Male chromosomes of sea urchin hybrid andromerogones created with cryopreserved sperm

Saotome, Kyoko; Kamimura, Ryuichi; Kurokura, Hisashi; Hirano, Reijiro

doi:10.2108/zsj.19.185

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…In addition, sex-specific markers have not been identified, and the sexdetermination mechanism of sea urchin remains undetermined. According to previous reports, the chromosomes of sea urchins are too small to be analyzed accurately, and heteromorphic sex chromosomes cannot be observed in most species (Saotome, 1987;Saotome et al, 2002;Eno et al, 2009). As an exception, a heteromorphic chromosome has been observed in Paracentrotus lividus sea urchins, for which an XX/XY sex-determination system has also been deduced (Lipani et al, 1996).…”

Section: Discussionmentioning

confidence: 97%

Identification of Sex-Specific Markers Through 2b-RAD Sequencing in the Sea Urchin (Mesocentrotus nudus)

et al. 2021

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Sex-specific markers play an important role in revealing sex-determination mechanism. Sea urchin (Mesocentrotus nudus) is an economically important mariculture species in several Asian countries and its gonads are the sole edible parts for people. However, growth rate and immunocompetence differ by sex in this species, sex-specific markers have not been identified, and the sex-determination mechanism of sea urchin remains undetermined. In this study, type IIB endonuclease restriction-site associated DNA sequencing (2b-RAD-seq) and a genome survey of M. nudus were performed, and three female-specific markers and three female heterogametic single nucleotide polymorphism (SNP) loci were identified. We validated these sex-specific markers via PCR amplification in a large number of individuals, including wild and artificially bred populations. Several open reading frames (ORFs) were predicted, although there are no potential genes known for sex determination and sex differentiation within the scaffold in which the sex-specific markers are located. Importantly, the female-specific sequences and female heterozygous SNP loci indicate that a female heterogametic and male homogametic ZW/ZZ sex-determination system should exist in M. nudus. The results provide a solid basis for revealing the sex-determination mechanism of this species, and open up new possibilities for developing sex-control breeding in sea urchin.

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Section: Discussionmentioning

confidence: 97%

Identification of Sex-Specific Markers Through 2b-RAD Sequencing in the Sea Urchin (Mesocentrotus nudus)

et al. 2021

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“…The resulting embryos were passed through a Nytex filter (120 m) to physically remove the fertilization membrane and to dissociate the blastomeres. The resulting blastomeres were treated in 8% sodium citrate solution and washed three times with Carnoy's fixative (Saotome et al, 2002); then 10-l aliquots were placed on heated (46°C), positively charged slides (Fisher Scientific, Colorfrost Plus slides). Blastomeres in each aliquot were spread (squashed) under coverslips manually using thumb pressure on a coverslip (22 ϫ 22 mm).…”

Section: Blastomere Dissociation and Chromosomal Spreadsmentioning

confidence: 99%

Methods for Karyotyping and for Localization of Developmentally Relevant Genes on the Chromosomes of the Purple Sea Urchin, Strongylocentrotus purpuratus

Eno

Böttger

Walker

2009

The Biological Bulletin

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The purple sea urchin, Strongylocentrotus purpuratus, is the only non-chordate deuterostome model with a fully sequenced genome. Chromosomal localization of individual genes and resulting gene maps are unavailable for this or for any sea urchin. As a result, the purple sea urchin genome has not been mapped onto specific chromosomes and remains inaccessible to genome-wide approaches addressing questions that require positional information for particular genes. Here we describe the first successful methods for karyotyping and localizing specific gene loci on chromosomes of Strongylocentrotus purpuratus and those of the phylogenetically related Strongylocentrotus droebachiensis. Both species have 42 chromosomes in their diploid genomes (n = 21). There are 2 large, 8 medium, and 10 small pairs, plus one putative sex pair. In both species, bindin genes were localized to 2 pair of homologous chromosomes by fluorescent in situ hybridization. Fluorescently labeled bacterial artificial chromosome clones generated from S. purpuratus for the functionally related genes brachyury, foxa, and foxb were localized to different chromosomes. Our protocols provide previously unavailable tools for developing a gene map for the purple sea urchin genome.

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Evolution of neuronal signalling: Transmitters and receptors

Hoyle¹

2011

Autonomic Neuroscience

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Male chromosomes of sea urchin hybrid andromerogones created with cryopreserved sperm

Cited by 3 publications

References 15 publications

Identification of Sex-Specific Markers Through 2b-RAD Sequencing in the Sea Urchin (Mesocentrotus nudus)

Identification of Sex-Specific Markers Through 2b-RAD Sequencing in the Sea Urchin (Mesocentrotus nudus)

Methods for Karyotyping and for Localization of Developmentally Relevant Genes on the Chromosomes of the Purple Sea Urchin, Strongylocentrotus purpuratus

Evolution of neuronal signalling: Transmitters and receptors

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