Malsoor Virus, a Novel Bat Phlebovirus, Is Closely Related to Severe Fever with Thrombocytopenia Syndrome Virus and Heartland Virus

Mourya, Devendra T.; Yadav, Pragya D; Basu, Atanu; Shete, Anita M.; Patil, Deepak Y.; Zawar, Divya; Majumdar, Triparna; Kokate, Prasad; Sarkale, Prasad; Raut, Chandrashekhar G.; Jadhav, Santosh

doi:10.1128/jvi.02617-13

Cited by 80 publications

(59 citation statements)

References 26 publications

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“…However, it is also possible that at least some of these results are attributable to circulation of a different, unidentified, phlebovirus. Mourya et al [30] reported a novel bat phlebovirus named Malsoor virus, related to severe fever with thrombocytopenia syndrome virus (SFTSV). Malsoor virus was isolated from a related fruit bat ( Rousettus leschenaultia ) in Western India [30].…”

Section: Discussionmentioning

confidence: 99%

“…Mourya et al [30] reported a novel bat phlebovirus named Malsoor virus, related to severe fever with thrombocytopenia syndrome virus (SFTSV). Malsoor virus was isolated from a related fruit bat ( Rousettus leschenaultia ) in Western India [30]. Mossel et al [21] reported two isolations of Arumowot virus ( Phenuiviridae, Phlebovirus ) from Culex mosquitoes collected in Jinja, Uganda, in 2012.…”

Section: Discussionmentioning

confidence: 99%

“…Mossel et al [21] reported two isolations of Arumowot virus ( Phenuiviridae, Phlebovirus ) from Culex mosquitoes collected in Jinja, Uganda, in 2012. While Malsoor and Arumowot viruses are both distantly related to RVFV [30], we did not evaluate the potential for cross-reactivity of these samples to other phleboviruses. The ability of Egyptian rousette bats and little epauletted fruit bats to become infected with RVFV and develop viremias high enough to infect feeding mosquitoes is unknown.…”

Section: Discussionmentioning

confidence: 99%

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Neutralizing antibodies against flaviviruses, Babanki virus, and Rift Valley fever virus in Ugandan bats

Kading

Kityo

Mossel

et al. 2018

Infection Ecology & Epidemiology

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Introduction: A number of arboviruses have previously been isolated from naturally-infected East African bats, however the role of bats in arbovirus maintenance is poorly understood. The aim of this study was to investigate the exposure history of Ugandan bats to a panel of arboviruses. Materials and methods: Insectivorous and fruit bats were captured from multiple locations throughout Uganda during 2009 and 2011–2013. All serum samples were tested for neutralizing antibodies against West Nile virus (WNV), yellow fever virus (YFV), dengue 2 virus (DENV-2), Zika virus (ZIKV), Babanki virus (BBKV), and Rift Valley fever virus (RVFV) by plaque reduction neutralization test (PRNT). Sera from up to 626 bats were screened for antibodies against each virus. Results and Discussion: Key findings include the presence of neutralizing antibodies against RVFV in 5/52 (9.6%) of little epauletted fruit bats (Epomophorus labiatus) captured from Kawuku and 3/54 (5.6%) Egyptian rousette bats from Kasokero cave. Antibodies reactive to flaviviruses were widespread across bat taxa and sampling locations. Conclusion: The data presented demonstrate the widespread exposure of bats in Uganda to arboviruses, and highlight particular virus-bat associations that warrant further investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Neutralizing antibodies against flaviviruses, Babanki virus, and Rift Valley fever virus in Ugandan bats

Kading

Kityo

Mossel

et al. 2018

Infection Ecology & Epidemiology

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“…Sequence analysis showed highest homology with known sequences of Phleboviruses and was found to be distinctly close to Heartland and severe fever with thromocytopenia syndrome (SFTS) viruses12.…”

Section: Resultsmentioning

confidence: 84%

Development of polymerase chain reaction-based diagnostic tests for detection of Malsoor virus & adenovirus isolated from Rousettus species of bats in Maharashtra, India

et al. 2017

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Background & objectives:Bats are recognized as important reservoirs for emerging infectious disease and some unknown viral diseases. Two novel viruses, Malsoor virus (family Bunyaviridae, genus, Phlebovirus) and a novel adenovirus (AdV) (family, Adenoviridae genus, Mastadenovirus), were identified from Rousettus bats in the Maharashtra State of India. This study was done to develop and optimize real time reverse transcription - polymerase chain reaction (RT-PCR) assays for Malsoor virus and real time and nested PCR for adenovirus from Rousettus bats.Methods:For rapid and accurate screening of Malsoor virus and adenovirus a nested polymerase chain reaction and TaqMan-based real-time PCR were developed. Highly conserved region of nucleoprotein gene of phleboviruses and polymerase gene sequence from the Indian bat AdV isolate polyprotein gene were selected respectively for diagnostic assay development of Malsoor virus and AdV. Sensitivity and specificity of assays were calculated and optimized assays were used to screen bat samples.Results:Molecular diagnostic assays were developed for screening of Malsoor virus and AdV and those were found to be specific. Based on the experiments performed with different parameters, nested PCR was found to be more sensitive than real-time PCR; however, for rapid screening, real-time PCR can be used and further nested PCR can be used for final confirmation or in those laboratories where real-time facility/expertise is not existing.Interpretation & conclusions:This study reports the development and optimization of nested RT-PCR and a TaqMan-based real-time PCR for Malsoor virus and AdV. The diagnostic assays can be used for rapid detection of these novel viruses to understand their prevalence among bat population.

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“…In the latter case, the virus-termed SFTSV strain YG1-was isolated from a patient who died from a febrile illness of unknown etiology, although since the patient had never travelled abroad, she is thought to have been infected in Japan (24). Notably, two cases of a similar febrile illness with thrombocytopenia have been reported in Missouri, USA, and the causative agent, named Heartland virus, was determined to be a novel tick-borne phlebovirus closely related to SFTSV (18,19). SFTSV belongs to the genus Phlebovirus of the family Bunyaviridae (27,29).…”

mentioning

confidence: 99%

<b>The amino acid at position 624 in the glycoprotein of SFTSV (severe fever with thrombocytopenia virus) plays a critical role in low-pH-dependent cell fusion </b><b>activity </b>

et al. 2017

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Severe fever with thrombocytopenia syndrome virus (SFTSV) is a novel phlebovirus responsible for causing an emerging zoonotic disease. We previously established subclones from SFTSV strain YG1 based on differences in low-pH-dependent cell fusion activities and found two amino acid substitutions, Y328H and R624W, in the envelope glycoprotein (GP) of high fusion subclones. In this study, we show that transiently expressed GP with the R624W mutation, but not the Y328H mutation, induced cell fusion under acidic conditions. GP possessing either tryptophan, serine, glycine or aspartic acid at position 624 induced cell fusion, whereas GP possessing basic amino acids such as arginine or lysine did not induce cell fusion. These results indicated that the amino acid at position 624 has an important role for inducing low-pH-dependent cell fusion.

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Malsoor Virus, a Novel Bat Phlebovirus, Is Closely Related to Severe Fever with Thrombocytopenia Syndrome Virus and Heartland Virus

Cited by 80 publications

References 26 publications

Neutralizing antibodies against flaviviruses, Babanki virus, and Rift Valley fever virus in Ugandan bats

Neutralizing antibodies against flaviviruses, Babanki virus, and Rift Valley fever virus in Ugandan bats

Development of polymerase chain reaction-based diagnostic tests for detection of Malsoor virus & adenovirus isolated from Rousettus species of bats in Maharashtra, India

<b>The amino acid at position 624 in the glycoprotein of SFTSV (severe fever with thrombocytopenia virus) plays a critical role in low-pH-dependent cell fusion </b><b>activity </b>

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