Male reproductive toxicity of CrVI: In-utero exposure to CrVI at the critical window of testis differentiation represses the expression of Sertoli cell tight junction proteins and hormone receptors in adult F 1 progeny rats

Kumar, K. Suresh; Aruldhas, M. M.; Banu, Sheerin L.; Sadasivam, Balaji; Ganapathy, Vengatesh; Ganesh, Karthik M.; Navaneethabalakrishnan, Shobana; Navin, Apurva; Prakash, Seppan; Venkatachalam, Sankar; Stanley, Jone A.; Ilangovan, R.; Banu, Sakhila K.; Akbarsha, Mohammad Abdulkader

doi:10.1016/j.reprotox.2017.02.007

Cited by 27 publications

(19 citation statements)

References 96 publications

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“…During the intracellular reduction process of CrVI to CrIII, a spectrum of reactive oxygen species (ROS) (ie, superoxide [O2•À], hydrogen peroxide [H2O2], and hydroxyl radical [•OH]) is produced, with the depletion of antioxidant enzymes culminating in increased oxidative stress (Standeven and Wetterhahn, 1991). We have identified oxidative stress as one of the major pathways for CrVI-induced male (Kumar et al, 2017) and female reproductive toxicity (Banu et al, 2016;Stanley et al, 2013Stanley et al, , 2014) in a rat model. However, there is no report on the impact of Cr on placental oxidative stress in the human.…”

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confidence: 99%

Sexually Dimorphic Impact of Chromium Accumulation on Human Placental Oxidative Stress and Apoptosis

Banu

Stanley

Taylor

et al. 2017

Toxicological Sciences

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Environmental contamination with hexavalent chromium (CrVI) is a growing problem both in the United States and developing countries. Hexavalent chromium is widely used in numerous industries. Environmental exposure to CrVI adversely affects pregnancy outcomes and subsequent health of 2 generations, resulting in higher pregnancy loss, spontaneous abortion and low birth rate. Pregnant women exposed to CrVI through occupational settings experience increased risk of spontaneous abortion, stillbirth, preterm birth, and neonatal death. Children of the CrVI exposed women experience respiratory problems, perinatal jaundice, and increased birth defects. Because placental dysfunction may have a role in such adverse pregnancy outcome, we tested the hypothesis that environmental Cr exposure in pregnant women results in Cr accumulation in the human placenta, which could increase placental oxidative stress by disrupting antioxidant machinery and inducing apoptosis. Studies using frozen, deidentified human term placenta samples indicated that: (1) Cr accumulates in human term placenta tissues and (2) increase in Cr accumulation is positively correlated with oxidative stress and apoptotic markers, and altered antioxidants levels. Interestingly, there was a sexual dimorphism in the correlation between Cr accumulation and oxidative stress, and expression of apoptotic and antioxidant markers. Mechanistic in vitro studies using human trophoblast cells BeWo confirmed the detrimental effects of Cr in altering antioxidant genes. For the first time, this study provides evidence in support of a positive correlation between Cr accumulation in the human placenta and accelerated oxidative stress, with a gender bias toward the male sex.

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confidence: 99%

Sexually Dimorphic Impact of Chromium Accumulation on Human Placental Oxidative Stress and Apoptosis

Banu

Stanley

Taylor

et al. 2017

Toxicological Sciences

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“…Chromium exposure has been associated with these pathological changes which may be a cause of impaired spermatogenesis. Additionally, chromium can cross the blood-testis barrier and effect sperm development, maturation, and sperm motility (Kumar et al 2017). We observed that sperm motility was more affected than any other parameters.…”

Section: Discussionmentioning

confidence: 68%

Correlation between urinary chromium level and semen quality in men attending an andrology laboratory

Pokhrel

Shi

Wang

et al. 2020

Environ Sci Pollut Res

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Chromium is an essential element that is required for the normal physiology but can be toxic to humans above a certain level. In spite of growing interest in research on chromium exposure to human health consensus about its effect on human, semen quality has not been achieved. The aim of the present study is to evaluate the impact of chromium exposure on semen parameters. A total of 760 males attending andrology laboratory of Tongji Hospital, Wuhan, for routine semen analysis were enrolled and requested to provide semen and urine samples. The urine level of chromium was evaluated using inductively coupled plasma mass spectrometry (ICP-MS), and computer-assisted semen analysis (CASA) was applied to examine semen parameters. Associations between semen parameters and urinary chromium were analyzed by means of multivariate linear regression analysis. Multivariate analysis showed a negative association between the urinary concentrations of chromium and progressive motility (β = − 0.014, p = 0.040) and total motility (β = − 1.077, p = 0.048), while other semen parameters did not show any statistically significant changes. Urinary chromium could influence semen quality parameters and impair male fecundity.

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“…Gut Histology: Cryosections of the ileum were stained for Hematoxylin and Eosin (H&E) as previously described (Kumar et al, 2017). Sections were visualized and photographed with the FSX100 Cell Imaging System (Olympus) at 10X magnification.…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemistry: Immunofluorescence for Zonula Occludens (ZO-1) was performed as previously described (Kumar et al, 2017). Briefly, a portion of the ileum was embedded in Cryo-OCT compound (Leica Microsystems, Buffalo Grove, IL, USA) and frozen in liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

Sex Differences in Stroke Outcome Are Associated With Constitutive Gut Dysbiosis and Stroke-induced Gut Permeability

El-Hakim

Mani

Eldouh

et al. 2020

Preprint

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Background: Sex differences in experimental stroke are well documented, such that adult males show worse outcomes compared to adult females, including greater infarct volume, increased stroke-induced mortality and more severe sensory-motor impairment. Based on recent evidence that gut dysbiosis may be an early response to stroke, the present study tested the hypothesis that in the acute phase, stroke will result in greater permeability of the gut blood barrier and gut dysbiosis in males as compared to females. Method: Male and female Sprague Dawley rats (5-7 months of age) were subject to endothelin (ET)-1-induced middle cerebral artery occlusion (MCAo). Fecal samples, blood draws and sensory motor tests were conducted pre and 2d after MCAo. Fecal samples were analyzed for 16s sequencing and short chain fatty acids (SCFAs). Gut permeability was assessed in serum samples using biomarkers of gut permeability as well as functional assays using size-graded dextrans. Results: We confirmed stroke-induced sex differences, including greater mortality and sensory motor deficit in males as compared to age-matched female rats. Remarkably, fecal 16s sequencing showed greater bacterial diversity in females at baseline (prior to stroke) while 2 days after stroke, these measures were similar between the sexes. In contrast, fecal levels of short chain fatty acids (SCFAs) which are usually beneficial, were higher in males. MCAo-induced gut permeability was much worse in males as compared to females, as indicated by histological analysis, biochemical markers in serum, and serum measurement of fluorescent-labeled dextrans following oral gavage. Additionally, males had higher serum levels of proinflammatory cytokines IL-17A, MCP-1, IL-5 and EGF compared to females after stroke. Predictive modeling indicated that markers of gut permeability were associated with stroke-induced sensory-motor impairment. Conclusions: Poor stroke outcomes in adult males is mirrored by increased gut permeability in this group. Additionally, these data suggest that constitutive sex differences in the diversity and richness of gut microbial communities may predispose better functional outcomes after stroke in females, and support the idea that preventative modification of the gut microbiome may reduce the risk for stroke in vulnerable populations such as the elderly or those with co-morbid conditions.

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Male reproductive toxicity of CrVI: In-utero exposure to CrVI at the critical window of testis differentiation represses the expression of Sertoli cell tight junction proteins and hormone receptors in adult F 1 progeny rats

Cited by 27 publications

References 96 publications

Sexually Dimorphic Impact of Chromium Accumulation on Human Placental Oxidative Stress and Apoptosis

Sexually Dimorphic Impact of Chromium Accumulation on Human Placental Oxidative Stress and Apoptosis

Correlation between urinary chromium level and semen quality in men attending an andrology laboratory

Sex Differences in Stroke Outcome Are Associated With Constitutive Gut Dysbiosis and Stroke-induced Gut Permeability

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