Objective:To evaluate the effectiveness of an educational intervention on malignant hyperthermia with operating room nurses. Method: A quasi-experimental study, aimed at an educational intervention of short duration with the nursing staff in the operating room of the institution hosting the research in the city of São Paulo, with the participation of 96 professionals. Pre-intervention tests and post-intervention tests were applied, which consisted of a lecture followed by simulation. Results: Considering the overall results of the intervention, there was a statistically significant difference (p<0.00). After the educational intervention, there was an increase of the minimum and maximum scores, and average growth of 2.64 points in the knowledge of professionals when compared to the previous step. Conclusion: The educational intervention strategy favors the concept of the content developed by everyone involved and qualifies professionals to work safely.