Malignancies of the Uterine Corpus and Immunoreactivity Score of the DNA “Mismatch-Repair” Enzyme Human Mut-S-Homologon-2

Friedrich, Michael; Villena-Heinsen, C; Reitnauer, K; Schmidt, Werner; Tilgen, Wolfgang; Reichrath, Jörg

doi:10.1177/002215549904700112

Cited by 16 publications

(15 citation statements)

References 14 publications

(17 reference statements)

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“…To measure the neuritin immunoreactivity score (IRS), proliferative index (PI), apoptotic index (AI), and overall daily growth (ODG) in astrocytomas [11], especially in the most strongly stained tumor area of each section, 1,000 cells were observed in 5-10 adjacent high-powered fields at 9400 magnification. Neuritin protein expression was assessed semiquantitatively by calculating neuritin IRS according to the method described by Friedrich et al [12]. Because the staining of tumor cells is heterogeneous, SI was determined by the staining intensity of most cells.…”

Section: Evaluation Of Staining Resultsmentioning

confidence: 99%

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Neuritin expression and its relation with proliferation, apoptosis, and angiogenesis in human astrocytoma

et al. 2010

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Neuritin, a new member of the neurotrophic factor family, plays an important role in promoting neuronal survival, differentiation, function, and repair. However, whether neuritin is expressed in human astrocytoma and involved in their proliferation, apoptosis, and angiogenesis remains unclear. The expression of neuritin messenger RNA, protein and the relationship with proliferation, apoptosis, and angiogenesis were examined in human astrocytoma samples and three glioma cell lines by immunohistochemistry, Western blot, and quantitative real-time RT-PCR and so on. And neuritin immunoreactivity score (IRS), proliferative index (PI), apoptotic index (AI), overall daily growth (ODG), and microvessel density (MVD) in brain astrocytoma were measured. The results showed that neuritin was overexpressed in human astrocytoma samples, and the overexpression correlated positively with the malignancy of astrocytomas as reflected by changes in proliferation, apoptosis, and angiogenesis markers. In our study, we found neuritin is overexpressed in astrocytoma, which may be an important factor in tumorigenesis and progression of astrocytoma, and can be used as a target for biological therapy.

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Section: Evaluation Of Staining Resultsmentioning

confidence: 99%

“…And tumorigenesis of astrocytoma involves multiple factors, multiple gene mutations, and many signal transduction pathways, which is the role especially played by NTs [12]. However, the mechanisms are not fully understood at present.…”

Section: Discussionmentioning

confidence: 99%

Neuritin expression and its relation with proliferation, apoptosis, and angiogenesis in human astrocytoma

et al. 2010

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“…The IRS results from the multiplication of a staining intensity score (negative, 0; weak, 1; moderate, 2; strong, 3) and the percentage score of immunopositive cells (SI-negative, 0; weak, 1; moderate, 2; strong, 3). The immunoreactivity for Ki67 was evaluated and compared with other immunoreactivities with a scoring after Friedrich et al (9) that creates a ranking of the percentages of immunopositive cells (PP) (rank 0, 0%; 1, 1-10%; 2, 11-25%; 3, >25%).…”

Section: Methodsmentioning

confidence: 99%

“…Alterations of MMR protein expression have been detected in various gynecological tumors, i.e. in endometrial cancer (5,6), ovarian cancer (7,8), malignancies of the uterine corpus (9) and breast cancer (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemistry of proteins for DNA mismatch repair in correlation to prognostic factors of mammarian cancer

Köster¹,

Schröer²,

Fischer³

et al. 2007

Oncol Rep

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Abstract. Mutations in genes of the DNA mismatch repair system (MMR) are strongly linked to the development of hereditary non-polyposis colorectal cancer and play a significant role in sporadic cancer too. Besides the repair of chromosomal mismatches produced during replication, the MMR is the linkage of DNA mismatches to cell cycle control. Proteins of the MMR are necessary for the induction of apoptosis in response to non-tolerable amounts of DNA damage. We correlated the immunoreactivity of the MMR proteins hMSH2, hMLH1 and PMS2 to the immunoreaction of p53, the proliferation marker Ki67 and clinical prognosis factors such as tumor grading and staging, steroid receptor expression and hemangiosis carcinomatosa or lymphangiosis carcinomatosa in 200 samples from patients with diagnosed breast cancer. No correlation could be detected among the expression of the three MMR-proteins hMSH2, hMLH1 and PMS2. The expression of hMSH2 correlated positively with the expression of p53, with the appearance of distant metastases, low differentiation and the appearance of hemangiosis carcinomatosa and lymphangiosis carcinomatosa, while it negatively correlated with the expression of the estrogen receptor. No correlation was detected between hMLH1 or PMS2 and any of the investigated factors. The expression of hMSH2 seems to be related with predictors of an unfavorable course of disease in breast cancer.

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“…200 (for AI measurement) magnifications. According to the method described by Friedrich et al [21] , the EphA2 IRS was determined by semiquantitative assessment. The EphA2 IRS was obtained by multiplication of the values of the percentage of EphA2-positive cells (0, !…”

Section: Staining Interpretationmentioning

confidence: 99%

Expression of EphA2 in Human Astrocytic Tumors: Correlation with Pathologic Grade, Proliferation and Apoptosis

Li¹,

Wang

et al. 2007

Tumor Biol

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A high expression of EphA2 has been detected in many non-central nervous system tumors; however, the EphA2 expression in brain astrocytic tumors remains unclear. In this study, we investigated the expression of EphA2 mRNA and protein in 90 cases of human astrocytic tumors by reverse transcription polymerase chain reaction and immunohistochemistry, respectively. The proliferative index (PI) of tumor cells was evaluated by Ki-67 immunohistochemistry, and the apoptotic index (AI) was determined by TdT-mediated dUTP nick end labeling assay. The correlation between EphA2 expression, pathologic grade, proliferation and apoptosis of astrocytic tumors was further analyzed. The results showed that 47.8% of cases expressed EphA2 mRNA and 43.3% of cases expressed EphA2 protein. With increasing pathologic grade, the positive rate of EphA2 mRNA and protein, as well as the EphA2 immunoreactivity score (IRS), increased markedly. The PI in the EphA2-positive group was significantly higher than in the EphA2-negative group. In addition, the PI was positively correlated with EphA2 IRS. Although there was no significant difference between the AI in the EphA2-positive group and that in the EphA2-negative group, the AI was inversely correlated with EphA2 IRS. Therefore, EphA2 may be a new biomarker for astrocytic tumors. It may also affect the proliferation and apoptosis of tumor cells and be an attractive therapy target for astrocytic tumors.

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Malignancies of the Uterine Corpus and Immunoreactivity Score of the DNA “Mismatch-Repair” Enzyme Human Mut-S-Homologon-2

Cited by 16 publications

References 14 publications

Neuritin expression and its relation with proliferation, apoptosis, and angiogenesis in human astrocytoma

Neuritin expression and its relation with proliferation, apoptosis, and angiogenesis in human astrocytoma

Immunohistochemistry of proteins for DNA mismatch repair in correlation to prognostic factors of mammarian cancer

Expression of EphA2 in Human Astrocytic Tumors: Correlation with Pathologic Grade, Proliferation and Apoptosis

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