MALDI-TOF mass spectrometry and microsatellite markers to evaluate Candida parapsilosis transmission in neonatal intensive care units

Pulcrano, Giovanna; Roscetto, Emanuela; Iula, Vita Dora; Panellis, Dimitrios; Rossano, Fabio; Catania, Maria Rosaria

doi:10.1007/s10096-012-1642-6

Cited by 65 publications

(48 citation statements)

References 49 publications

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“…Both methods were rapid and effective in highlighting identical strains and studying microevolutionary changes in the yeast population (298). Further investigations are warranted prior to using MALDI-TOF MS for outbreak investigations or rapid antifungal susceptibility testing of yeast isolates; however, these preliminary investigations provide promising results and highlight the diverse utility of the technology.…”

Section: Lichtheimia Sppmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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SUMMARY Within the past decade, clinical microbiology laboratories experienced revolutionary changes in the way in which microorganisms are identified, moving away from slow, traditional microbial identification algorithms toward rapid molecular methods and mass spectrometry (MS). Historically, MS was clinically utilized as a high-complexity method adapted for protein-centered analysis of samples in chemistry and hematology laboratories. Today, matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) MS is adapted for use in microbiology laboratories, where it serves as a paradigm-shifting, rapid, and robust method for accurate microbial identification. Multiple instrument platforms, marketed by well-established manufacturers, are beginning to displace automated phenotypic identification instruments and in some cases genetic sequence-based identification practices. This review summarizes the current position of MALDI-TOF MS in clinical research and in diagnostic clinical microbiology laboratories and serves as a primer to examine the “nuts and bolts” of MALDI-TOF MS, highlighting research associated with sample preparation, spectral analysis, and accuracy. Currently available MALDI-TOF MS hardware and software platforms that support the use of MALDI-TOF with direct and precultured specimens and integration of the technology into the laboratory workflow are also discussed. Finally, this review closes with a prospective view of the future of MALDI-TOF MS in the clinical microbiology laboratory to accelerate diagnosis and microbial identification to improve patient care.

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Section: Lichtheimia Sppmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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“…C. albieans is the species most often associated with oral lesions, but other species of Candida, including C. glabrata, C. tropiealis and C. parapsilosis have .+ .. also been isolated in the saliva of subjects with and without candidiasis (25,26). Candida has virulence factors that facilitate colonisation and proliferation in the oral mucosa.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Host Defence Mechanisms Induced by Candida Species

Paoletti

Fusco

Grimaldi

et al. 2013

Int J Immunopathol Pharmacol

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Some species of Candida are opportunistic pathogens that can cause disease in a host immunocompromised by underlying local or systemic pathological processes. C. albicans is the species most often associated with oral lesions, but other species of Candida, including C. glabrata, C. tropicalis and C. parapsilosis, have also been isolated in the saliva of subjects with and without candidiasis. In the present study we evaluated the host defence mechanisms induced by Candida albicans and other Candida species in monocytes and oral epithelial cells in order to establish the existence of a species-specific cellular response. Our results indicated that, during Candida species infection, the epithelial cells actively participate in the host defence by producing antimicrobial peptides and proinflammatory cytokines. Moreover, in infections caused by Candida tropicalis and Candida glabrata, the host defence may be strengthened by the release of perforin and granzyme by polymorphonuclear leukocytes recruited at the site of infection.

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“…In addition, investigations to evaluate its potential as a typing tool, including in tracking nosocomial transmission, such as is seen for other fungi (Aspergillus flavus and Candida parapsilosis) [54,55], are warranted.…”

Section: Proteomic Approachesmentioning

confidence: 99%

The Genus Scedosporium and Pseudallescheria: Current Challenges in Laboratory Diagnosis

Ramsperger

Duan

Sorrell

et al. 2014

Curr Clin Micro Rpt

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Scedosporium and Pseudallescheria fungi are increasingly encountered fungal pathogens. Species identification is important as there are differences in epidemiology, virulence, and antifungal susceptibility that vary according to species or species groups. Histological and culture techniques are limited by low sensitivity and specificity but improved culture approaches employing selective media have improved the recovery of these fungi from clinical samples. Molecular-and proteomics-based methods are increasingly explored and used for species identification. These include multiplex polymerase chain reaction (PCR) and other PCR-based methods, oligonucleotide arrays, DNA sequencing, rolling circle amplification, and matrix-assisted laser desorption time-of-flight mass spectrometry. These techniques have been harnessed for the detection and identification of Scedosporium and Pseudallescheria species directly from clinical samples and from cultures. This review summarizes the methods currently used to enable an informed choice of detection and/or identification techniques in the clinical mycology laboratory.

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MALDI-TOF mass spectrometry and microsatellite markers to evaluate Candida parapsilosis transmission in neonatal intensive care units

Cited by 65 publications

References 49 publications

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Assessment of Host Defence Mechanisms Induced by Candida Species

The Genus Scedosporium and Pseudallescheria: Current Challenges in Laboratory Diagnosis

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