Male-biased Adult Production of the Striped Fruit Fly, Zeugodacus scutellata, by Feeding dsRNA Specific to Transformer-2

Baki, Abdullah Al; Vatanparast, Mohammad; Kim, Yonggyun

doi:10.3390/insects11040211

Cited by 7 publications

(7 citation statements)

References 43 publications

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“…Using chitin synthase gene A (SeCHSA) as the target gene, the growth and development of Spodoptera exigua larvae fed by E. coli containing dsRNA of SeCHSA was disturbed, and then the mortality rates in the 5th instar larvae increased signi cantly [43]. After either feeding or injecting dsRNA of the sex-determining gene Transformer-2 to Zeugodacus scutellata, the Transformer-2 gene were all silenced, and increased signi cantly the number of male among their offspring [44]. Through RNAi to the appendage terminal Distal-less (Dll) gene of D. magna, it was found that the appendage terminal was de cient [45].…”

Section: Discussionmentioning

confidence: 99%

Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis

Cao

Zhao

et al. 2022

Preprint

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Background Molting is an important physiological process in the growth and development of arthropoda, which is mainly regulated by juvenile hormone and ecdysone. CYP302A1 is a key enzyme which plays a critical role in the synthesis of ecdysone in insects, but it has not been identified in cladocera. Results The CYP302Al gene of D. sinensis was cloned and its function was analyzed in this paper. The CYP302Al gene of D. sinensis was 5926 bp in full-length, with an open reading frame (ORF) of 1596 bp that encoded 531 amino acids, a molecular weight of 60.82 kDa and an isoelectric point of 9.29. The amino acid sequence analysis revealed that there were five characteristic conserved regions of cytochrome P450 family (namely helix-C, helix-K, helix-I, PERF and heme-binding). In dsRNA mediated experiment, the expression level of CYP302A1 gene decreased significantly (knock-down of 56.22%) in the 5% Escherichia coli concentration treatment. In addition, the expression levels of EcR and USP and HR3 genes in the downstream decreased also significantly, whereas that of FTZ-f1 gene increased significantly. In the 5% E. coli concentration treatment, the molting time at first pregnancy of D. sinensis prolonged, and the development of embryos in the incubation capsule appeared abnormal or disintegrated. The whole-mount in situ hybridization showed that the CYP302A1 gene of D. sinensis had six expression sites before RNA interference (RNAi), which located in the first antennal ganglion, ovary, gastric coeca, olfactory hair, thoracic limb and tail spine. However, the expression signal of the CYP302A1 gene of D. sinensis disappeared in the first antennal ganglion and obviously attenuated in the ovary after RNAi. Conclusions In conclusion, the CYP302A1 gene played an important role in the ecdysone synthesis pathway of D. sinensis, and the knock-down of the gene affected the molting and reproduction of D. sinensis.

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Section: Discussionmentioning

confidence: 99%

Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis

Cao

Zhao

et al. 2022

Preprint

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“…RNAi is useful for studying gene function in insects. Microinjection and artificial diets are the primary methods used to deliver dsRNA 39–44 . For D. citri and other small insects, feeding on an artificial diet with added dsRNA is the most effective strategy 30,45,46 .…”

Section: Discussionmentioning

confidence: 99%

NADPH‐cytochrome P450 reductase mediates the susceptibility of Asian citrus psyllid Diaphorina citri to imidacloprid and thiamethoxam

Yuan

Jing

et al. 2020

Pest Management Science

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BACKGROUND The Asian citrus psyllid Diaphorina citri has developed high levels of resistance to many insecticides, and understanding its resistance mechanism will aid in the chemical control of this species. Nicotinamide adenine dinucleotide phosphate (NADPH)‐cytochrome P450 reductase (CPR) is crucial in cytochrome P450 function, and in some insects CPR knockdown has increased their susceptibility to insecticides. However, the CPR from D. citri has not been characterized and its function is undescribed. RESULTS The CPR gene of D. citri (DcCPR) was cloned and sequenced. The expression level of DcCPR, determined by reverse‐transcription quantitative polymerase chain reaction (RT‐qPCR) analysis, was highest in the midgut and in nymphs. After feeding on double‐stranded RNA for 72 h, the DcCPR messenger RNA level in D. citri adults decreased by 68.4%, and the susceptibility of D. citri to imidacloprid and thiamethoxam significantly increased. Meanwhile, after DcCPR silencing, the specific activities of DcCPR protein and P450s were significantly reduced by 41.6% and 44.7%, respectively. The subsequent western blot analysis and quantification of band intensity also showed that DcCPR content significantly decreased, consistent with the results of the specific activity test. In a eukaryotic expression assay, the viability of cells expressing DcCPR was significantly higher than the viability of cells expressing green fluorescent protein (GFP) when cells were exposed to imidacloprid or thiamethoxam. CONCLUSION These results indicate that DcCPR contributes to D. citri susceptibility to imidacloprid and thiamethoxam.

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“…Results were plotted using Sigma plot 10.0. Means were compared by least squared difference (LSD) test of one-way analysis of variance (ANOVA) using PROC GLM of SAS program 75 , 76 and discriminated at Type I error = 0.05.…”

Section: Methodsmentioning

confidence: 99%

Cold tolerance strategies of the fall armyworm, Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Vatanparast

Park

2022

Sci Rep

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The fall armyworm (FAW), Spodoptera frugiperda, is native to the tropical and subtropical areas of the American continent and is one of the world's most destructive insect pests and invaded Africa and spread to most of Asia in two years. Glycerol is generally used as a cryoprotectant for overwintering insects in cold areas. In many studies, the increase in glycerol as a main rapid cold hardening (RCH) factor and enhancing the supercooling point was revealed at low temperatures. There are two genes, including glycerol-3-phosphate dehydrogenase (GPDH) and glycerol kinase (GK), that were identified as being associated with the glycerol synthesis pathway. In this study, one GPDH and two GK sequences (GK1 and GK2) were extracted from FAW transcriptome analysis. RNA interference (RNAi) specific to GPDH or GK1 and GK2 exhibited a significant down-regulation at the mRNA level as well as a reduction in survival rate when the RNAi-treated of FAW larvae post a RCH treatment. Following a cold period, an increase in glycerol accumulation was detected utilizing high-pressure liquid chromatography and colorimetric analysis of glycerol quantity in RCH treated hemolymph of FAW larvae. This research suggests that GPDH and GK isozymes are linked to the production of a high quantity of glycerol as an RCH factor, and glycerol as main cryoprotectant plays an important role in survival throughout the cold period in this quarantine pest studied.

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Male-biased Adult Production of the Striped Fruit Fly, Zeugodacus scutellata, by Feeding dsRNA Specific to Transformer-2

Cited by 7 publications

References 43 publications

Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis

Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis

NADPH‐cytochrome P450 reductase mediates the susceptibility of Asian citrus psyllid Diaphorina citri to imidacloprid and thiamethoxam

Cold tolerance strategies of the fall armyworm, Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

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