Male and Female Effects on the <i>In Vitro</i> Production of Bovine Embryos

Palma, G.A.; Sinowatz, Fred

doi:10.1111/j.1439-0264.2004.00543.x

Cited by 56 publications

(51 citation statements)

References 28 publications

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“…Previous investigations have shown that, at least, a part of these variations may be because of different capacities of bulls to produce embryos and a high male effect on the efficiency of an IVF program [49]. Fertilization rates were 10% to 20% lower with regard to IVP using nonsorted sperm [3].…”

Section: Discussionmentioning

confidence: 90%

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

et al. 2012

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In this work we analyzed the effects of three culture systems on developmental ability of bovine embryos in vitro produced with sexed sperm, the survival to vitrification (cryologic vitrification method) of such blastocysts, and their pregnancy rates after embryo transfer to recipients, both as fresh and after vitrification/warming. Finally, we measured the accuracy of the sorting protocol by a polymerase chain reaction-based method to validate the embryo sex at blastocyst stages. We confirmed an individual effect of the bull as well as development rates of embryos produced with sorted sperm lower than embryos with unsorted sperm, independent of the culture system used. The cryoresistance to vitrification of embryos produced with sexed sperm did not differ from that of conventionally produced embryos (re-expansion rates at 24 and 48 h: 74.6% vs. 75.5%, and 64.5% vs. 68.1% for embryos produced with conventional and sorted sperm, respectively; hatching rates at 48 h: 63.55% vs. 55.5% for embryos produced with conventional and sorted sperm, respectively). Finally, no significant differences were found in pregnancy rates after the embryo transfer of fresh and vitrified/warmed blastocysts (52.8% vs. 42.0%, respectively; P Ͼ 0.05). Male and female embryos produced with sorted sperm showed the same quality in terms of developmental ability, cryoresistance, and pregnancy rates after transfer. Our culture system, coupled with the vitrification in fiber plugs, provides good quality sex-known embryos which survive vitrification at similar rates than embryos produced with conventional unsorted sperm; also it produces good pregnancy rates after transfer of sexed embryos both fresh and after vitrification and warming.

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Section: Discussionmentioning

confidence: 90%

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

et al. 2012

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“…However, a high variability in the outcome of bovine embryo IVP has been reported (Palma & Sinowatz 2004, Alomar et al 2008. One of the reasons proposed to explain such variation is the impaired integrity of sperm chromatin (Boe-Hansen et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Simões¹,

Feitosa²,

Siqueira³

et al. 2013

Reproduction

118

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Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%).In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.

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“…It has been shown that in cattle, as in other farm animals, the male effect on IVP leads to high variability in fertilization rates and the effi ciency of embryo development (Chamberland et al, 2001;Vassena et al, 2003;Palma and Sinowatz, 2004;Kątska-Książkiewicz et al, 1996, 2006. The question arises whether it would be possible to overcome the bull effect and increase effi ciency of blastocysts production by using selected, competent oocytes for IVF.…”

Section: Introductionmentioning

confidence: 99%

Effects of oocyte quality and semen donor on the efficiency of <i>in vitro</i> embryo production in cattle

Kątska-Książkiewicz¹,

Opiela²,

Ryńska³

2009

J. Anim. Feed Sci.

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The aim of the study was to determine whether immature bovine oocytes selected by morphology and BCB test (brilliant cresyl blue) and used for in vitro embryo production would increase the blastocyst rate when inseminated with sperm derived from bulls of reduced in vitro fertilizability.To relate oocyte quality and bull effect to blastocyst quality, morphology, ability for hatching and caspase-3 activity (marker of apoptosis) were evaluated.Both, oocytes originating from BCB+ and from Control COCs exhibited higher cleavage and blastocyst rates (P<0.001) compared with BCB-oocytes. Signifi cant differences in cleavage and blastocyst rates between the two bulls were observed. The highest blastocyst rate was noticed for BCB+ oocytes, regardless the bull. Comparison of the caspase-3 activity shows a signifi cant overbalance of blastocysts with high caspase-3 activity, regardless the stage of blastocyst development and their origin. Thus, using excellent quality oocytes and sperm with reduced in vitro fertilizability it was possible to some extent to overcome bull effect on embryo production.

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Male and Female Effects on the In Vitro Production of Bovine Embryos

Cited by 56 publications

References 28 publications

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

In vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Effects of oocyte quality and semen donor on the efficiency of <i>in vitro</i> embryo production in cattle

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