Male advantage observed for in vitro fertilization mouse embryos exhibiting early cleavage

Kawase, Yoshiaki; Tachibe, Takanori; Kamada, Nobuo; Jishage, Kou-ichi; Watanabe, Hiroyuki; Suzuki, Hiroshi

doi:10.1002/rmb2.12355

Cited by 6 publications

(5 citation statements)

References 16 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding “poor-quality embryos”, developed/cleavaged embryos were selected (at 2–4-6 cells) at 53 h, with intact and good morphology of the cells. These “poor-quality” embryos could have stopped the development, or have an altered development or be only “very slow”, since part of them reached the blastocyst stage (blastocyst rate 11%), in line with previous studies [ 42 – 45 ]. Since more likely, there was a mix of blocked, altered but also simply delayed embryos, we did not call them “bad quality embryos” or embryos with impaired development.…”

Section: Discussionsupporting

confidence: 85%

“…The timing to assign embryos to good- or poor-quality embryo groups was based on the objectives of the study, the experimental conditions, and previous studies [ 42 – 45 ]. According to these studies, selecting embryos at 53 h postinsemination allows to have a pool of embryos with fast, moderate, and slow development in the “good-quality embryos” group.…”

Section: Discussionmentioning

confidence: 99%

“…Embryos which at 53 h were not developed (not cleaved) or were degenerated or fragmented were not used. The classification of the embryos in good- and poor-quality was based on previous studies, selecting a pool of fast, moderate and slow embryos in the “good embryos”, since selecting fast embryos only could bias the selection towards more male embryos [ 42 – 45 ]. Then, both G and P embryos were co-cultured in groups of 25 embryos with frozen-thawed BOEC monolayers (~ 85% of confluence) or alone for 24 h, as follows: 1) BGE: BOEC co-cultured with G embryos; BPE: BOEC co-cultured with P embryos; GE: culture of G embryos without cells; PE: culture of P embryos without cells; and as controls, B: BOEC alone as well as M: medium alone.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

Hamdi,

Sánchez,

Fernandez-Fuertes

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background Increasing evidence points to an active role of oviductal extracellular vesicles (oEVs) in the early embryo-maternal dialogue. However, it remains unclear whether oEVs contribute to the recognition of the presence of embryos and their quality in the oviduct. Hence, we examined whether the molecular cargo of oEVs secreted by bovine oviduct epithelial cells (BOEC) differs depending on the presence of good (≥ 8 cells, G) or poor (< 8 cells, P) quality embryos. In addition, differences in RNA profiles between G and P embryos were analyzed in attempt to distinguish oEVs and embryonic EVs cargos. Methods For this purpose, primary BOEC were co-cultured with in vitro produced embryos (IVP) 53 h post fertilization as follows: BOEC with G embryos (BGE); BOEC with P embryos (BPE); G embryos alone (GE); P embryos alone (PE); BOEC alone (B) and medium control (M). After 24 h of co-culture, conditioned media were collected from all groups and EVs were isolated and characterized. MicroRNA profiling of EVs and embryos was performed by small RNA-sequencing. Results In EVs, 84 miRNAs were identified, with 8 differentially abundant (DA) miRNAs for BGE vs. B and 4 for BPE vs. B (P-value < 0.01). In embryos, 187 miRNAs were identified, with 12 DA miRNAs for BGE vs. BPE, 3 for G vs. P, 8 for BGE vs. GE, and 11 for BPE vs. PE (P-value < 0.01). Conclusions These results indicated that oEVs are involved in the oviductal-embryo recognition and pointed to specific miRNAs with signaling and supporting roles during early embryo development.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

Hamdi,

Sánchez,

Fernandez-Fuertes

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…However, several studies have suggested that blastocyst transfer can increase the proportion of male embryos (3,36,37). Because male embryos develop faster than female embryos and have better morphology, the faster-growing embryos are more likely to be male (38). Additionally, studies have shown that compared with female embryos developed during the same period, the morphological score of male embryos was better; for example, 72% of D5 or D6 blastocysts were male embryos, whereas 60% of D3 blastocysts were female embryos (39).…”

Section: Discussionmentioning

confidence: 99%

Ideal Stage of Embryo Transfer to Improve Outcomes for Women of Advanced Maternal Age

Xiao¹,

Sun²,

Zhen³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background The purpose of this retrospective study was to optimise the transplantation strategy for women of advanced maternal age to achieve live births within the shortest time. Methods Data were collected from patients older than 40 years who underwent assisted reproductive therapy at our centre from 1 January 2009 to 31 December 2019. A total of 1233 cases of fresh cleavage embryo transfer cycles, 280 cases of frozen-thawed blastocyst transfer cycles, and 26 cases of frozen-thawed cleavage embryo transfer cycles were included. Multivariable logistic regression was performed to adjust for confounding factors. Results The main outcome was the live birth rate. The secondary outcomes were the clinical pregnancy rate, spontaneous abortion rate, and neonatal outcomes. We found that the blastocyst formation rate of patients older than 40 years was 23.5%, the freezing cycle rate was 19.8%, and the fresh embryo transfer rate was 83.0%. Conclusions Cleavage embryo transfer should be performed first to reduce the cycle cancellation rate. If the number of retrieved oocytes is more than eight, then blastocyst transplantation can be considered after fully discussing the advantages and disadvantages of blastocyst culture with patients. Alternatively, cleavage embryo transfer can be performed first, and frozen-thawed blastocyst transfer can be performed next if cleavage embryo transfer is unsuccessful.

show abstract

“…IVF was performed as previously described [ 24 ]. Young female ICR mice (3–6 weeks old) and adult male ICR mice (14–20 weeks old) were used for fertilization.…”

Section: Methodsmentioning

confidence: 99%

LPS Administration during Fertilization Affects Epigenetic Inheritance during Embryonic Development

Kim

Yoneda

Tomita

et al. 2023

Animals

Self Cite

View full text Add to dashboard Cite

Intrauterine inflammation can cause infertility by disrupting reproductive function. The pathogenesis underlying this process may primarily involve endotoxins from lipopolysaccharides (LPS), which are produced by Gram-negative bacteria. However, the long-term effects of endotoxins in mammalian pregnancy following LPS exposure during fertilization have not been clarified. In this study, we performed experiments to analyze the influence of LPS on early embryonic development and fetal development in mice. Mice uteruses were examined for the expression of genes related to the inflammatory response. The expression of Il-1β and Il-6 increased following the administration of 200 and 1000 µg/kg LPS. Exposure to LPS using in vitro fertilization (IVF) significantly decreased the embryonic developmental rate. A concentration of 100 µg/kg LPS significantly increased the placental weight and fetal crown –rump length (CRL), whereas a concentration of 200 µg/kg LPS significantly decreased the placenta weight and fetal weight in vivo. These findings indicate that maternal LPS during fertilization affects fetal development until the late stage of pregnancy. Thus, maternal endotoxins may affect epigenetic inheritance during embryonic development from the early to late stages of pregnancy.

show abstract

Male advantage observed for in vitro fertilization mouse embryos exhibiting early cleavage

Cited by 6 publications

References 16 publications

Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

Ideal Stage of Embryo Transfer to Improve Outcomes for Women of Advanced Maternal Age

LPS Administration during Fertilization Affects Epigenetic Inheritance during Embryonic Development

Contact Info

Product

Resources

About