Male meiosis and behaviour of sex chromosomes in different populations of Rumex acetosa L. from the Western Himalayas, India

Farooq, Umer; Lovleen,; Saggoo, Manjit Inder Singh

doi:10.1007/s00606-013-0881-z

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…It has been suggested many times that INM can cause aneuploid-or unreduced-pollen formation (Farooq et al, 2014;Fakhri et al, 2016;Djafri-Bouallag et al, 2019). In this context, nucleus-nucleus contacts observed in tobacco meiocytes look quite intriguing, and it can be assumed that they result in changes of the chromatin amount in nuclei of both donor and recipient cells.…”

Section: The Micronuclei Formation Is Not the Main Consequence Of Inm In Tobaccomentioning

confidence: 99%

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Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes

et al. 2021

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Serial block-face scanning electron microscopy (SBF-SEM) was used here to study tobacco male meiosis. Three-dimensional ultrastructural analyses revealed that intercellular nuclear migration (INM) occurs in 90–100% of tobacco meiocytes. At the very beginning of meiosis, every meiocyte connected with neighboring cells by more than 100 channels was capable of INM. At leptotene and zygotene, the nucleus in most tobacco meiocytes approached the cell wall and formed nuclear protuberances (NPs) that crossed the cell wall through the channels and extended into the cytoplasm of a neighboring cell. The separation of NPs from the migrating nuclei and micronuclei formation were not observed. In some cases, the NPs and nuclei of neighboring cells appeared apposed to each other, and the gap between their nuclear membranes became invisible. At pachytene, NPs retracted into their own cells. After that, the INM stopped. We consider INM a normal part of tobacco meiosis, but the reason for such behavior of nuclei is unclear. The results obtained by SBF-SEM suggest that there are still many unexplored features of plant meiosis hidden by limitations of common types of microscopy and that SBF-SEM can turn over a new leaf in plant meiosis research.

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Section: The Micronuclei Formation Is Not the Main Consequence Of Inm In Tobaccomentioning

confidence: 99%

“…Thus, this phenomenon is simply ignored in many works on plant meiosis. On the other hand, some researchers consider INM a process that leads to aneuploid-or unreduced-pollen formation (Farooq et al, 2014;Fakhri et al, 2016;Djafri-Bouallag et al, 2019). This point of view is not supported by sufficient experimental evidence.…”

Section: Introductionmentioning

confidence: 96%

Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes

et al. 2021

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“…Thus, migrated chromosomes (or their fragments carrying centromeric regions) can with a certain probability enter the daughter nuclei and contribute to the changes in the karyotype of formed gametes. Formation of aneuploid and unreduced pollen in plants with a high rate of cytomixis has been repeatedly demonstrated for different species including tobacco (Lavia et al 2011;Pécrix et al 2011;Farooq and Lovleen 2014;Fakhri et al 2016); however, there are still not enough of experimental data that confirm this hypothesis. The interaction of migrated chromatin with the spindle in the recipient cell, which we described, favors this hypothesis.…”

Section: An Mt-organizing Activity Of Migrated Chromatin Suggests Its Contribution To the Change In Cell Karyotypementioning

confidence: 99%

Analysis of cytoskeleton in the cells involved in cytomixis: the migrated chromatin displays an MT-organizing activity and can interact with the spindle

2019

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The structure of cytoskeleton in the tobacco male meiocytes involved in the migration of nuclei between cells (cytomixis) is studied. The tubulin and actin components of cytoskeleton are examined using specific antibodies and phalloidin. The presence of microtubules and actin filaments inside cytomictic channels directly when the nuclei migrate through these channels is demonstrated for the first time. The actin and tubulin cytoskeleton is shown to retain its reticular structure in early prophase I before the beginning of cytomixis, during nuclear migration, and after completion of this process and emergence of micronuclei in the cytoplasm of recipient cells. It is demonstrated that if migration takes place in late prophase I, the nucleus leaves the perinuclear tubulin cage, encompassing it at this stage. After migration, the cytomictic micronuclei in recipient cell reside beyond the perinuclear tubulin cage in both meiotic prophase I and II. A microtubule-organizing activity of the migrated chromatin in meta-telophase I and II is demonstrated for the first time as well as the ability of this chromatin to form independent minispindles and radial microtubule arrays and contact the spindle of recipient cell. The role of cytomixis in production of aneuploid and unreduced pollen is discussed.

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“…He also carried out the biochemical and molecular analysis of selected cytotypes for Chenopodium album and Rumex nepalensis. Farooq, Lovleen, et al (2013) investigated meiosis and behaviour of sex chromosomes in different populations of Rumex acetosa L. from the Kashmir and other parts of Western Himalaya. Farooq and Saggoo (2014) carried out cytomorphological investigations in Oxyria digyna Hil on a population basis from high altitudes.…”

Section: The Period 2006-2015mentioning

confidence: 99%

Cyto-genetic diversity with special reference to medicinal plants of the Kashmir Himalaya – a review

Siddique

Jeelani

2015

Caryologia

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The present paper reviews research conducted on the cytogenetics of the plants of the Kashmir Himalaya. The review discloses that out of the total c.550 cytologically determined plants, there are c.190 genera and c.55 families of angiosperms. Among the cytologically determined species, c.50 species have been reported for the first time at global level along with varied chromosomal reports for c.110 species from the Kashmir Himalaya. The genus Jaeschkea has also been cytologically determined for the first time worldwide from the Kashmir Himalaya. The chromosome numbers in these species vary from 2n = 10 to 2n = 120 with frequency of 2n = 18 (13.73%), 2n = 16 (13.46%), 2n = 14 (7.96%) and 2n = 32 (7.41%). The reported base numbers of x = 7, x = 8, x = 9 are common ones. Most of the genera are polybasic, followed by monobasic and dibasics while tribasic taxa are least represented in the Kashmir Himalaya. New base numbers have been reported for Astragalus (x = 6), Cheiranthus (x = 6), Gypsophila (x = 13), Meconopsis (x = 7) and Saussurea (x = 18) from this region. In all, nearly 65% species are diploids and the rest (35%) are polyploids existing at various polyploid levels, with a preponderance of tetraploids. These plants depict significant level of intraspecific variability not only in chromosome numbers but also for meiotic behaviour and morphology. The knowledge of intraspecific variability of these species point out the existence of genetic diversities. Additionally, it has been observed that molecular marker studies are restricted to few plant species in the geographically diverse the Kashmir Himalaya.

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Male meiosis and behaviour of sex chromosomes in different populations of Rumex acetosa L. from the Western Himalayas, India

Cited by 9 publications

References 19 publications

Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes

Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes

Analysis of cytoskeleton in the cells involved in cytomixis: the migrated chromatin displays an MT-organizing activity and can interact with the spindle

Cyto-genetic diversity with special reference to medicinal plants of the Kashmir Himalaya – a review

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