Maleic anhydride and acetylene plasma copolymer surfaces for SPR immunosensing

Makhneva, Ekaterina; Pastucha, Matěj; Obrusník, Adam; Horáčková, Veronika; Skládal, Petr; Zajı́čková, Lenka

doi:10.1007/s00216-019-01979-9

Cited by 17 publications

(6 citation statements)

References 32 publications

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“…From these results, experimental detection and quantification limits of 0.1 µg mL −1 and 0.4 µg mL −1 , respectively, of IgG in label-free conditions are inferred. This is a promising sensitivity which is in the range of other recent label-free optical approaches (Chen et al, 2018;Gatterdam et al, 2017;Juste-Dolz et al, 2018;Makhneva et al, 2019).…”

Section: Immunosensingmentioning

confidence: 93%

BIO bragg gratings on microfibers for label-free biosensing

Juste-Dolz

Delgado-Pinar

Avella-Oliver

et al. 2021

Biosensors and Bioelectronics

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Section: Immunosensingmentioning

confidence: 93%

BIO bragg gratings on microfibers for label-free biosensing

Juste-Dolz

Delgado-Pinar

Avella-Oliver

et al. 2021

Biosensors and Bioelectronics

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“…The developed SPR immunosensors had the same sensitivity as the conventionally used SAMs and CMD based immunosensors, but offered a better stability and regenerability. The level of response was larger than that in the case of SAMs based sensors, but smaller than in case of CMD based sensors . Such results can be explained by the fact that the surface area of SAMs is smaller than of the pp films, but CMD layers are a loose 3D network and have a much larger surface area .…”

Section: Discussionmentioning

confidence: 79%

Functional Plasma Polymerized Surfaces for Biosensing

Makhneva

Barillas

Pastucha

et al. 2020

ACS Appl. Mater. Interfaces

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The capabilities of biosensors for fast, economic, and user-friendly analysis of complex samples has led to the exploitation of analytical devices for detection, quantification, and monitoring of specific chemical species for various applications. For a sufficiently high surface reactivity toward the adopted bioreceptors, a thin functional layer is required to enable coupling of the target biomolecules and to provide good stability in the presence of a sample matrix. In this work, the generation of water-stable oxygen-rich plasma polymerized (pp) films deposited by atmospheric-pressure jet plasma for reliable immobilization of biomolecules is presented. Three types of pp films were developed and characterized. All of the obtained pp films were successfully used as a matrix layer in the SPR immunosensors, which provided excellent level of sensitivity, stability, and regenerability. The achieved results show that atmospheric pressure plasma-induced polymerization is a powerful alternative method for the preparation of matrix layers for a wide range of applications in the biological field.

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“…This methodology can be a clear alternative to commercial or selfmanufactured dextran chips or more recent techniques like carboxyl-rich plasma-polymerized films as no extensive reaction times and coupling steps have to be conducted prior to the experiments. [41,42] With our nanoparticle-mediated structures, measurements can be conducted "live" starting from bare gold.…”

Section: Discussionmentioning

confidence: 99%

Gold Albumin Sandwich Structures for Enhanced Biosensing Using Surface Plasmon Resonance

Rüger

Keusgen

Vornicescu

2021

Physica Status Solidi (a)

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The sensitivity of surface plasmon resonance (SPR) is limited by physico‐optical constraints like the quality of the optical elements, resolution of the optical detection element, and temperature control, but also by the surface area of the gold layer, which serves for interaction events. To increase the utilized sensing surface area, gold‐coated chips are loaded with gold nanoparticles (AuNPs). The nanoparticles are synthesized by two different methods and in different sizes. Experimental conditions are elaborated to bind AuNPs stabilized in citrate buffer directly to a SPR surface, which is coated with bovine serum albumin (BSA) prior to binding. The formed sandwich structures are functional without additional coupling steps and provide a time‐efficient tool for rapid and sensitive detection of various biomolecules. The AuNPs are successfully loaded with streptavidin (SaV). This allows convenient immobilization of all kinds of biotinylated molecules (e.g., enzymes, antibodies, etc.). The latter strategy has the advantage to circumvent the denaturation of proteins, which is caused by close contact to gold surfaces. Limit of detection and limit of quantification are found to be in the femtomolar range.

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Maleic anhydride and acetylene plasma copolymer surfaces for SPR immunosensing

Cited by 17 publications

References 32 publications

BIO bragg gratings on microfibers for label-free biosensing

BIO bragg gratings on microfibers for label-free biosensing

Functional Plasma Polymerized Surfaces for Biosensing

Gold Albumin Sandwich Structures for Enhanced Biosensing Using Surface Plasmon Resonance

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