Mammaglobin Expression in Gynecologic Malignancies and Malignant Effusions Detected by Nested Reverse Transcriptase-Polymerase Chain Reaction

Grünewald, Kurt; Haun, Margot; Fiegl, Michael; Urbanek, M; Müller‐Holzner, Elisabeth; Massoner, Anita; Riha, Karin; Propst, A; Marth, Christian; Gastl, Günther

doi:10.1097/01.lab.0000027840.16064.8a

Cited by 39 publications

(33 citation statements)

References 19 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may explain the occurrence of a single false-positive result in a healthy donor sample. In addition, other groups reported the detection of mammaglobin transcript in non-breast tissues, such as ovary, uterus, cervix and sebaceous glands [37,39,40].…”

Section: Discussionmentioning

confidence: 99%

Detection of human mammaglobin mRNA in serial peripheral blood samples from patients with non-metastatic breast cancer is not predictive of disease recurrence

Marques

Teixeira

Diamond

et al. 2008

Breast Cancer Res Treat

View full text Add to dashboard Cite

International audienceHuman mammaglobin (hMAM) mRNA is a sensitive and specific marker of breast cancer cells. We evaluated if hMAM mRNA detection in serial peripheral blood samples from non-metastatic breast cancer patients predicts for disease recurrence. Patients scheduled for adjuvant or neoadjuvant chemotherapy were eligible. Serial blood samples were collected up to 5 years, the first before (neo)adjuvant chemotherapy. hMAM gene expression was analysed by RT-PCR. Specificity was evaluated in blood samples from healthy volunteers. A total of 321 patients were included. The incidence of pre-chemotherapy hMAM-positive samples was similar in patients who latter experienced cancer recurrence (22.4%) and those who remained disease-free (17.9%; = 0.46). Similarly, the mean number of positive follow-up samples was similar in both groups (0.15 ± 0.22 and 0.13 ± 013; = 0.29). Furthermore, there was no difference in disease-free (= 0.63) or overall survival (= 0.57) in patients with and without positive baseline samples or between patients whose follow-up samples were always hMAM negative and those with at least one positive sample. Multivariate survival analysis confirmed that hMAM mRNA detection before or after (neo)adjuvant chemotherapy was not predictive of recurrence. There is no evidence that hMAM mRNA detection at diagnosis or during follow-up predicts for breast cancer recurrence

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of human mammaglobin mRNA in serial peripheral blood samples from patients with non-metastatic breast cancer is not predictive of disease recurrence

Marques

Teixeira

Diamond

et al. 2008

Breast Cancer Res Treat

View full text Add to dashboard Cite

show abstract

“…Gruenewald et al 11 reported that MGB1 is also expressed in normal and cancerous tissues of the ovary, endometrium, and uterine cervix, as well as in normal breast and breast cancer tissues. Indeed, a metastatic endometrial cancer in our series expressed this molecule.…”

Section: Discussionmentioning

confidence: 99%

“…Only a few articles have described these molecules, but they support breast-specific expression. [11][12][13][14][15] However, both SBEM and PDEF could be detected in normal lung. Such a discrepancy might result from our highly sensitive RT-PCR analysis, which we used to obviate the possibility of pseudo-tissue-specific expression due to a low level of expression.…”

Section: Discussionmentioning

confidence: 99%

“…Although only a few articles have described these molecules, they support breastspecific expression. [11][12][13][14][15] Detailed results from the SAGE database and the literature are summarized in Table 1. We next examined whether expression of these molecules could be used as practical distinguishing markers between primary lung cancers and metastatic breast cancers.…”

Section: Searching For Sensitive Markers Of Metastatic Breast Cancermentioning

confidence: 99%

See 1 more Smart Citation

Identification of MGB1 as a Marker in the Differential Diagnosis of Lung Tumors in Patients with a History of Breast Cancer by Analysis of Publicly Available SAGE Data

Koga

Horio

Mitsudomi

et al. 2004

The Journal of Molecular Diagnostics

View full text Add to dashboard Cite

The risk of developing second primary cancers is increased in patients with breast cancer. The lung is one of the major target organs, and therefore a differential diagnosis between primary and metastatic cancers is required for the treatment of lung tumors in patients with a history of breast cancer. However, biopsy specimens frequently result in small, fragmented tissues containing only a few, degenerated cancer cells. We attempted to find a useful marker for differential diagnosis, using the online SAGE database. We selected three molecules, small breast epithelial mucin (SBEM), prostate epithelium-specific Ets transcription factor (PDEF), and mammaglobin (MGB1), as potential markers for breast cancer. SBEM and PDEF proved of no use for practical differential diagnosis because they are expressed in the normal bronchus. In contrast, expression of MGB1 was detected in all 22 primary breast cancers, but not in 22 normal lung tissues. Furthermore, all 12 metastatic breast cancers examined demonstrated positive MGB1 transcripts, whereas one of 48 primary lung adenocarcinomas expressed MGB1. This suggests that MGB1 can serve as a differential molecular marker. In practice, prospective examination, using the nine cases with a history of breast cancer, confirmed the usefulness of MGB1 in differential diagnosis.

show abstract

“…It has a relative breastspecific expression because it is also detectable in ovary, endometrial and eccrine sweat gland tissues (Watson and Fleming, 1996;Grünewald et al, 2002;Sjodin et al, 2003).…”

mentioning

confidence: 99%

Effect of different cytokines on mammaglobin and maspin gene expression in normal leukocytes: possible relevance to the assays for the detection of micrometastatic breast cancer

et al. 2005

View full text Add to dashboard Cite

In cancer patients, the ability to detect disseminated tumour cells in peripheral blood or bone marrow could improve prognosis and consent both early detection of metastatic disease and monitoring of the efficacy of systemic therapy. These objectives remain elusive mainly due to the lack of specific genetic markers for solid tumours. The use of surrogate tissue-specific markers can reduce the specificity of the assays and give rise to a clinically unacceptable false-positive rate. Mammaglobin (MAM) and maspin are two putative breast tissue-specific markers frequently used for detection of occult tumour cells in the peripheral blood, bone marrow and lymph nodes of breast cancer patients. In this study, it was evaluated whether MAM and maspin gene expression may be induced in the normal blood and bone marrow cells exposed to a panel of cytokines, including chemotactic factors (C5a, interleukin (IL)-8), LPS, proinflammatory cytokines (TNF-a, IL-1b) and growth factors (IL-3, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor). The experimental data show that all cytokines included in the panel, except for IL-8, were able to induce maspin expression; on the contrary, MAM gene was never induced. These results suggest that MAM is more specific than maspin and that the possible interference of cytokines should be taken into account in interpreting molecular assays for detection of isolated tumour cells.

show abstract

Mammaglobin Expression in Gynecologic Malignancies and Malignant Effusions Detected by Nested Reverse Transcriptase-Polymerase Chain Reaction

Cited by 39 publications

References 19 publications

Detection of human mammaglobin mRNA in serial peripheral blood samples from patients with non-metastatic breast cancer is not predictive of disease recurrence

Detection of human mammaglobin mRNA in serial peripheral blood samples from patients with non-metastatic breast cancer is not predictive of disease recurrence

Identification of MGB1 as a Marker in the Differential Diagnosis of Lung Tumors in Patients with a History of Breast Cancer by Analysis of Publicly Available SAGE Data

Effect of different cytokines on mammaglobin and maspin gene expression in normal leukocytes: possible relevance to the assays for the detection of micrometastatic breast cancer

Contact Info

Product

Resources

About