Male antifertility compounds from Tripterygium wilfordii Hook F.

Matlin, Stephen A.; Belenguer, Ana M.; Stacey, Vivlen E.; Qlan, Shao Zhen; Yang, Xu; Zhang, Jlan Wel; Sanders, Jeremy K. M.; Amor, Stuart R.; Pearce, Clive M.

doi:10.1016/0010-7824(93)90036-7

Cited by 82 publications

(35 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…f and one of its main active components, triptolide, a diterpenoid triepoxide, have been shown to have anti-inflammatory, 27,28) contraceptive 29,30) and anti-neoplastic 8,31) activities. However, the clinical uses of triptolide were limited because of its severe toxicities.…”

Section: Discussionmentioning

confidence: 99%

Involvement of Mitochondrial Pathway in Triptolide-Induced Cytotoxicity in Human Normal Liver L-02 Cells

Yao

Jiang

Duan³

et al. 2008

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Involvement of Mitochondrial Pathway in Triptolide-Induced Cytotoxicity in Human Normal Liver L-02 Cells

Yao

Jiang

Duan³

et al. 2008

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

“…Compounds 30-32 significantly reduced sperm counts and sperm motility in rats (Matlin et al, 1993;Zhang et al, 1993;. Several microscopic studies on rats fed 36 showed deformed sperm and possibly also negative effects on the epididymis (Ye et al, 1991(Ye et al, , 1994Feng et al, 1993;Dang et al, 1995;.…”

Section: Antifertilitymentioning

confidence: 99%

“…Tripchlorolide (36) is also known as T 4 , triptonide (30) as T 7 , tripdiolide (31) as T 8 , triptolidenol (32) as T 9 , triptolide (1) as T 10 , triptriolide (34) as T 11 , and 16-hydroxytriptolide (33) as L 2 Li, 1993). Tripchlorolide (36) may be an artifact of isolation and can spontaneously reconvert to 1 (Matlin et al, 1993), which suggests that the biological activity reported for this compound may actually be due to 1. 5.2.1.1.…”

Section: 21mentioning

confidence: 99%

Medicinal chemistry and pharmacology of genus Tripterygium (Celastraceae)

et al. 2007

View full text Add to dashboard Cite

Plants in the genus Tripterygium, such as Tripterygium wilfordii Hook. f., have a long history of use in traditional Chinese medicine. In recent years there has been considerable interest in the use of Tripterygium extracts and of the main bioactive constituent, the diterpene triepoxide triptolide (1), to treat a variety of autoimmune and inflammation-related conditions. The main mode of action of the Tripterygium extracts and triptolide (1) is the inhibition of expression of proinflammatory genes such as those for interleukin-2 (IL-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2) and interferon-gamma (IFN-γ). The efficacy and safety of certain types of Tripterygium extracts were confirmed in human clinical trials in the US and abroad. Over 300 compounds have been identified in the genus Tripterygium, and many of these have been evaluated for biological activity. The overall activity of the extract is based on the interaction between its components. Therefore, the safety and efficacy of the extract cannot be fully mimicked by any individual constituent. This review discusses the biochemical composition and biological and pharmacological activities of Tripterygium extracts, and their main bioactive components.

show abstract

“…Numerous studies have shown that triptolide has antiinflammatory effects (22,23), contraceptive activity (24,25), and anti-neoplastic abilities (26,27). However, due to it severe toxic effects the clinical use of triptolide is limited.…”

Section: Discussionmentioning

confidence: 99%

Triptolide induces apoptosis in human adrenal cancer NCI-H295 cells through a mitochondrial-dependent pathway

Yang

2011

Oncol Rep

View full text Add to dashboard Cite

Abstract. Triptolide, the main active component obtained from Tripterygium wilfordii Hook. f, has been reported to present potent immunosuppressive and anti-inflammatory biological activities. It has been previously shown that due to the cytotoxicity of triptolide it has a limited use in the clinic. Although numerous studies have shown that triptolide induced apoptosis in many human cancer cells there is no report to show triptolide-induced apoptosis in human adrenal cancer cells. We treated the human adrenal cancer NCI-H295 cells with triptolide in vitro and investigated its cytotoxic effects. The cytotoxicity was examined and quantitated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and the viability of inhibition and apoptosis was determined by flow cytometric assay, using propidium iodide (PI) staining for apoptosis. Flow cytometric assay also used for the determination of reactive oxygen species (ROS) production and the levels of mitochondrial membrane potential (ΔΨ m ), and the caspase-3 and -9 activation in NCI-H295 cells. Western blotting was used for examining the changes of apoptotic associated proteins. The results indicated that triptolide induced cytotoxicity (decreased the percentage of viable cells) and induced sub-G1 phase (apoptosis) occurring in NCI-H295 cells and those effects are dose-dependent. Results also showed that triptolide promoted the production of ROS and decreased the levels of ΔΨ m in examined NCI-H295 cells. The results showed that triptolide promoted the levels of cytochrome c, Apaf-1, AIF, Endo G, caspase-9 and -3 which were analyzed by Western blotting. These results suggest that triptolide is able to induce apoptosis on NCI-H295 cells through the mitochondrial-dependent signal pathway.

show abstract

Male antifertility compounds from Tripterygium wilfordii Hook F.

Cited by 82 publications

References 7 publications

Involvement of Mitochondrial Pathway in Triptolide-Induced Cytotoxicity in Human Normal Liver L-02 Cells

Involvement of Mitochondrial Pathway in Triptolide-Induced Cytotoxicity in Human Normal Liver L-02 Cells

Medicinal chemistry and pharmacology of genus Tripterygium (Celastraceae)

Triptolide induces apoptosis in human adrenal cancer NCI-H295 cells through a mitochondrial-dependent pathway

Contact Info

Product

Resources

About