Male Sex Preselection: Swim-Up Technique and Insemination of Women After Ovulation Induction

Check, Jerome H.; Shanis, Bonnie S.; S, Cooper; Bollendorf, A.

doi:10.3109/01485018908986839

Cited by 25 publications

(17 citation statements)

References 5 publications

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“…Y bearing spermatozoa are reported to swim faster than X bearing spermatozoa due its smaller size. Success rate in this method was reported to be 81% (Check et al, 1989). …”

Section: Swim Up Proceduresmentioning

confidence: 99%

Sexing of Spermatozoa in Farm Animals: a Mini Review

Prakash¹

2014

Adv. Anim. Vet. Sci.

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Livestock farmers always have a wish for producing young ones of desired sex. Among the several techniques available, use of sexed semen for artificial insemination is recognized as more pragmatic and easy way to pre -select the sex of the offspring. Selective use of sexed semen in breeding will not only increase the genetic progress from the daughter -dam path but would also help in producing good male germplasm from elite bulls for future breeding. Several attempts have been made, elsewhere in the globe, to develop methods that efficiently separate bovine semen into fractions containing higher concentrations of X -or Y -bearing sperm. These technologies include sex specific antibodies, centrifugation and flow cytometry. Of these attempts, the only method proven to be commercially viable is flow cytometry. However, sorting pressure, speed, electrical deviation, laser radiation all leads to membrane alteration and pre -capacitation like changes in the sorted sperm leading to reduced fertility. Despite these limitations, production of sexed semen usually followed by cryopreservation is being used commercially for cattle production. Development of the instrument for increasing the sorting rate and also purity of sorting without affecting the viability and fertility is still an active area of research. The aim of this review is to update the readers with the recent developments in sexing of spermatozoa with special reference to farm animals.All copyrights reserved to Nexus® academic publishers

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“…Y bearing spermatozoa are reported to swim faster than X bearing spermatozoa due its smaller size. Success rate in this method was reported to be 81% (Check et al, 1989). …”

Section: Swim Up Proceduresmentioning

confidence: 99%

Sexing of Spermatozoa in Farm Animals: a Mini Review

Prakash¹

2014

Adv. Anim. Vet. Sci.

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“…Pemisahan spermatozoa dengan swim up didasarkan atas perbedaan kecepatan renang spermatozoa berkromosom X dengan spermatozoa berkromosom Y ke luar dari pellet menuju ke permukaan media (Yuliani, 2000;Boediono et al, 2007). Spermatozoa berkromosom Y mempunyai kemampuan renang lebih cepat dibandingkan spermatozoa berkromosom X. Telah terbukti bahwa spermatozoa yang dipisahkan dengan swim up diperoleh populasi spermatozoa berkromosom Y lebih banyak secara sangat nyata pada lapisan atas dibandingkan dengan populasi spermatozoa berkromosom X. Sebaliknya populasi spermatozoa berkromosom Y yang diperoleh pada lapisan bawah lebih sedikit secara sangat nyata dibandingkan dengan populasi spermatozoa berkromosom X ( Check et al, 1998;Yuliani, 2000). Tingkat keberhasilan pemisahan spermatozoa pembawa kromosom X dan Y pada metode swim up sangat dipengaruhi oleh lama waktu swim up (De Jounge et al, 1997;Claassens et al, 1998 …”

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Rasio Jenis Kelamin Kelahiran Anak Kambing Peranakan Ettawa (PE) Hasil Inseminasi Buatan Menggunakan Spermatozoa Swim Up

2014

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Laboratorium embriologi dan histologi Fakultas Kedokteran Hewan Universitas Syiah KualaThe aim of this research was to determine the ratio of birth sex of ettawa crossbreed goat by artificial insemination using spermatozoa swim up. This research was at Reproduction Laboratory Faculty of Veterinary medicine Syiah Kuala university and Livestock Group SMD, Bener Meriah Regency. Fifteen female goats with age 8-24 months where used in this study and divided into 3 treatment groups (P0, P1, P2) and 5 replications of each. The first group (P0) inseminated with sperm without swim up; P1 and P2 inseminated with sperm which swim up for 5 minutes (P1) and 10 minutes (P2). The result of conception rates observation and sex ratio of children born tabulated into tables. The data was analyzed by using analysis of variance (ANOVA) one way pattern followed by the multiple test Duncan. The results showed that the conception rate and birth sex ratio of male born had significantly different (P<0,05). The segregation of sperm which swim up increased the conception rate and birth sex ratio of male born.

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“…Rawlins et al [8] found that pregnancies achieved by in vitro fertilization and IUI with spermatozoa prepared by the swim-up method resulted in 10 males delivered versus 1 female (90.9%). Check et al [9] reported 86% male deliveries after swim-up separation followed by insemination and the same high proportion of males born to women treated with ovulation-inducing drugs such as clomiphene citrate (CC).…”

Section: Introductionmentioning

confidence: 99%

“…Discontinuous albumin density gradients presented by Ericsson et al [5], Sephadex columns [6], discontinuous Percoll gradients [7] and swim-up procedures have all been widely used and researched. Two specific studies by Rawlins et al [8] and Check et al [9] used the swim-up technique to increase the percentage of Y-bearing spermatozoa and thus the percentage of male offspring after intrauterine insemination (IUI). Rawlins et al [8] found that pregnancies achieved by in vitro fertilization and IUI with spermatozoa prepared by the swim-up method resulted in 10 males delivered versus 1 female (90.9%).…”

Section: Introductionmentioning

confidence: 99%

A Controlled Study for Gender Selection Using Swim-Up Separation

Khatamee

Horn²,

Weseley³

et al. 1999

Gynecol Obstet Invest

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Objective: To evaluate the success for gender selection using a sample of semen separated by a modified swim-up technique. Design: We retrospectively compared the gender outcome of two treatments (A and B) for either a male or female offspring with those who conceived spontaneously. Setting: Private practice of one author (M.A.K.). Patients, Participants: The treatment groups consisted of 52 total pregnancies for couples who conceived by the separation technique. Of these 52 participants, 15 desired a female offspring and were placed into treatment A and 37 desired a male offspring and were placed into treatment B. The control groups consisted of 162 women who were presented with initial consultation for gender selection and conceived spontaneously. Control group A consisted of 80 women who initially chose a female offspring, and control group B consisted of 82 participants who initially chose a male. Interventions: In treatment group A, one timed intrauterine insemination (IUI) was carried out with the bottom 0.5 ml of the separated semen on cycle days 12–14, when the follicle was 18–22 mm. Patients in this group were also administered clomiphene citrate and human chorionic gonadotropin. In treatment group B, one timed IUI was done with the top 0.5 ml of the separated semen, when the follicle was 18–22 mm. Main Outcome Measure: The gender outcome of the pregnancies of two treatment and control groups was evaluated based on the known desired gender. Results: The success rate for conceiving a female child after intervention (treatment group A) was 86.7% effective (p = 0.002) as compared to the control group A. Couples seeking a male child (treatment group B) were 89.2% effective (p = 0.0002) as compared to the control group B. Conclusions: This study reveals that the modified swim-up method with additional monitoring results in statistically significant gender preselection.

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Male Sex Preselection: Swim-Up Technique and Insemination of Women After Ovulation Induction

Cited by 25 publications

References 5 publications

Sexing of Spermatozoa in Farm Animals: a Mini Review

Sexing of Spermatozoa in Farm Animals: a Mini Review

Rasio Jenis Kelamin Kelahiran Anak Kambing Peranakan Ettawa (PE) Hasil Inseminasi Buatan Menggunakan Spermatozoa Swim Up

A Controlled Study for Gender Selection Using Swim-Up Separation

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