Malignant ascites: pathophysiology and treatment

Cavazzoni, Emanuel; Bugiantella, Walter; Graziosi, Luigina; Franceschini, Maria Silvia; Donini, Annibale

doi:10.1007/s10147-012-0396-6

Cited by 133 publications

(115 citation statements)

References 60 publications

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“…Other cancers, including colon, pancreatic, gastrointestinal tract, lung, and breast, feature tumor cells in ascites and pleural effusion. 17,56 Tumor cells within the ascites are often found at late stages of cancer progression. Previous studies have investigated the impact of shear stress stimulus on a variety of cancer types due to ascitic shear stresses, heightened interstitial fluid flow, and high shear conditions experienced by circulating tumor cells.…”

Section: Alternative Shear Bioreactors For Examining Ovarian Cancementioning

confidence: 99%

Review: Mechanotransduction in ovarian cancer: Shearing into the unknown

2018

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Perspective: The role of mechanobiology in the etiology of brain metastasis APL Bioengineering 2, 031801 (2018) Ovarian cancer remains a deadly diagnosis with an 85% recurrence rate and a 5-year survival rate of only 46%. The poor outlook of this disease has improved little over the past 50 years owing to the lack of early detection, chemoresistance and the complex tumor microenvironment. Within the peritoneal cavity, the presence of ascites stimulates ovarian tumors with shear stresses. The stiff environment found within the tumor extracellular matrix and the peritoneal membrane are also implicated in the metastatic potential and epithelial to mesenchymal transition (EMT) of ovarian cancer. Though these mechanical cues remain highly relevant to the understanding and treatment of ovarian cancers, our current knowledge of their biological processes and their clinical relevance is deeply lacking. Seminal studies on ovarian cancer mechanotransduction have demonstrated close ties between mechanotransduction and ovarian cancer chemoresistance, EMT, enhanced cancer stem cell populations, and metastasis. This review summarizes our current understanding of ovarian cancer mechanotransduction and the gaps in knowledge that exist. Future investigations on ovarian cancer mechanotransduction will greatly improve clinical outcomes via systematic studies that determine shear stress magnitude and its influence on ovarian cancer progression, metastasis, and treatment.

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Section: Alternative Shear Bioreactors For Examining Ovarian Cancementioning

confidence: 99%

Review: Mechanotransduction in ovarian cancer: Shearing into the unknown

2018

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“…For simple QD-based IHC staining, after being blocked with 2% BSA for 30 minutes at 37°C, the slides were incubated with the primary antibodies overnight at 4°C. The slides were then incubated for 2 hours with the corresponding QD probes that were secondary antibodies conjugated with QD on the F(ab') 2 fragments: 1) QD-525 goat F(ab') 2 anti-mouse IgG conjugate (QD-525), which emits green light and whose dilution was 1:200 (Thermo Fisher Scientific, Waltham, MA, USA); 2) QD-585 goat F(ab') 2 antirabbit IgG conjugate (QD-585), which emits yellow light and whose dilution was 1:200 (Thermo Fisher Scientific); and 3) QD-655 rabbit F(ab') 2 anti-goat IgG conjugate (QD-655), which emits red light and whose dilution was 1:500 (Thermo Fisher Scientific).…”

Section: Patients For Gc Tissue Specimens and For Ma Cell Preparationsmentioning

confidence: 99%

“…These are palliative procedures mainly to relieve symptoms, and they have limited efficacy and some degree of risk. 1,2 MA is distinctive exudate containing cancer cells, macrophages, mesothelium cells, lymphocytes, and other cell components. Macrophages and lymphocytes are immunocytes; both have been confirmed to play important roles in cancer progression.…”

Section: Introductionmentioning

confidence: 99%

Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification

Zeng

Peng

Yuan

et al. 2015

IJN

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Purpose The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification. Methods A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features. Results The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-reactive type, cancer cell-dominant type, and cell deletion type; the four categories were statistically different in terms of the ratio of cancer cells to immunocytes ( P <0.001). Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer. Conclusion The newly developed QD-based multiplexed imaging technique was able to better reveal the interactions between cancer cells and immunocytes. This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

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“…Donde intervienen tanto la neovascularización con un aumento de la permeabilidad capilar, obstrucción en el drenaje y producción forzada de líquido peritoneal (12) . Sin olvidar el estímulo de factores como las glicoproteínas, el Factor de Crecimiento Vascular Endotelial (VEGF) Muchos pacientes conascitis malignatieneun alto contenido deproteínas, esto va aalterar la permeabilidad vascular, estohasido implicado tambiénen la patogénesisde la ascitis (5) .…”

Section: Fisiopatología De La Ascitis Malignaunclassified

“…Se considera efectiva en el 40% pacientes con ascitis maligna (6) .Se logra reabsorber con los diuréti-cos un máximo de 800mL por día con una pérdi-da de 1kg/día. Su eficacia es mayor cuando la ascitis es 2º a metástasis hepáticas con hipertensión portal e hipoalbuminemia (12).…”

Section: Abordaje Terapéuticounclassified

CASO 22-2015:Masculino 54 años con ascitis maligna refractaria: uso del catéter venoso central

Méndez¹

2015

RC_UCR-HSJD

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Malignant ascites: pathophysiology and treatment

Cited by 133 publications

References 60 publications

Review: Mechanotransduction in ovarian cancer: Shearing into the unknown

Review: Mechanotransduction in ovarian cancer: Shearing into the unknown

Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification

CASO 22-2015:Masculino 54 años con ascitis maligna refractaria: uso del catéter venoso central

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Malignant ascites: pathophysiology and treatment

Cited by 133 publications

References 60 publications

Review: Mechanotransduction in ovarian cancer: Shearing into the unknown

Review: Mechanotransduction in ovarian cancer: Shearing into the unknown

Quantum dot-based multiplexed imaging in&nbsp;malignant ascites: a new model for malignant ascites classification

CASO 22-2015:Masculino 54 años con ascitis maligna refractaria: uso del catéter venoso central

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Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification