Male biased gene flow in banana pseudostem weevil (Odoiporus longicollis Oliver) as revealed by analysis of the COI-tRNALeu COII region

Abstract

The genetic diversity amongst thirty weevils representing six Indian populations of banana pseudostem weevil, i.e., Odoiporus longicollis (Oliver) was estimated by sequence analysis of the partial COI-tRNA(Leu)-COII region. The sequences exhibited AT bias typical of insect mitochondrial DNA which was highest in the first codon position of COI and in the third codon position of COII. There was no phylogeographic distribution of the populations. The Fu and Li's D and F tests were non-significant for this mitochondrial region. No Wolbachia infection was detected in any of the populations. The genetic differentiation amongst the populations was highly significant (p < 0.001; χ2 = 123.333; df = 75), suggesting restricted gene flow between the populations. This result did not correlate with that obtained with nuclear rDNA markers, i.e., ITS1 and ITS2, suggesting a male biased gene flow between the populations.

DIVERSITY OF Wasmannia auropunctata (HYMENOPTERA: FORMICIDAE) AND THE USE OF MITOCHONDRIAL INTERGENIC SPACER AND LEUCINE tRNA FOR ITS IDENTIFICATION

“…, Souza et al (2009 used randomly ampli ed polymorphic DNA (RAPD) to distinguish between populations of W. auropunctata and Wasmannia rochai (Forel, 1912) and determine their genetic variability. e mtDNA COI-COII region has also been used as the main or auxiliary tool in evolutionary studies of several genera of ants (Feldhaar et al, 2003;Janda et al, 2004;Shoemaker et al, 2006;Bacci Jr et al, 2009) and numerous other insects (Blum et al, 2003;Memon et al, 2006;Nakahara and Muraji, 2008;Tatarnic et al, 2013;Shankar et al, 2015;Zanini et al, 2015). According to Hwang and Kim (1999), the use of protein-coding genes at the species or population level is justi ed by their high rate of substitution in the third codon position.…”