Male Sterility

Budar, Françoise; Delourme, Régine; Pelletier, Georges

doi:10.1007/978-3-662-06164-0_4

Cited by 12 publications

(8 citation statements)

References 104 publications

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“…To achieve complete stability of the Rfk1 gene, also exploited in practical breeding, the integration of the gene to the A genome chromosomes of turnip rape may be required. The Rfo gene, which is stably integrated in the oilseed rape C genome (Hu et al 2008;Feng et al 2009), is now used for hybrid seed production (Budar et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

et al. 2012

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In spring turnip rape (Brassica rapa L. spp. oleifera), the most promising F1 hybrid system would be the Ogu-INRA CMS/Rf system. A Kosena fertility restorer gene Rfk1, homolog of the Ogura restorer gene Rfo, was successfully transferred from oilseed rape into turnip rape and that restored the fertility in female lines carrying Ogura cms. The trait was, however, unstable in subsequent generations. The physical localization of the radish chromosomal region carrying the Rfk1 gene was investigated using genomic in situ hybridization (GISH) and bacterial artificial chromosome-fluorescence in situ hybridization (BAC-FISH) methods. The metaphase chromosomes were hybridized using radish DNA as the genomic probe and BAC64 probe, which is linked with Rfo gene. Both probes showed a signal in the chromosome spreads of the restorer line 4021-2 Rfk of turnip rape but not in the negative control line 4021B. The GISH analyses clearly showed that the turnip rape restorer plants were either monosomic (2n=2x=20+1R) or disomic (2n=2x=20+2R) addition lines with one or two copies of a single alien chromosome region originating from radish. In the BAC-FISH analysis, double dot signals were detected in subterminal parts of the radish chromosome arms showing that the fertility restorer gene Rfk1 was located in this additional radish chromosome. Detected disomic addition lines were found to be unstable for turnip rape hybrid production. Using the BAC-FISH analysis, weak signals were sometimes visible in two chromosomes of turnip rape and a homologous region of Rfk1 in chromosome 9 of the B. rapa A genome was verified with BLAST analysis. In the future, this homologous area in A genome could be substituted with radish chromosome area carrying the Rfk1 gene.

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Section: Discussionmentioning

confidence: 99%

Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

et al. 2012

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“…Selection of the resulting recombinant mitochondrial genomes can be used to improve systems in terms of flower morphology, attractiveness to insects, and restoration. This concept was initially developed for N. tabacum in the mid-1970s, but has since been applied to several Brassica species, leading to the development of exploitable systems at the start of the 1990s [28].…”

Section: Protoplast Fusionmentioning

confidence: 99%

The molecular biology of cytoplasmically inherited male sterility and prospects for its engineering

Pelletier¹,

Budar²

2007

Current Opinion in Biotechnology

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“…For Brassica crops, since a sterility inducing cytoplasm identified in a wild population of Raphanus sativus (Ogura CMS) was introduced into B. napus and B. oleracea (Bannerot et al, 1974), a spectrum of alloplasmic CMS lines of diverse origins have been obtained by combining the cytoplasm of Brassica coenospecies with crop nuclei, particularly in B. juncea (see reviews by Delourme and Budar, 1999; Budar et al, 2004; Prakash et al, 2009; Yamagishi and Bhat, 2014). More recently, a novel CMS system in B. juncea incorporating the cytoplasm of B. fruticulosa was developed (Atri et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A Novel Cytoplasmic Male Sterility in Brassica napus (inap CMS) with Carpelloid Stamens via Protoplast Fusion with Chinese Woad

Kang

Wang

et al. 2017

Front. Plant Sci.

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A novel cytoplasmic male sterility (CMS) in Brassica napus (inap CMS) was selected from the somatic hybrid with Isatis indigotica (Chinese woad) by recurrent backcrossing. The male sterility was caused by the conversion of tetradynamous stamens into carpelloid structures with stigmatoid tissues at their tips and ovule-like tissues in the margins, and the two shorter stamens into filaments without anthers. The feminized development of the stamens resulted in the complete lack of pollen grains, which was stable in different years and environments. The pistils of inap CMS displayed normal morphology and good seed-set after pollinated by B. napus. Histological sections showed that the developmental alteration of the stamens initiated at the stage of stamen primordium differentiation. AFLP analysis of the nuclear genomic composition with 23 pairs of selective primers detected no woad DNA bands in inap CMS. Twenty out of 25 mitochondrial genes originated from I. indigotica, except for cox2-2 which was the recombinant between cox2 from woad and cox2-2 from rapeseed. The novel cox2-2 was transcribed in flower buds of inap CMS weakly and comparatively with the fertile B. napus addition line Me harboring one particular woad chromosome. The restorers of other autoplasmic and alloplasmic CMS systems in rapeseed failed to restore the fertility of inap CMS and the screening of B. napus wide resources found no fertility restoration variety, showing its distinct origin and the related mechanism of sterility. The reasons for the mitochondrial rearrangements and the breeding of the restorer for the novel CMS system were discussed.

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Male Sterility

Cited by 12 publications

References 104 publications

Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

Size and location of radish chromosome regions carrying the fertility restorer Rfk1 gene in spring turnip rape

The molecular biology of cytoplasmically inherited male sterility and prospects for its engineering

A Novel Cytoplasmic Male Sterility in Brassica napus (inap CMS) with Carpelloid Stamens via Protoplast Fusion with Chinese Woad

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