MALDI-TOF MS – application in food microbiology

Akimowicz, Monika; Bucka-Kolendo, Joanna

doi:10.18388/abp.2020_5380

Cited by 20 publications

(21 citation statements)

References 26 publications

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“…In this method, mass of ions is determined by the difference in flight times, where the ions with the same energy levels are directed towards the detector. MALDI-TOF MS provides accurate mass as good as 0.1 [155,164,165].…”

Section: Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometrymentioning

confidence: 99%

Carbon Dots: Classification, Properties, Synthesis, Characterization, and Applications in Health Care—An Updated Review (2018–2021)

2021

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Carbon dots (CDs) are usually smaller than 10 nm in size, and are meticulously formulated and recently introduced nanomaterials, among the other types of carbon-based nanomaterials. They have gained significant attention and an incredible interest in the field of nanotechnology and biomedical science, which is merely due to their considerable and exclusive attributes; including their enhanced electron transferability, photobleaching and photo-blinking effects, high photoluminescent quantum yield, fluorescence property, resistance to photo-decomposition, increased electrocatalytic activity, good aqueous solubility, excellent biocompatibility, long-term chemical stability, cost-effectiveness, negligible toxicity, and acquaintance of large effective surface area-to-volume ratio. CDs can be readily functionalized owing to the abundant functional groups on their surfaces, and they also exhibit remarkable sensing features such as specific, selective, and multiplex detectability. In addition, the physico-chemical characteristics of CDs can be easily tunable based on their intended usage or application. In this comprehensive review article, we mainly discuss the classification of CDs, their ideal properties, their general synthesis approaches, and primary characterization techniques. More importantly, we update the readers about the recent trends of CDs in health care applications (viz., their substantial and prominent role in the area of electrochemical and optical biosensing, bioimaging, drug/gene delivery, as well as in photodynamic/photothermal therapy).

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Section: Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometrymentioning

confidence: 99%

Carbon Dots: Classification, Properties, Synthesis, Characterization, and Applications in Health Care—An Updated Review (2018–2021)

2021

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“…3 ). Today, MALDI-TOF MS is used worldwide as the leading diagnostic instrument for species level identification of medical, pharmaceutical and food-related bacteria [ 45, 46 ] and its use for studying environmental ecosystems is increasing rapidly [ 47 ]. The data produced in the present study demonstrated that MALDI-TOF MS reference libraries, supplemented with reference spectra of the Paraburkholderia species studied, will facilitate species level identification.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Paraburkholderia dioscoreae sp. nov., a novel plant associated growth promotor

Herpell

Vanwijnsberghe

Peeters

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterium, designated strain Msb3T, was recently isolated from leaves of the yam family plant Dioscorea bulbifera (Dioscoreaceae). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that this strain belonged to the genus Paraburkholderia with Paraburkholderia xenovorans as nearest validly named neighbour taxon (99.3 % sequence similarity towards the P. xenovorans type strain). Earlier genome sequence analysis revealed a genome of 8.35 Mb in size with a G+C content of 62.5 mol%, which was distributed over two chromosomes and three plasmids. Here, we confirm that strain Msb3T represents a novel Paraburkholderia species. In silico DNA–DNA hybridization and average nucleotide identity (OrthoANIu) analyses towards P. xenovorans LB400T yielded 58.4 % dDDH and 94.5 % orthoANIu. Phenotypic and metabolic characterization revealed growth at 15 °C on tryptic soy agar, growth in the presence of 1 % NaCl and the lack of assimilation of phenylacetic acid as distinctive features. Together, these data demonstrate that strain Msb3T represents a novel species of the genus Paraburkholderia, for which we propose the name Paraburkholderia dioscoreae sp. nov. The type strain is Msb3T (=LMG 31881T, DSM 111632T, CECT 30342T).

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“…It is a very rapid and easy method in comparison with any other phenotype or genotype identification methods. However, it also has some limitations such as the lack of protein profile of some species in the database and the higher cost of MALDI-TOF MS system [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Nanopore Sequencing (MinION) for the Analysis of Bacteriome and Resistome of Bean Sprouts

2021

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The aspiration these days is to apply rapid methods for parallel analysis of bacteriome and resistome of food samples to increase food safety and prevent antibiotic resistance genes (ARGs) spreading. In this work, we used nanopore sequencing (NS) to determine the diversity and dynamics of the microbiome and resistome in two types of bean sprouts. We proved that NS provided an easy, quick, and reliable way to identify the microbiome and resistome of a food sample also. The species diversity obtained by NS and by cultivation methods with MALDI-TOF MS identification was comparable. In both samples, before and after cultivation (30 °C, 48 h), the dominant part of bacteriome formed Gammaproteobacteria (Enterobacteriaceae, Erwiniaceae, Pseudomonadaceae, Moraxellaceae) and then Firmicutes (Streptococcaceae). The diversity and abundance of single ARGs groups were comparable for both samples despite bacteriome differences. More than 50% of the detected ARGs alignments were mutations conferring resistance to aminoglycosides (16S rRNA), resistance to fluoroquinolones (gyrA, gyrB, parC, parD) and elfamycin (EF-Tu). ARGs encoding efflux pumps formed more than 30% of the detected alignments. Beta-lactamases were represented by many variants, but were less abundant.

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MALDI-TOF MS – application in food microbiology

Cited by 20 publications

References 26 publications

Carbon Dots: Classification, Properties, Synthesis, Characterization, and Applications in Health Care—An Updated Review (2018–2021)

Carbon Dots: Classification, Properties, Synthesis, Characterization, and Applications in Health Care—An Updated Review (2018–2021)

Paraburkholderia dioscoreae sp. nov., a novel plant associated growth promotor

Application of Nanopore Sequencing (MinION) for the Analysis of Bacteriome and Resistome of Bean Sprouts

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