Malignant and transforming activity of rous sarcoma virus. II. The study of variants of rous sarcoma virus isolated from mouse tumours

Obukh, I. B.; Kryukova, I. N.

doi:10.1002/ijc.2910040609

Cited by 5 publications

(2 citation statements)

References 3 publications

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“…Furthermore, the duck-adapted virus had altered surface antigenic properties. A similar phenomenon has also been noted with RSV that has been rescued from transformed rat cells (5)(6)(7)(8)(9)(10)(11)(12). These findings led Altaner and Temin (6) to suggest that the alteration of physical and biological properties in the virus may reflect recombination between viral and host genetic material.…”

supporting

confidence: 71%

Host induced alteration of avian sarcoma virus B-77 genome.

Shoyab¹,

Markham²,

Baluda³

1975

Proc. Natl. Acad. Sci. U.S.A.

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The genome of an avian oncornavirus was altered after infection of a heterologous host. This was studied with avian sarcoma virus B-77 in duck embryonic fibroblasts (DEF) and chicken embryonic fibroblasts (CEF). To detect alteration of the viral genome, we hybridized 35S B-77 RNA with normal duck DNA by either one of two techniques: when viral RNA was in excess and when DNA was in excess. The RNA of B-77 passaged only in gs-chf-CEF does not have homology with duck DNA. However, after several passages of B-77 through DEF the viral genome acquired duck specific RNA sequences. After 4 and 10 passages, B-77 RNA acquired 2.2 and 6.6%, respectively, complementarity to normal duck DNA. The duck specific RNA sequences were found to be covalently linked to the B-77 RNA genome. Also, the host specific sequences acquired by the virus appear to be from a region of the duck DNA which is repeated four to six times per cell. After 5 back passages in CEF some of the duck specific RNA sequences in the viral genome were lost.Avian oncornaviruses have the ability to be modified by the host through which they are passaged. Even Rous sarcoma virus (RSV) may have had to be adapted to induce tumors and replicate in chickens (1). After the isolation of chicken tumor no. 1 by Rous in 1911, the tumor cells could cause sarcomas only in chickens of the same strain as that in which the tumor originated and only by inoculation of large numbers of tumor cells. Subsequently, tumors could be induced in other strains of chickens but only after several more passages could sarcomas be induced by a cell-free filtrate. After more than 60 years of laboratory manipulations, RSV can now rapidly induce tumors in a wide host range including primates (2, 3). DuranReynals reported that after several passages in ducks, a duck adapted variant of RSV appeared (4). In addition to the ability to cause tumors in ducks, the duck-adapted virus had acquired new tissue affinities causing tumors in bone, skin, and digestive tract, whereas the original RSV did not. Furthermore, the duck-adapted virus had altered surface antigenic properties. A similar phenomenon has also been noted with RSV that has been rescued from transformed rat cells (5-12). These findings led Altaner and Temin (6) to suggest that the alteration of physical and biological properties in the virus may reflect recombination between viral and host genetic material. Host-induced alterations of murine sarcoma viruses have also been reported after passage in rat cells (13). Kirsten and Harvey sarcoma viruses appear to contain murine and rat oncornavirus specific RNA sequences but these sequences have Abbreviations: DEF, duck embryo fibroblasts; CEF, chicken embryo fibroblasts; RSV, Rous sarcoma virus; AMV, avian myeloblastosis virus; Cot, nucleotide concentration (mol/l) X time (sec); TCA, trichloroacetic acid.1031 not been shown to be covalently linked. Also, in those studies the DNA probe synthesized in vitro with reverse transcriptase may have contained cellular as well as viral sequences sin...

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supporting

confidence: 71%

Host induced alteration of avian sarcoma virus B-77 genome.

Shoyab¹,

Markham²,

Baluda³

1975

Proc. Natl. Acad. Sci. U.S.A.

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“…This rat 'variant' of B77 was shown to be antigenically modified and had increased transforming activity on rat cells [Altaner and Temin, 1970] and hamster cells [Svec et al, 1970]. Kryukova et al [1968Kryukova et al [ ,1970, Obukh and Kryukova [1969], and found that tumors are efficiently produced in adult mice if fibroblasts are first infected in vitro with CZ-RSV and then transferred to syngeneic animals. From such tumors 'var iants' again were obtained which showed high oncogenicity for mice and hamsters.…”

Section: The Src Gene Product and Cell Transformationmentioning

confidence: 99%

Rous Sarcoma Virus

Svoboda¹

1986

Intervirology

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Malignant and transforming activity of rous sarcoma virus. I. Malignant effect of rous sarcoma virus

Obukh

Kryukova

Biryulina

et al. 1969

Intl Journal of Cancer

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Cultures of cells from embryos of Af, C3H-Hg and (CBAT6T6 x Af)F, mice rapidly developed oncogenicity for syngeneic animals after exposure to RSV (Carr-Zilber strain). The tumours of mice were virogenic and contained the group-specific antigen of avian sarcoma-leukosis complex. However, when the infected cells became transformed in morphology, they lost their oncogenicity for syngeneic animals.The high oncogenicity of Af mouse embryonic cells infected with Carr-Zilber strain of RSV for the syngeneic animals was described in our previous communication (Kryukova et al., 1968).The tumours were virogenic, i.e. the introduction of the intact cells into chicks produced Rous sarcomas. After transplantation of these virogenic tumours into chicks and passing through chicks the resulting virus could induce tumours in mice. The transformed cultures appeared to be non-oncogenic for syngeneic Af mice. The further investigation of this phenomenon is described in this paper. MATERIAL AND METHODS Preparation of virus (Carr-Zilber strain)Virus-containing chicken tumour extract, diluted 1 :3 and centrifuged at 400 x g for 20 min, was injected intramuscularly into 7to 10-day-old White Leghorn chicks. Five or 6 days after injection the tumours were excised, pooled, homogenized, frozen in a dry-iceacetone mixture, and then stored at -30" C.

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Malignant and transforming activity of rous sarcoma virus. II. The study of variants of rous sarcoma virus isolated from mouse tumours

Abstract: Virus variants from Af mouse tumours induced by syngeneic cultured cells infected

Cited by 5 publications

References 3 publications

Host induced alteration of avian sarcoma virus B-77 genome.

Host induced alteration of avian sarcoma virus B-77 genome.

Rous Sarcoma Virus

Malignant and transforming activity of rous sarcoma virus. I. Malignant effect of rous sarcoma virus

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