Malonyl-ginsenoside content of a cell-suspension culture of Panax japonicus var. repens

Кочкин, Д. В.; Качала, В. В.; Shashkov, Alexander S.; Chizhov, Alexander O.; Chirva, V. Ya.; Носов, А. М.

doi:10.1016/j.phytochem.2013.03.021

Cited by 34 publications

(34 citation statements)

References 43 publications

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“…repens in fl asks was studied by RP-HPLC-UV analysis [ 166 ]. Seven ginsenosides were identifi ed: Rg 1 , R 0 , malonyl-Rb 1 , Rb 1 , Rc, Rb 2 and Rd.…”

Section: Malonyl-ginsenosides In Ginseng Cell Culturesmentioning

confidence: 99%

“…While the total amount of the ginsenosides varied over a wide range (5.0-49.4 mg g −1 dry weight), the ratios of the major groups remained nearly constant: Rg 1 , R 0 , and malonyl-Rb 1 accounted for 75-93 % of the total ginsenoside content [ 166 ]. These fi ndings suggested that both neutral and malonylated ginsenosides should be analyzed to determine actual ginsenoside content in cell-suspension culture.…”

Section: Malonyl-ginsenosides In Ginseng Cell Culturesmentioning

confidence: 99%

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Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compartmentalized within a single heterotrophic cell with sparse or underdeveloped vacuoles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization.Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.

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“…repens in fl asks was studied by RP-HPLC-UV analysis [ 166 ]. Seven ginsenosides were identifi ed: Rg 1 , R 0 , malonyl-Rb 1 , Rb 1 , Rc, Rb 2 and Rd.…”

Section: Malonyl-ginsenosides In Ginseng Cell Culturesmentioning

confidence: 99%

Section: Malonyl-ginsenosides In Ginseng Cell Culturesmentioning

confidence: 99%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

Self Cite

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“…Ginsenosides accumulate in the root of the plant, but the agricultural production of roots is expensive. Therefore, the production of ginsenosides, by means of different biotechnological alternatives, has been extensively studied by a number of researchers, using callus tissues (Mallol et al 2001 ), cell suspensions (Mathur et al 1994 ;Kochkin et al 2013 ), and root cultures (Yoshikawa and Furuya 1987 ); nevertheless, the productivity obtained so far has been low because of the low growth rates of cultures. The induction and establishment of hairy roots after the infection of Panax ginseng rhizomes with Agrobacterium rhizogenes has been successfully performed (Washida et al 1998 ).…”

Section: Ginsenoside Productionmentioning

confidence: 99%

“…Several TIAs' biosynthesis genes have also been overexpressed in C. roseus hairy root cultures (Zhao et al 2012a, b ). Kochkin et al ( 2013 ) demonstrated for the fi rst time the presence of large amounts of ginsenosides malonyl-Rb1, malonyl-Rc, malonylRb2, and malonyl-Rd in a suspension culture of Panax japonicus var. repens cell.…”

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confidence: 99%

Improving Secondary Metabolite Production in Tissue Cultures

Kumar

2015

Plant Biology and Biotechnology

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Plant cell and tissue culture has been suggested as an alternative means for year-round production of secondary metabolites with an added potential of increasing yields by culture selection and manipulation, genetic transformation, hairy root cultures, and use of bioreactors for mass production. Secondary metabolite pathways and genes involved in those pathways have been identifi ed, and regulation of transcription and transcription factors has been determined by studying functional genomics in conjunction with data-mining tools of bioinformatics. Besides this, advances in metabolic engineering enable researchers to confer new secondary metabolic pathways to crops by transferring three to fi ve, or more, heterologous genes taken from various other species. As an alternative, the metabolic pathways of useful secondary metabolites have been modifi ed to improve their productivity via genetic transformation. However, there is a need to understand metabolic pathways of secondary metabolism at the molecular level. Plant hairy roots offer a novel and sustainable tissue-based system that preserves multiple specialized cell types believed to be important in maintaining a better consistency in synthesis of bioactive secondary molecules. This paper will review stateof-the-art reports on improving production of secondary metabolites in tissue cultures in various plant species.

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“…The formation of ginsenosides in in vitro cells of Panax japonicus var. repens is proposed to be closely related to compartmentation since the accumulation of the PPD-type ginsenosides is strongly dependent on malonylation that targets them to the vacuoles (Kochkin et al 2013 ).…”

Section: Differentiation and Compartmentationmentioning

confidence: 99%

In Vitro Plant Cultures as Biofactories

Álvarez

2014

Plant Biotechnology for Health

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In vitro plant cell cultures are defi ned as a culture in aseptic conditions of any part of the plant body in a nutritive media. Cells, differentiated and undifferentiated (calli and suspended cells) tissues, and organs can be maintained in vitro .Some of the advantages of in vitro cultures are their development and maintenance in defi ned conditions (nutrients, light, humidity, temperature), independent from environmental infl uences (pests, diseases, extreme weather, geographical location, etc.), and manipulated by experienced operators in confi ned facilities.Since its development, in vitro plant cell cultures have been an essential tool for studying plant metabolism, and to micropropagate plant species, maintaining their genetic background. Also, they were helpful to analyze and optimize the production of plant secondary metabolites.Secondary metabolites productive processes are conducted, with a different degree of success, in undifferentiated cultures and in cultures of transformed organs. Several strategies were displayed in order to increase yields as manipulation of the plant growth regulator balance, addition of precursors, biotransformation, and immobilization.Also, as plant cells have a different behavior in liquid culture compared with microorganisms and mammal cells, bioreactor design was adapted to develop plant cell cultures.

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Malonyl-ginsenoside content of a cell-suspension culture of Panax japonicus var. repens

Cited by 34 publications

References 43 publications

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Improving Secondary Metabolite Production in Tissue Cultures

In Vitro Plant Cultures as Biofactories

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