MALDI-ToF mass spectrometric analysis for the identification of plague, cholera, and tularemia causative agents

Abstract

General questions of the methodology of Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI ToF MS analysis, a new technology in laboratory diagnostics of infec tions), as well as a number of special questions concerning the use of this technology in the identification and typing of causative agents of extremely dangerous infections (plague, cholera, and tularemia), are considered in the present review. The problems of sample preparation for study and the ensurance of biological safety are discussed.

Tularemia: a re-emerging tick-borne infectious disease

“…Accuracy of this analysis highly depends on available mass spectrum databases. However, MALDI-ToF MS-analysis provides results in accordance with the PCR assay (Afanas’ev et al 2015 ; Karatuna et al 2016 ).…”

Идентификация грибов рода Monilinia при фитосанитарной диагностике

“…Knowing the length of the path of movement of the ion from the ionizer to the detector, as well as the time of this movement, it is possible to calculate the speed of movement of the ion and based on its value to calculate the mass of particles present in the sample, as well as to gene rate a spectrum that characterizes the qualitative composition of the object under study. This composition differs in organisms, which allows comparing the obtained result with the database of the equipment manufacturer [1].…”

“…Зная длину пути перемещения иона от ионизатора до детектора, а также время этого перемещения, можно вычислить скорость движения иона и на основании ее значения рассчитать массу частиц, присутствующих в образце, а также генерировать спектр, характеризующий качественный состав исследуемого объекта. Данный состав отличается у организмов, что позволяет сравнить полученный результат с базой данных производителя оборудования [1].…”