MALDI-TOF mass spectrometry confirms difficulties in separating species of the<i>Avibacterium</i>genus

Alispahic, Merima; Bisgaard, Magne; Heß, Michael; Heß, Claudia

doi:10.1080/03079457.2014.916038

Cited by 20 publications

(15 citation statements)

References 18 publications

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“…Misidentification by MALDI-TOF MS has been associated with insufficient numbers of reference strains within the database (25). The difficulties of confident species identification by MALDI-TOF MS reported in the current case are similar to those reported for another genus of the family Pasteurellaceae, Avibacterium (26). The failure of the MicroSeq system to identify the isolate was essentially due to the absence of M. glucosida from the database.…”

supporting

confidence: 49%

Human Wound Infection with Mannheimia glucosida following Lamb Bite

et al. 2015

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e Mannheimia spp. are veterinary pathogens that can cause mastitis and pneumonia in domestic cattle and sheep. While Mannheimia glucosida can be found as normal flora in oral and respiratory mucosa in sheep, there have been no reported cases of human infection with this organism. CASE REPORTA 64-year-old man sustained a bite on the right thumb from a 10-month-old lamb. The lamb's teeth punctured his thumbnail while he was holding the lamb's mouth open during anthelminthic treatment with a malfunctioning dosing device. He applied epoxy resin to the nail to prevent the shattered edges from catching. Following this application, he developed pain around the wound, and 10 days after the injury, he presented to the hospital. On examination, the nail was discolored, with localized wound tenderness but no discharge, and there was erythema tracking from the right thumbnail up to the axilla. The wound was explored surgically, the nail plate was removed, and pus was washed out and sent to the laboratory for microscopy and culture. The patient was treated with intravenous flucloxacillin for 24 h, followed by oral cephalexin for 7 days. On review 1 week later, the erythema had resolved and the wound had healed well.Microscopy revealed large numbers of polymorphonuclear cells, but no organisms were seen on Gram staining. After 1 day of incubation on horse blood agar, there was heavy growth of a Gram-negative bacillus that was catalase positive, oxidase positive, and indole negative. It was hemolytic on 5% horse blood agar and Mueller-Hinton agar containing 5% sheep blood. After a second day of incubation, a lighter growth of Escherichia coli, Enterococcus faecalis, coagulase-negative staphylococci, and mixed anaerobes was apparent.With the use of the Vitek 2 GN card (bioMérieux, Marcy l'Etoile, France), the Gram-negative bacillus was identified as Sphingomonas paucimobilis (97% match). Notably, the trehalose reaction was negative, the beta-glucosidase reaction was positive, and the ornithine decarboxylase reaction was negative on the Vitek card. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification was performed using the Microflex LT mass spectrometer (Bruker Daltonik), and the results were analyzed using the MALDI Biotyper software program (version 4.0.0.1). The top score for this Gram-negative organism was Mannheimia haemolytica, with a score of 2.177 and the accompanying comment "species of this genus have very similar patterns, therefore distinguishing their species is difficult." Other Mannheimia species appeared in the top 10 identifications listed, including Mannheimia glucosida, the highest score for which was 1.305. Partial 16S rRNA gene sequencing was performed using the MicroSeq 500 bacterial identification kit (Perkin-Elmer/Applied Biosystems, Foster City, CA), with sequence analysis performed on 500 nucleotides using MicroSeq 500 (version 2.2). A result for M. haemolytica was reported, with a specimen score of 43 and a 98.4% match (consensus length of 48...

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confidence: 49%

Human Wound Infection with Mannheimia glucosida following Lamb Bite

et al. 2015

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“…The close‐similarity among MALDI‐TOF profiles of main fish pathogenic species of Aeromonas was reflected by 2D peak distribution diagram (Figure ) showing an overlapping among isolates of different species. This overlapping is likely related with the strong phylogenetic relatedness within the genus Aeromonas (Martin‐Carnahan & Joseph, ) and in line the limited discrimination power of MALDI Biotyper to separate closely related species observed in other microorganisms (Alispahic, Christensen, Bisgaard, Hess & Hess, ; Frey & Kuhnert, ).…”

Section: Resultsmentioning

confidence: 91%

Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

Cerda

Fernández-Bravo

Domı́nguez³

et al. 2018

Journal of Fish Diseases

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The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options, the only species that showed values >90% was A. hydrophila. Overall, when the database was supplemented with 14 new spectra, the number of accurate identifications increased (41% vs. 55%) and the number of inconclusive identifications decreased (45% vs. 29%), but great differences in the success of species-level identifications were found. Species-distinctive mass peaks were identified only for A. hydrophila and A. bestiarum (5003 and 7360 m/z in 95.5% and 94.1% of their isolates, respectively). This work demonstrates the utility of MALDI-TOF MS for Aeromonas identification to the genus level, but there is no consistency for the accurate identification of some of the most prevalent species implicated in fish disease.

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“…MALDI‐TOF‐MS platform has been recently tried on some rare bacteria such as Avibacterium, Myroides, Granulicatella , and Abiotrophia , with comparable results and advantages . In addition, recent reports have shown that both identification and antibiotics susceptibility test results can be obtained together in a few hours from positive blood culture by MALDI‐TOF‐MS .…”

Section: Campylobacter Jejunimentioning

confidence: 99%

Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria

Cheng

Chui

Domish

et al. 2016

Proteomics Clinical Apps

103

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Identification and typing of bacteria occupy a large fraction of time and work in clinical microbiology laboratories. With the certification of some MS platforms in recent years, more applications and tests of MS‐based diagnosis methods for bacteria identification and typing have been created, not only on well‐accepted MALDI‐TOF‐MS‐based fingerprint matches, but also on solving the insufficiencies of MALDI‐TOF‐MS‐based platforms and advancing the technology to areas such as targeted MS identification and typing of bacteria, bacterial toxin identification, antibiotics susceptibility/resistance tests, and MS‐based diagnostic method development on unique bacteria such as Clostridium and Mycobacteria. This review summarizes the recent development in MS platforms and applications in bacteria identification and typing of common pathogenic bacteria.

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MALDI-TOF mass spectrometry confirms difficulties in separating species of theAvibacteriumgenus

Cited by 20 publications

References 18 publications

Human Wound Infection with Mannheimia glucosida following Lamb Bite

Human Wound Infection with Mannheimia glucosida following Lamb Bite

Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria

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