MALDI-TOF MS in the Microbiology Laboratory: Current Trends

Schubert, Sören; Kostrzewa, Markus

doi:10.21775/cimb.023.017

Cited by 99 publications

(78 citation statements)

References 9 publications

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“…Identification and diagnosis of affected patients as well as implementation of isolation measures would be greatly facilitated in the case of routine introduction of rapid protein acquisition and analysis, which has a positive impact on patient management and public health (Idelevich et al, 2018;Rodríguez-Sánchez et al, 2019). Preventing the spreading of infection and increasing the curative ratio is very important for the rapid detection, timely treatment and modern disease management of Salmonellosis (Tae-Hoon Kim et al, 2017;Schubert & Kostrzewa, 2017). According to Dieckmann and Malorny (2011), MALDI-TOF MS is the recommended method for clinical practice, based on its reliability, speed and affordability.…”

Section: Discussionmentioning

confidence: 99%

Public Health Impact of Rapid Identification of Epidemiologically Important Characteristics of Salmonella Spp. By Maldi-Tof MS

Novák

Kochlanova

Tvrda

et al. 2019

CBUP

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Introduction: Infections caused by Salmonella are an ongoing worldwide public health problem, often found as a source of nosocomial infections, which cause significant socio-economic burdens. Salmonella is a major food-borne pathogen causing primarily gastrointestinal diseases as well as other localized and systemic infections and extraintestinal complications. MALDI-TOF MS is a new method used by clinical laboratories for rapid, reliable, cost-effective and user-friendly diagnosis of many medical important bacteria of public health interest. The use of this technique improves early identification of genus Salmonella on the species, subspecies and even serovar level, which has a positive impact on public health. Objectives: The aim of the study was to evaluate the importance of MALDI-TOF mass spectrometry for rapid identification of epidemiologically important Salmonella serovars. Based on the latest knowledge about specific biomarker molecules the possibility to identify Salmonella enterica subsp. enterica serovar Enteritidis was verified, which is one of the most common serovars present in Europe associated with gastrointestinal diseases. For serovar Enteritidis a unique mass peak at m/z 6,036 was used. Methods: 140 clinical Salmonella isolates were collected from January to October 2017. Serotyping of Salmonella species, subspecies and serovars was performed by slide agglutination technique: 139 isolates were identified as Salmonella enterica subsp. enterica and one isolate as Salmonella enterica subsp. diarizonae (IIIb). From 139 isolates of Salmonella enterica subsp. enterica the following serovars were detected: 108 Enteritidis, 12 Typhimurium, 6 Infantis, 3 Agona, 3 Derby, 7 others. All isolates were identified also by MALDI-TOF MS as Salmonella spp. For all isolates a unique mass peak at m/z 6.036 was used, which is considered to be relevant for serovar Enteritidis according to the most recent known data. Results: 103 isolates from a total of 108 slide agglutination positive isolates for serovar Enteritidis showed a specific mass signal at m/z 6,036 (+/-). 5 isolates did not contain this specific protein. After repeated analysis from re-culture, the specific protein was found also in the remaining 5 isolates. 32 serovars other than Enteritidis did not contain this specific biomarker molecule. Conclusion: We can confirm that MALDI-TOF MS is a rapid and reliable method to identify of the most common serovar Salmonella Enteritidis based on the diagnostic marker peak at m/z 6,036 identified by recent studies. This unique mass signal showed 100% specificity and 95% sensitivity for Enteritidis serovar in our study. We can conclude that the determination of this frequently present serovar is significantly accelerated by MALDI-TOF MS. The rapid and reliable diagnosis is important for the early treatment and prevention of the spread of salmonellosis with a positive impact on public health.

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Section: Discussionmentioning

confidence: 99%

Public Health Impact of Rapid Identification of Epidemiologically Important Characteristics of Salmonella Spp. By Maldi-Tof MS

Novák

Kochlanova

Tvrda

et al. 2019

CBUP

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“…Ū qPCR -it allows to identify and quantify one bacteria species in one test. Ū MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) -a new reliable tool for identification of bacteria and fungi cultivated on agar plates or in liquid media [3].…”

Section: Microbiome Identificationmentioning

confidence: 99%

The early years of life. Are they influenced by our microbiome?

et al. 2019

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Human microbiome contains the genetic pool of bacteria and other microbes such as Achaea, fungi and viruses inhabiting the human body. It holds an immense potential to affect both physiological and pathological processes. The microbiome's composition can be defined in detail by analyzing ribosomal 16S rRNA and metagenomic tests. Recent increases in cesarean sections, the use of antibiotics during pregnancy, the increasing amount of prematurely born children and changes in infant nutrition have an impact on the microbiome forming process. A correlation between the bowel microbiome's composition and the occurrence of certain diseases, especially inflammatory bowel diseases (IBD), asthma and type 1 diabetes has been demonstrated. The influence on the development of cognitive functions and behaviour has also been displayed. This correlation justifies attempts to restore the beneficial the composition of the microbiome through the use of probiotics, vaginal microflora transfer in case of cesarean section and encouraging breastfeeding. Development of multiple studies on the topic of the human microbiome and its impact on the human body is necessary in order to reach final conclusions. The aim of this article is to summarize recent findings regarding the development of the human microbiome from the first days of life and the influence of changes in its composition on human health.

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“…After incubation at 37 • C for 72 h in an anaerobic jar, strict anaerobes were chosen. Thereafter, after grown on both culture plates, identification of the different colonies was performed by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS), an innovative technology for the rapid and accurate identification of bacterial and fungal isolates in clinical settings [16]. MALDI-TOF technology has been developed to profile, within minutes instead of the usually required 36-48 h of the traditional approaches, based on the pure culture of the microorganism, bacterial proteins from whole cell extracts and to obtain a bacterial fingerprint able to discriminate microorganisms from different genera and species [17].…”

Section: Microbiological Studymentioning

confidence: 99%

Gut Microbiota Imbalance is Related to Sporadic Colorectal Neoplasms. A Pilot Study

et al. 2019

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1) Background: Colorectal cancer (CRC) development is sustained by multiple factors including the gut microbiota, as suggested by a growing body of evidence. Most CRCs have a sporadic (non-hereditary) onset and develop from sporadic colorectal adenomas/polyp (SCA/P). In the present study, we investigated the characteristic of anaerobic microorganisms in stool samples obtained from 20 patients with SCA/P and 20 subjects without evidence of proliferative lesions at colonoscopy (Controls). (2) Material and Methods: We designed this clinical trial using adaptive randomization by minimization. Selective culture media and Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) mass spectrometry techniques were used to identify the components of microbiota. The data obtained revealed a different variability of gut microbiota in stool samples of controls and SCA/P subjects. (3) Results: The most interesting difference was observed for Bacteroides species, which represent the 50% of all bacterial species identified in the stool samples: two species, Bacteroides stercoris and Parabacteroides distasonis, were found only in the feces from control group, whereas Bacteroides fragilis and Prevotella melaningenica species were presents only in SCA/P patients. Among Gram+ bacteria also, specific species were found in the two groups of feces: Clostridium clostridioforme, Propionibacterium avidum and Pediococcus pentasaceus were identified only in controls, while Eubacterium limosum, Clostridium innocuum and Corybebacterium xerosus were identified in SCA/P stool samples only. (4) Conclusions: Our findings suggest that, compared to control stool samples, a different intestinal microbiota is present in SCA/P stool samples, that may create a micro-environment predisposing for the development of proliferative phenomena. As a consequence, gut microbiota manipulation could be a future target for personalized treatments. Author Contributions: Conceptualization, L.P., M.B. and L.S.; Methodology, L.P., M.B. and L.S.; validation, L.P., M.B. and A.M.; investigation, L.P., M.B., A.M. and L.S.; resources, A.D.L.; data curation, M.B., M.T.V., and L.S.; writing-original draft preparation, L.P., M.B. and L.D.; writing-review and editing, L.P., M.B. and L.S.; visualization, M.B. and M.T.; supervision, A.D.L.; project administration, L.P., M.B. and L.S; funding acquisition, M.B. and A.D. Funding: This paper has been partly funded by the Italian Ministry of University and Research-PON-BIOMIS 2018 (Project: Costituzione della Biobanca del Microbiota intestinale e salivare umano: dalla disbiosi alla simbiosi). Conflicts of Interest:The authors declare no conflict of interest.

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MALDI-TOF MS in the Microbiology Laboratory: Current Trends

Cited by 99 publications

References 9 publications

Public Health Impact of Rapid Identification of Epidemiologically Important Characteristics of Salmonella Spp. By Maldi-Tof MS

Public Health Impact of Rapid Identification of Epidemiologically Important Characteristics of Salmonella Spp. By Maldi-Tof MS

The early years of life. Are they influenced by our microbiome?

Gut Microbiota Imbalance is Related to Sporadic Colorectal Neoplasms. A Pilot Study

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