Malonylome analysis of rhizobacterium Bacillus amyloliquefaciens FZB42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions

Fan, Bin; Li, Yulong; Li, Lei; Peng, Xiaojun; Bu, Chen; Wu, Xiao‐Qin; Borriss, Rainer

doi:10.1016/j.jprot.2016.11.022

Cited by 38 publications

(40 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The polyketide synthases, BmyA, BaeM, BaeN, and BaeR contain more than 10 malonylation sites. BaeR is the most highly malonylated protein carrying 17 malonylation sites ( Fan et al, 2017b , c ).…”

Section: Protein Modificationmentioning

confidence: 99%

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

et al. 2018

Self Cite

View full text Add to dashboard Cite

Bacillus velezensis FZB42, the model strain for Gram-positive plant-growth-promoting and biocontrol rhizobacteria, has been isolated in 1998 and sequenced in 2007. In order to celebrate these anniversaries, we summarize here the recent knowledge about FZB42. In last 20 years, more than 140 articles devoted to FZB42 have been published. At first, research was mainly focused on antimicrobial compounds, apparently responsible for biocontrol effects against plant pathogens, recent research is increasingly directed to expression of genes involved in bacteria–plant interaction, regulatory small RNAs (sRNAs), and on modification of enzymes involved in synthesis of antimicrobial compounds by processes such as acetylation and malonylation. Till now, 13 gene clusters involved in non-ribosomal and ribosomal synthesis of secondary metabolites with putative antimicrobial action have been identified within the genome of FZB42. These gene clusters cover around 10% of the whole genome. Antimicrobial compounds suppress not only growth of plant pathogenic bacteria and fungi, but could also stimulate induced systemic resistance (ISR) in plants. It has been found that besides secondary metabolites also volatile organic compounds are involved in the biocontrol effect exerted by FZB42 under biotic (plant pathogens) and abiotic stress conditions. In order to facilitate easy access to the genomic data, we have established an integrating data bank ‘AmyloWiki’ containing accumulated information about the genes present in FZB42, available mutant strains, and other aspects of FZB42 research, which is structured similar as the famous SubtiWiki data bank.

show abstract

Section: Protein Modificationmentioning

confidence: 99%

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Post-translational modifications (PTMs) are dynamic and reversible modifications of proteins and have wide effects in broadening the range of functionality of these proteins [ 1 – 3 ]. Many functional groups, such as phospho, ubiquityl, acetyl, methyl, and malonyl groups, are able to be introduced by PTMs [ 4 ]. Among PTMs, protein malonylation is a newly discovered modification, playing an important role in modulating various cellular processes [ 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Many functional groups, such as phospho, ubiquityl, acetyl, methyl, and malonyl groups, are able to be introduced by PTMs [ 4 ]. Among PTMs, protein malonylation is a newly discovered modification, playing an important role in modulating various cellular processes [ 4 – 6 ]. Through reversible addition of a malonyl group to lysine residues, protein malonylation regulates protein localization, enzymatic activity, protein stability and many other biochemical processes.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, as a result of advancements in liquid chromatography-tandem mass spectrometry (LC-MS/MS), a large number of lysine-acetylated proteins have been identified [ 2 ]. However, compared to these acetylation profiles, only a few organisms have been studied with respect to lysine malonylation, including Bacillus amyloliquefaciens [ 4 ], Escherichia coli [ 5 ], Saccharopolyspora erythraea [ 10 ], cyanobacteria ( Synechocystis ) [ 11 ], rice [ 12 ], HeLa cells [ 9 ], and mice [ 7 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Systematic analysis of the lysine malonylome in common wheat

et al. 2018

View full text Add to dashboard Cite

BackgroundProtein lysine malonylation, a newly discovered post-translational modification (PTM), plays an important role in diverse metabolic processes in both eukaryotes and prokaryotes. Common wheat is a major global cereal crop. However, the functions of lysine malonylation are relatively unknown in this crop. Here, a global analysis of lysine malonylation was performed in wheat.ResultsIn total, 342 lysine malonylated sites were identified in 233 proteins. Bioinformatics analysis showed that the frequency of arginine (R) in position + 1 was highest, and a modification motif, KmaR, was identified. The malonylated proteins were located in multiple subcellular compartments, especially in the cytosol (45%) and chloroplast (30%). The identified proteins were found to be involved in diverse pathways, such as carbon metabolism, the Calvin cycle, and the biosynthesis of amino acids, suggesting an important role for lysine malonylation in these processes. Protein interaction network analysis revealed eight highly interconnected clusters of malonylated proteins, and 137 malonylated proteins were mapped to the protein network database. Moreover, five proteins were simultaneously modified by lysine malonylation, acetylation and succinylation, suggesting that these three PTMs may coordinately regulate the function of many proteins in common wheat.ConclusionsOur results suggest that lysine malonylation is involved in a variety of biological processes, especially carbon fixation in photosynthetic organisms. These data represent the first report of the lysine malonylome in common wheat and provide an important dataset for further exploring the physiological role of lysine malonylation in wheat and likely all plants.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4535-y) contains supplementary material, which is available to authorized users.

show abstract

“…The developmental stages of plant pathogens have been shown to characteristically differ at the transcriptional, post-transcriptional, translational, and post-translational levels (31,(73)(74)(75). In particular, N-glycosylation is a post-translational modification critical for fundamental processes underlying growth and reproduction (28)(29)(30).…”

Section: Most Of the 496 N-glycosylation Sites Matched With The Consementioning

confidence: 99%

The consensus N_glyco-X-S/T motif and a previously unknown N_glyco-N -linked glycosylation are necessary for growth and pathogenicity ofPhytophthora

Zhang

Chen

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Asparagine (Asn, N) -linked glycosylation within the glycosylation motif (Nglyco-X-S/T; X P) is a ubiquitously distributed post-translational modification that participates in diverse eukaryotic cellular processes. However, little is known about the characteristic features and roles of N-glycosylation in oomycetes. In this work, it found that 2.5 μg/ml tunicamycin (N-glycosylation inhibitor) completely inhibited Phytophthora sojae growth, suggesting that N-glycosylation is necessary for oomycete development. We conducted a glycoproteomic analysis of P. sojae to identify and map all N-glycosylated proteins and to quantify differentially expressed glycoproteins associated with mycelia, asexual cysts, and sexual oospores. A total of 355 N-glycosylated proteins were found, containing 496 glycosites that likely participate in glycan degradation, carbon metabolism, glycolysis, or other central metabolic pathways. To verify the glycoproteomic results and further examine the function of N-glycosylation in P. sojae, two proteins were selected for PNGase F deglycosylation assays and CRISPR/Cas9-mediated site-directed mutagenesis, including a GPI transamidase protein (GPI16) up-regulated in cysts, with the consensus Nglyco-X-S/T motif at Asn 94, and a heat shock protein 70 (HSP70) up-regulated in cysts and oospores with a previously unknown Nglyco-N motif at Asn 270. We demonstrated that the GPI16 and HSP70 are both N-glycosylated proteins, confirming that the Nglyco-N motif is a target site for asparagine -oligosaccharide N-glycosidic linkage. Glycosite mutations of Asn 94 in the GPI16 led to impaired cyst germination and pathogenicity, while HSP70 mutants exhibited decreased cyst germination and oospore production. This work describes an integrated map of oomycete N-glycoproteomes and advances our understanding of N-glycosylation in oomycetes. Moreover, we confirm that the consensus Nglyco-X-S/T and the Nglyco-N -linked glycosites are both essential for the growth of Phytophthora sojae, indicating that there are multiple N-glycosylation motifs in oomycetes.

show abstract

Malonylome analysis of rhizobacterium Bacillus amyloliquefaciens FZB42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions

Cited by 38 publications

References 84 publications

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

Systematic analysis of the lysine malonylome in common wheat

The consensus N_glyco-X-S/T motif and a previously unknown N_glyco-N -linked glycosylation are necessary for growth and pathogenicity ofPhytophthora

Contact Info

Product

Resources

About

Malonylome analysis of rhizobacterium Bacillus amyloliquefaciens FZB42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions

Cited by 38 publications

References 84 publications

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

Bacillus velezensis FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol

Systematic analysis of the lysine malonylome in common wheat

The consensus Nglyco-X-S/T motif and a previously unknown Nglyco-N -linked glycosylation are necessary for growth and pathogenicity ofPhytophthora

Contact Info

Product

Resources

About

The consensus N_glyco-X-S/T motif and a previously unknown N_glyco-N -linked glycosylation are necessary for growth and pathogenicity ofPhytophthora