Måling av kalprotektin i feces

Jahnsen, Jørgen; Røseth, Arne; Aadland, E

doi:10.4045/tidsskr.08.0010

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…There is a consensus that patients with organic intestinal diseases can present fCal values above the cut-off point of 50 µg/g, with the value of 100 µg/g or higher being regarded as more specific [ 4 , 22 , 24 ]. Seven patients belonging to this group routinely used aspirin and/or NSAIDs, as well as proton pump inhibitors (such as omeprazole), which can cause side effects in the form of diarrhea, constipation and nausea.…”

Section: Discussionmentioning

confidence: 99%

“…Calprotectin, also known as L1-protein or S100A8/A9, is a small (36 kDa) calcium- and zinc-binding protein mainly found within the cytoplasm of the polymorphonuclear leukocytes, representing 60% of the global cytosolic proteins [ 4 ]. By way of comparison, there is almost as much calprotectin in one granulocyte as there is hemoglobin in a red blood cell.…”

Section: Introductionmentioning

confidence: 99%

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Fecal Calprotectin: A Comparison of Two Commercial Enzymoimmunoassays and Study of Fecal Extract Stability at Room Temperature

et al. 2018

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BackgroundThe aims of the study were: 1) to compare the fecal calprotectin (fCal) assay results with Calprolab™ ELISA (HRP) (Calpro AS) versus our routine method, Elia™ fluoroenzymoimmunoassay (Thermo Fisher), and 2) to determine whether the fCal assay results do not vary following storage of the extract at room temperature for 4 days with the Calpro AS buffer, this being the estimated shipment time from the home of the patient, and an aspect little studied to date.MethodsThe fCal was determined in 198 patients divided into three groups: inflammatory bowel disease (IBD), organic intestinal disease, and functional intestinal disorders. Fecal extraction was carried out using the Roche Diagnostics kit with the corresponding specific buffers.ResultsThe fCal assay with the Thermo Fisher method was found to be more sensitive but less specific than with the Calpro AS technique. The positive predictive value was low (just over 50%), though the negative predictive value was high (over 90%) with both methods. The likelihood ratios revealed small but occasionally important pre- versus post-test differences. When we compared the two methods, the Spearman correlation coefficient (ρ) was 0.819 (95% CI: 0.768 - 0.860) (P < 0.0001), reflecting a positive correlation. Similarly, when stratifying the fCal results into < 50 µg/g, 50 - 100 µg/g and > 100 µg/g, the resulting Cohen’s kappa coefficient was 0.7766 (95% CI: 0.7025 - 0.8507), reflecting a substantial agreement between both methods. The stability of fCal was high in fecal extracts with the Calpro AS extraction buffer at room temperature for 4 days, which yielded a Spearman correlation coefficient of 0.951 (95% CI: 0.933 - 0.965), when the results were compared to those of the recent extracts (P < 0.0001).ConclusionsA positive correlation was observed between the two methods. In view of the high negative predictive value obtained with fCal, the presence of organic disease is highly unlikely in the presence of a normal concentration of this marker. We also confirmed the excellent stability of fCal in fecal extracts with the Calpro AS extraction buffer stored at room temperature. Thus, and for the sake of convenience and hygiene, it would be ideal for the patient to perform the extraction at home.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fecal Calprotectin: A Comparison of Two Commercial Enzymoimmunoassays and Study of Fecal Extract Stability at Room Temperature

et al. 2018

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“…This makes calprotectin an ideal marker in the management of gastrointestinal pathologies. In recent years, faecal calprotectin has received a lot of attention and in the last year alone, more than 50 papers have been published on calprotectin measurements in faeces or other body fluids and tissues [2,5]. In Cameroon, to our knowledge, few studies have been carried out on the measurement of faecal calprotectin in children.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of Faecal Calprotectin in Children with Chronic Gastrointestinal Disorders at Yaounde General Hospital

Isabelle,

Audrey,

Pascal

et al. 2024

AJP

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Introduction: Chronic gastrointestinal disorders are common in children. Numerous faecal biomarkers, such as faecal calprotectin, are used in the aetiological diagnosis of these digestive disorders. The study aimed to investigate the diagnostic value of faecal calprotectin in paediatric chronic gastrointestinal disease compared with that obtained in healthy children. Methodology: This was a comparative, analytical cross-sectional study from October 2022 through June 2023 at Yaoundé General Hospital. Participants were children aged between four and eighteen with chronic digestive disorders. Using a pre-established questionnaire, we collected the socio-demographic and clinical characteristics of each participant. The participants' faecal calprotectin was tested at the laboratory of the Yaoundé University Hospital Center by Enzyme-Linked Immunosorbent Assay (ELISA). Associations between variables were investigated by linear regression and calculation of the odds ratio (OR). The significance threshold was 5%. Results: Sixty stool samples were analysed for faecal calprotectin from 30 patients and 30 healthy participants. The mean age of the population was 9.47 (± 3.35) years for patients and 10.67 (± 3.70) years for healthy participants, with a sex ratio of 1.14 for patients and 0.87 for healthy participants. The threshold value for faecal calprotectin was 2.75 µg/g, with a sensitivity of 60%, a specificity of 63%, a positive predictive value of 61.20% and a negative predictive value of 62.06%. There were no significant differences in faecal calprotectin concentrations between children with chronic gastrointestinal disorders (peptic ulcer disease: p=0.10; functional gastrointestinal disorder associated with peptic ulcer disease: p= 0.710; functional gastrointestinal disorder: p= 0.143) and healthy children. Conclusion: The diagnostic value of faecal calprotectin as a biomarker in the diagnosis of chronic gastrointestinal disease was not observed in this study. However, the biological parameters assessed were measured only once, and given that their concentrations may vary over time, we recommend a subsequent longitudinal study.

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“…Dois valores entre 50 e 200 ug/g também foram considerados indicativos de investigação adicional. 43,44 Níveis de CF ≥ 200 µg/g possuem maior especificidade para doença inflamatória intestinal, mas outras patologias do trato gastrointestinal também podem alterar a calprotectina. 18 Entre elas estão as infecções intestinais agudas, neoplasias, diverticulite, colite microscópica, doença celíaca, pólipos, síndrome do intestino irritável.…”

Section: Calprotectinaunclassified

Prevalência de sintomas gastrointestinais em pacientes com psoríase e a utilização da calprotectina fecal como método de triagem para diagnóstico de doenças inflamatórias intestinais

Helena Kalies Oliveira

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como parte dos requisitos exigidos para a obtenção do título de Mestra em Ciências na área de Clínica Médica. PÁGINA DE AGRADECIMENTOS "O presente trabalho foi realizado com auxílio do Fundo de apoio à dermatologia do estado de São Paulo (FUNADERSP), pertencente à Sociedade Brasileira de Dermatologia -regional de São Paulo." Agradeço à Deus pela conquista. Agradeço ao FUNADERSP pelo apoio e auxílio. Agradeço à Profa. Dra. Raquel Franco Leal e toda sua equipe do laboratório de Doenças Inflamatórias Intestinais no Gastrocentro pelo apoio e auxílio. Agradeço ao Dr. Ciro Garcia Montes e ao Dr. Rafael Dias juntamente com toda a equipe do Centro de Diagnóstico de Doenças do Aparelho Digestivo (Gastrocentro) pelo apoio e auxílio. Agradeço aos médicos residentes e assistentes do ambulatório de Dermatologia da Unicamp pelo apoio e auxílio. Agradeço à Profa. Dra. Renata Ferreira Magalhães pela oportunidade, disponibilidade e orientação.Agradeço à minha família pelo amor, apoio e paciência.

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Måling av kalprotektin i feces

Cited by 10 publications

References 32 publications

Fecal Calprotectin: A Comparison of Two Commercial Enzymoimmunoassays and Study of Fecal Extract Stability at Room Temperature

Fecal Calprotectin: A Comparison of Two Commercial Enzymoimmunoassays and Study of Fecal Extract Stability at Room Temperature

Diagnostic Value of Faecal Calprotectin in Children with Chronic Gastrointestinal Disorders at Yaounde General Hospital

Prevalência de sintomas gastrointestinais em pacientes com psoríase e a utilização da calprotectina fecal como método de triagem para diagnóstico de doenças inflamatórias intestinais

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