BL/6 ( Byrne et al., 2020 ), and AG129 ( Tan et al., 2010 ) mice for dengue virus tropism and pathogenic research; the DENV-infected non-human primate ( Kayesh and Tsukiyama-Kohara, 2022 ), AG129 mouse continuous infection, cross-infection-established ADE mouse models to study the immune mechanisms of DENV infection ( Blaney et al., 2002 ); and DENV-infected suckling mouse ( Pelliccia et al., 2017 ) and DENV-infected SCID mouse models in vaccine research. In this study, we used suckling mice because adult mice displayed no obvious clinical signs after infection, and DENV-2 was not detected in the serum.…”

Section: Discussionmentioning

confidence: 99%

Vimentin Inhibits Dengue Virus Type 2 Invasion of the Blood-Brain Barrier

Zhou

et al. 2022

Front. Cell. Infect. Microbiol.

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Dengue virus (DENV) causes dengue fever, which is prevalent in the tropical and subtropical regions, and in recent years, has resulted in several major epidemics. Vimentin, a cytoskeletal component involved in DENV infection, is significantly reorganized during infection. However, the mechanism underlying the association between DENV infection and vimentin is still poorly understood. We generated vimentin-knockout (Vim-KO) human brain microvascular endothelial cells (HBMECs) and a Vim-KO SV129 suckling mouse model, combining the dynamic vimentin changes observed in vitro and differences in disease course in vivo, to clarify the role of vimentin in DENV-2 infection. We found that the phosphorylation and solubility of vimentin changed dynamically during DENV-2 infection of HBMECs, suggesting the regulation of vimentin by DENV-2 infection. The similar trends observed in the phosphorylation and solubility of vimentin showed that these characteristics are related. Compared with that in control cells, the DENV-2 viral load was significantly increased in Vim-KO HBMECs, and after DENV-2 infection, Vim-KO SV129 mice displayed more severe disease signs than wild-type SV129 mice, as well as higher viral loads in their serum and brain tissue, demonstrating that vimentin can inhibit DENV-2 infection. Moreover, Vim-KO SV129 mice had more disordered cerebral cortical nerve cells, confirming that Vim-KO mice were more susceptible to DENV-2 infection, which causes severe brain damage. The findings of our study help clarify the mechanism by which vimentin inhibits DENV-2 infection and provides guidance for antiviral treatment strategies for DENV infections.

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“…Papillomavirus infections have also been shown to alter the levels of these miRNAs in cells, having important effects on the p53 signaling pathway (22)(23)(24). Even though studies have shown that DENV is harmful (25,26), the molecular basis of apoptosis is not well understood. miRNA-15 is a lot like miRNA-16, which is also involved in apoptotic pathways and cell growth during oncogenic processes (25,27).…”

Section: Discussionmentioning

confidence: 99%

Experimental Dengue 4 Infection Increases the Production of NS1 Protein and Expression of MicroRNAs-15/16 Levels, Triggering an Apoptosis Caspase Induced Pathway

Casseb¹,

Melo²,

Carvalho³

et al. 2022

Preprint

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The World Health Organization has estimated an annual occurrence of approximately 392 million dengue virus (DENV) infections in more than 100 countries where the virus is endemic, and this represents a serious threat to humanity. DENV is a serologic group with four distinct serotypes (DENV1, DENV2, DENV3, and DENV4) belonging to the genus Flavivirus, family Flaviviridae. Dengue is the most widespread mosquito-borne disease in the world. The ~10.7 kb DENV genome encodes three structural proteins (capsid [C], pre-membrane [prM], and envelope [E]) and seven non-structural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). The NS1 protein is found both as a membrane-associated dimer and as a secreted, lipid-associated hexamer. Dimeric NS1 is found on membranes both in cellular compartments and on the cell surface. Secreted NS1 (sNS1) is often present in patient serum at very high levels, which correlates with severe dengue symptoms. This study was carried out to find out how the NS1 protein, miRNAs 15 and 16, and apoptosis are related to each other during DENV4 infection in human liver cell line culture. The Huh 7.5 and HepG2 strains were infected with DENV4, and after different times of infection, miRNA-15 and miRNA-16, viral load, NS1 protein, and caspases 3 and 7 were quantified. This study demonstrated that miRNAs 15 and 16 are overexpressed during infection of HepG2 and HuH7.5 cells by DENV4 and have a relationship with NS1 protein expression, VDEN4 viral load, and caspase pathways 3 and 7, thus making these miRNAs interesting targets for markers of injuries during VDEN infection in human hepatocyte cells.

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Mammalian animal models for dengue virus infection: a recent overview

Cited by 27 publications

References 141 publications

Cross-Reactive Immunity among Five Medically Important Mosquito-Borne Flaviviruses Related to Human Diseases

Cross-Reactive Immunity among Five Medically Important Mosquito-Borne Flaviviruses Related to Human Diseases

Vimentin Inhibits Dengue Virus Type 2 Invasion of the Blood-Brain Barrier

Experimental Dengue 4 Infection Increases the Production of NS1 Protein and Expression of MicroRNAs-15/16 Levels, Triggering an Apoptosis Caspase Induced Pathway

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