Male Gametophyte Development

McCormick, Sheila

doi:10.2307/3869779

Cited by 327 publications

(322 citation statements)

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“…Pollen expression type was determined based on the maximum expression value (MaxP) from the four pollen stages (uninucleate microspore, bicellular pollen, tricellular pollen, and mature pollen) to the maximum expression value (MaxS) from seven sporophytic tissues (cotyledon, leaves, petiole, stems, roots, root hair zone, and suspension cell cultures), or the value of MaxP/MaxS according to the pollen transcriptome dataset (Honys and Twell 2004). Late pollen genes are defined as those expressed after microspore mitosis during male gametophyte development and which continue to accumulate during pollen maturation (McCormick 1993).…”

Section: Plant Materialsmentioning

confidence: 99%

“…From the 239 down regulated genes in the Ms-cd1 mutant (141 Pollen Specific, 78 Pollen Preferential and 19 Pollen Expressed; Honys and Twell (2004)), 235 have the highest expression at late pollen stages after microspore mitosis during male gametophyte development (McCormick 1993). Only four genes (AT4G28700, AT1G73070, AT4G09740 and AT4G14780) down regulated in Ms-cd1 anthers were early expressed in the uninucleate microspore stage and repressed after pollen mitosis II (PMII) in the pollen transcriptomic dataset (Honys and Twell 2004).…”

Section: Genes Preferentially Expressed In Anthersmentioning

confidence: 99%

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Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

Kang

Zhang

Bonnema³

et al. 2007

Plant Mol Biol

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The dominant male sterility gene Ms-cd1 is identified in Brassica oleracea. Electron microscopical observations revealed that abortion of pollen development starts after tetrad formation. This important male sterility phenotype is characterized by lack of degradation of the primary pollen mother cell (PMC) wall and delayed degradation of callose surrounding the tetrads and thus arrest of microspore release. Gene expression of the male sterile and fertile buds was analyzed by heterologous hybridization of Brassica oleracea cRNA onto an Arabidopsis whole genome oligonucleotide microarray. A total of 277 suppressed genes including 40 kinase-, 32 cell wall modification and 29 transport related genes were found to be significantly down regulated [3-fold in the male sterile mutant. The vast majority of the differentially expressed transcripts are found to present late pollen stage specific genes. Kinase genes, cell wall modification genes and ion transport genes were greatly over-represented when compared to their percentage of all flower bud expressed genes and represent 36.5% of the genes suppressed by Ms-cd1. Our results also suggest that Ms-cd1 may blocks an anther developmental pathway with a small number of genes suppressed in tapetum cells which prevent the degradation of callose and PMC wall, which further leads to the suppression of a large number of genes involved in signaling pathways, cell wall modification and ion transport in pollen grains.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Genes Preferentially Expressed In Anthersmentioning

confidence: 99%

Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

Kang

Zhang

Bonnema³

et al. 2007

Plant Mol Biol

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“…In Arabidopsis, the generative cell undergoes another mitotic division giving rise to two sperm cells (i.e., the tricellular pollen stage). Following pollination, the vegetative cell controls the further development of the mature pollen grain and growth of the pollen tube into the style until both sperm cell nuclei are delivered to the embryo sac in the ovule, where they participate in double fertilization [1,2].The last two decades have been marked by increasing efforts to decipher the genetic and molecular basis of pollen development and functions [reviewed in 1,3,4]. In the model plant Arabidopsis thaliana, the extremely reduced, tricellular male gametophyte constitutes an ideal experimental system for analyses of important biological processes in higher plant reproduction.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…The last two decades have been marked by increasing efforts to decipher the genetic and molecular basis of pollen development and functions [reviewed in 1,3,4]. In the model plant Arabidopsis thaliana, the extremely reduced, tricellular male gametophyte constitutes an ideal experimental system for analyses of important biological processes in higher plant reproduction.…”

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confidence: 99%

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A reference map of the Arabidopsis thaliana mature pollen proteome

Noir

Bräutigam

Colby

et al. 2005

Biochemical and Biophysical Research Communications

134

145

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The male gametophyte (or pollen) plays an obligatory role during sexual reproduction of higher plants. The extremely reduced complexity of this organ renders pollen a valuable experimental system for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth. Here, we present the first reference map of the mature pollen proteome of the dicotyledonous model plant species, Arabidopsis thaliana. Based on two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight, and electrospray quadrupole time-of-flight mass spectrometry, we reproducibly identified 121 different proteins in 145 individual spots. The presence, subcellular localization, and functional classification of the identified proteins are discussed in relation to the pollen transcriptome and the full protein complement encoded by the nuclear Arabidopsis genome. Ó 2005 Elsevier Inc. All rights reserved.Keywords: Arabidopsis thaliana; Male gametophyte; Mass spectrometry; Mature pollen; Proteome; Two-dimensional gel electrophoresis In higher plants, development of the male gametophyte is a well-programmed and elaborate process. Male sporogenesis begins with the division of a diploid sporophytic cell giving rise to the anther wall of the stamen and the sporogenic cells. The latter cells then undergo several mitoses to differentiate into pollen mother cells. Subsequently, each diploid pollen mother cell forms a tetrad of haploid microspores via a round of DNA replication followed by two consecutive meiotic divisions. Each uninucleate microspore then undergoes an asymmetric mitotic division forming a large vegetative cell and a smaller generative cell (i.e., the bicellular pollen stage). In Arabidopsis, the generative cell undergoes another mitotic division giving rise to two sperm cells (i.e., the tricellular pollen stage). Following pollination, the vegetative cell controls the further development of the mature pollen grain and growth of the pollen tube into the style until both sperm cell nuclei are delivered to the embryo sac in the ovule, where they participate in double fertilization [1,2].The last two decades have been marked by increasing efforts to decipher the genetic and molecular basis of pollen development and functions [reviewed in 1,3,4]. In the model plant Arabidopsis thaliana, the extremely reduced, tricellular male gametophyte constitutes an ideal experimental system for analyses of important biological processes in higher plant reproduction. In addition, it represents a very useful model for studying fundamental aspects of plant biology such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth [5][6][7].The availability of the full genome sequence of Arabidopsis [8] has made genome-wide, microarray-based analyses of the male gametophyte transcriptome of this model plant possible [9][10][11][12]. 13,977 male gametophyte-expressed mRNAs were recently identified using the ATH1 Genome Array, which cover...

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Male Sterility and Hybrid Production Systems

Oliver

2004

Handbook of Plant Biotechnology

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The ability to exploit the long known phenomenon of hybrid vigor or heterosis, the superior performance of the F1 hybrid compared to either parent line, has greatly impacted the goal of production agriculture to meet world food demands. Hybrid production systems rely on an efficient and effective mechanism for inducing male sterility in one of the parental lines in order to ensure purity of the resultant hybrid seed. Natural male sterility systems are available but are limited in their availability for all crops and their effectiveness in those for which they have been developed. This chapter discusses the means by which modern techniques of genetic engineering have broadened our ability to develop male sterile lines and improve hybrid production strategies. The chapter discusses the development of dominant male sterility systems using genetic ablation and developmental disruption strategies, conditional male sterility strategies and the genetic engineering of maintainer lines. The generation of novel and improved systems for hybrid production in the ongoing search for crop improvement has been, and continues to be, one of the major targets for transgenic manipulations within the agricultural biotechnological community.

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Male Gametophyte Development

Cited by 327 publications

References 0 publications

Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray

A reference map of the Arabidopsis thaliana mature pollen proteome

Male Sterility and Hybrid Production Systems

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