Maleic acid treatment of biologically detoxified corn stover liquor

Kim, Dae-Hwan; Ximenes, Eduardo; Nichols, Nancy N.; Cao, Guangwen; Frazer, Sarah E.; Ladisch, Michael R.

doi:10.1016/j.biortech.2016.05.086

Cited by 25 publications

(32 citation statements)

References 35 publications

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“…In addition, the pretreatment of lignocellulosic biomass to increase access often results in the production of lignin-derived molecules (mainly phenolic acids) that inhibit enzyme activity and/or subsequent downstream processes such as microbial fermentation. The presence of lignin presents another hurdle for efficient enzymatic saccharification of biomass through non-productive binding of the enzymes [8,[11][12][13][14].Pretreatment principally solubilizes hemicellulose and lignin, and reveals inner cellulose molecules that are susceptible to being hydrolyzed by cellulolytic enzymes [15,16]. In addition, substrate particle size, cellulose crystallinity, and cellulose degree of polymerization are decreased during pretreatment, which results increased porosity and surface area that helps digestibility with cellulolytic enzymes [17][18][19][20].…”

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confidence: 99%

“…In order to alleviate the detrimental effects of lignin and lignin-derived inhibitors on biomass digestion, several different approaches have been pursued. Alriksson and colleagues examined the efficacy of in situ detoxification with reducing agents [29], others have employed activated charcoal [14,16], liquid-liquid extraction [30], lignin-blocking additives (bovine serum albumin or soybean protein) [31][32][33], biological detoxification [13,15] or genetic modification of the lignin [34][35][36][37]. These approaches attack the problem of recalcitrance due to lignin by reducing the concentrations of potential inhibitory molecules, by minimizing the non-productive adsorption of enzymes and/or by reducing concentration of lignin in the biomass to start with.…”

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Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse

et al. 2020

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Lignin contributes to the rigid structure of the plant cell wall and is partially responsible for the recalcitrance of lignocellulosic materials to enzymatic digestion. Overcoming this recalcitrance is one the most critical issues in a sugar-flat form process. This study addresses the effect of low lignin sugarcane bagasse on enzymatic hydrolysis after liquid hot water pretreatment at 190 °C and 20 min (severity factor: 3.95). The hydrolysis of bagasse from a sugarcane line selected for a relatively low lignin content, gave an 89.7% yield of cellulose conversion to glucose at 40 FPU/g glucan versus a 68.3% yield from a comparably treated bagasse from the high lignin bred line. A lower enzyme loading of 5 FPU/g glucan (equivalent to 3.2 FPU/g total solids) resulted in 31.4% and 21.9% conversion yields, respectively, for low and high lignin samples, suggesting the significance of lignin content in the saccharification process. Further increases in the enzymatic conversion of cellulose to glucose were achieved when the bagasse sample was pre-incubated with a lignin blocking agent, e.g., bovine serum albumin (50 mg BSA/g glucan) at 50 °C for 1 h prior to an actual saccharification. In this work, we have demonstrated that even relatively small differences in lignin content can result in considerably increased sugar production, which supports the dissimilarity of bagasse lignin content and its effects on cellulose digestibility. The increased glucose yields with the addition of BSA helped to decrease the inhibition of non-productive absorption of cellulose enzymes onto lignin and solid residual lignin fractions.

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Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse

et al. 2020

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“…The dried material was ground in a knife mill and the particle diameter <2 mm selected for the pretreatment. Corn stover was harvested from central Illinois, dried at room temperature to 5–8% moisture, and ground (Model 4 Wiley, Thomas Scientific, Swedesboro, NJ) to pass through a 2 mm screen (D. Kim et al, ).…”

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confidence: 99%

“…Pretreatments were based on using LHW conditions as described by Kohlmann, Westgate, Weil, and Ladisch (), and by D. Kim et al (), Y. Kim, Hendrickson, Mosier, and Ladisch (), Y. Kim, Kreke, Ko, and Ladisch (), and Y. Kim, Kreke, Mosier, and Ladisch (). Pretreatment liquid (800 ml) was obtained from sugarcane bagasse after LHW pretreatment in a 5 L reactor (Model 4580, Parr Instruments) using a 10% (wt/vol) solid loading and carried out at 195°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Inhibitor‐tolerant Komagataeibacter species could position the pretreatment liquid as a valuable resource for production of bacterial nanocellulose by directly converting, and thereby concentrating the glucose present at low concentration levels into an insoluble, readily recovered value‐added product (Zhang, Winestrand, Chen, et al, ; Zhang, Winestrand, Guo, et al, ; Zou et al, ). However, pretreatment liquid contains, in addition to dilute sugars (such as glucose and xylose), acetic acid and phenolic inhibitors in a range of lignocellulosic feedstocks (Table ; Cao et al, ; Cao, Ximenes, Nichols, Zhang, & Ladisch, ; D. Kim et al, ; Michelin, Ximenes, Polizeli, & Ladisch, ; Ximenes, Farinas, Kim, & Ladisch, ; Ximenes, Kim, Mosier, Dien, & Ladisch, , ). Hence, inhibitor tolerant bacteria are required to avoid costs of removing inhibitors (Guo et al, ), particularly phenolics, and would enable the generation of bacterial nanocellulose in the presence of inhibitors generated by liquid hot water (LHW) pretreatment.…”

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Adaptive laboratory evolution of nanocellulose‐producing bacterium

Vasconcellos

Farinas

Ximenes

et al. 2019

Biotech & Bioengineering

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Adaptive laboratory evolution through 12 rounds of culturing experiments of the nanocellulose-producing bacterium Komagataeibacter hansenii ATCC 23769 in a liquid fraction from hydrothermal pretreatment of corn stover resulted in a strain that resists inhibition by phenolics. The original strain generated nanocellulose from glucose in standard Hestrin and Schramm (HS) medium, but not from the glucose in pretreatment liquid. K. hansenii cultured in pretreatment liquid treated with activated charcoal to remove inhibitors also converted glucose to bacterial nanocellulose and used xylose as carbon source for growth. The properties of this cellulose were the same as nanocellulose generated from media specifically formulated for bacterial cellulose formation. However, attempts to directly utilize glucose proved unsuccessful due to the toxic character of the lignin-derived phenolics, and in particular, vanillan and ferulic acid.Adaptive laboratory evolution at increasing concentrations of pretreatment liquid from corn stover in HS medium resulted in a strain of K. hansenii that generated bacterial nanocellulose directly from pretreatment liquids of corn stover. The development of this adapted strain positions pretreatment liquid as a valuable resource since K. hansenii is able to convert and thereby concentrate a dilute form of glucose into an insoluble, readily recovered and value-added product-bacterial nanocellulose.

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Combining chemical flocculation and bacterial co‐culture of Cupriavidus taiwanensis and Ureibacillus thermosphaericus to detoxify a hardwood hemicelluloses hydrolysate and enable acetone–butanol–ethanol fermentation leading to butanol

Theiri

Chadjaa

Marinova

et al. 2018

Biotechnology Progress

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Butanol, a fuel with better characteristics than ethanol, can be produced via acetone–butanol–ethanol (ABE) fermentation using lignocellulosic biomass as a carbon source. However, many inhibitors present in the hydrolysate limit the yield of the fermentation process. In this work, a detoxification technology combining flocculation and biodetoxification within a bacterial co‐culture composed of Ureibacillus thermosphaericus and Cupriavidus taiwanensis is presented for the first time. Co‐culture‐based strategies to detoxify filtered and unfiltered hydrolysates have been investigated. The best results of detoxification were obtained for a two‐step approach combining flocculation to biodetoxification. This sequential process led to a final phenolic compounds concentration of 1.4 g/L, a value close to the minimum inhibitory level observed for flocculated hydrolysate (1.1 g/L). The generated hydrolysate was then fermented with Clostridium acetobutylicum ATCC 824 for 120 h. A final butanol production of 8 g/L was obtained, although the detoxified hydrolysate was diluted to reach 0.3 g/L of phenolics to ensure noninhibitory conditions. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2753, 2019.

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Maleic acid treatment of biologically detoxified corn stover liquor

Cited by 25 publications

References 35 publications

Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse

Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse

Adaptive laboratory evolution of nanocellulose‐producing bacterium

Combining chemical flocculation and bacterial co‐culture of Cupriavidus taiwanensis and Ureibacillus thermosphaericus to detoxify a hardwood hemicelluloses hydrolysate and enable acetone–butanol–ethanol fermentation leading to butanol

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