Male Recombination in a Genetic Sexing Strain of Ceratitis capitata (Diptera: Tephritidae) and Its Effect on Stability

Busch-Petersen, E.

doi:10.1093/aesa/82.6.778

Cited by 12 publications

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“…Such recombination may separate one or more of the mutations from the male-determining region of the Y chromosome. If the products of such recombination enjoy a selective advantage in rearing colonies, linkage of the critical mutation to sex can rapidly disappear (Busch-Petersen, 1989;Foster et at, 1980a;Hooper el at, 1987;Saul, 1984). In field trials this has contributed to serious genetic deterioration of release strains (Foster et a!., 1985;Hooper eta!., 1987).…”

Section: Introductionmentioning

confidence: 99%

Male crossing over and genetic sexing systems in the Australian sheep blowfly Lucilia cuprina

1991

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Field-female killing (FK) systems based on deleterious mutations arid Y-autosome translocations are being evaluated for genetic control of the Australian sheep blowfly, Lad/ia cuprina. Experience during field trials has shown that mass-reared colonies of FK strains are subject to genetic deterioration, caused mainly by genetic recombination in males. A previous study found higher male recombination frequencies in two Y-linked translocation strains than in chromosomally normal males. However, the results of the present study indicate that breakage of the Y chromosome is neither sufficient nor necessary for increased levels of male recombination. The frequency of male recombination appears to be unrelated to the presence of specific chromosome rearrangements.

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Section: Introductionmentioning

confidence: 99%

Male crossing over and genetic sexing systems in the Australian sheep blowfly Lucilia cuprina

1991

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“…Recent studies have revealed the presence of low levels of male recombination in several species of cyclorrhaphous Diptera, including M. domestica (Lester et al, 1979), L. cuprina (Whitten, 1979) C. capitata (Busch-Petersen, 1989a;Rossler, 1982aRossler, , 1982bRossler, , 1985 and even Drosophila melanogaster Meigen (Diptera: Drosophilidae) (Hiraizumi, 1971) in which it was thought to be absent. The presence of such recombination in genetic sexing strains will effectively sever the link between the selectable/allele ( fig.…”

Section: Discussionmentioning

confidence: 99%

“…When choosing a genetic sexing strain it is important to ensure that the frequency of male recombination is zero or extremely low. Based on past experience with genetic sexing strains in C. capitata (Busch-Petersen, 1989a;Busch-Petersen & Kafu, 1989;Hooper et al, 1987;Rossler, 1982aRossler, , 1982bRossler, , 1985 it appears that some male recombination is almost inevitable. If this is indeed the case, then steps must be taken to ensure a reduction in the frequency of effective male recombination in the relevant section of the chromosome, either by selecting strains with a minimum distance between the F allele and the translocation breakpoint (see Kerremans & Busch-Petersen, 1990), or by inducing a chromosomal inversion around this area (e.g., Kaiser et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

“…Although no case of reversion of the Y-autosome translocation has been observed in unstable genetic sexing strains of C. capitata (Busch-Petersen, 1989a), Foster et al (1980) reported such reversion to be partially responsible for the high level of instability observed in a genetically sexed strain of L. cuprina. We therefore included this potential mechanism of strain instability in our simulation programme.…”

Section: Male Recombination With Reversion Of the Translocation To Thmentioning

confidence: 99%

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Simulation of instability in genetic sexing strains: effects of male recombination in association with other biological parameters

Busch-Petersen¹,

Baumgartner²

1991

Bull. Entomol. Res.

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Genetic systems have been developed in several insect species for separating males and females prior to releasing sterilized males in pest control programmes using the sterile insect technique. The systems generally depend on translocating a readily selectable gene onto the Y chromosome. A potential source of instability in such a system is genetic recombination in the male. Although such recombination was originally thought to be absent in most cyclorrhaphous Diptera, low levels have recently been found. We have developed a computer model which simulates the progression of instability in the presence of male recombination, which can be used to assess the influence of rate of recombination in combination with a range of associated genetic and biological parameters. Male recombination alone or fitness of the Y-linked translocation were found to contribute relatively little to the rate of progression of instability. By contrast reduced fitness or mating competitiveness associated with the selectable gene had a strong effect. The sex ratio and the ratio of carriers to non-carriers of the selectable gene showed patterns characteristic of the parameters modelled. The relevance of such data to the development of suitable strains for genetic sex-separation and the replacement of strains under mass rearing conditions are discussed.

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“…The occasional recombinants observed have the genotypes Y-wp/wp (white males) or wp/wp (brown females) (Busch-Petersen, 1989). …”

Section: Introductionmentioning

confidence: 97%

Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

et al. 1993

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The frequency of male recombination (r) between the translocation breakpoint and the wp + gene, and the selection coefficient (s) against wp/ wp, in the genetic sexing strain T : Y( wp + )30C of the Mediterranean fruit fly Ceratitis capitata (Wied.) were measured in population experiments over 11 generations. The population was 50 females and 50 males in the first generation, after which 150 pupae were used to start each subsequent generation. A computer program was used to estimate values of r and s. The best fit to the observed data was obtained when r= 0.14 0.04 per cent and s= 26.0 2.7 per cent. Using these estimated values, predictions were made on the frequency of brown females and white males for up to 100 generations. The frequency of white males was predicted never to exceed 0.5 per cent, to be reached after 25 generations, while the frequency of brown females was expected to exceed 33 per cent after 25 generations. Published data on the mass reared T:Y(wp)30C strain, maintained with a population size of 240,000 adult flies, were subjected to the same analysis, indicating a higher value of s (38.0 3.2-52.0 0.3 per cent) under these conditions, but a similar value of recombination. Again, the frequency of white males was predicted never to exceed 0.5 per cent as a result of recombination. When analysing published data on T : Y( wp ÷ ) 101, another genetic sexing strain, a lower stability was indicated, i.e. a more rapid generation of brown females and white males than in T : Y( wp + )30C. No suitable values of s and r could be calculated from the T : Y( wp ÷ )101 data. It is concluded that outside contamination had probably occurred in the latter strain during both periods when it was mass reared.

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Male Recombination in a Genetic Sexing Strain of Ceratitis capitata (Diptera: Tephritidae) and Its Effect on Stability

Cited by 12 publications

References 0 publications

Male crossing over and genetic sexing systems in the Australian sheep blowfly Lucilia cuprina

Male crossing over and genetic sexing systems in the Australian sheep blowfly Lucilia cuprina

Simulation of instability in genetic sexing strains: effects of male recombination in association with other biological parameters

Spontaneous recombination between wp+ and the translocation breakpoint in the T:Y(wp+ )30C genetic sexing strain of Ceratitis capitata (Wied.)

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