MALDI mass spectrometry imaging of gangliosides in mouse brain using ionic liquid matrix

Chan, Kalok; Lanthier, Patricia; Liu, Xin; Sandhu, Jagdeep K.; Stanimirovic, Danica; Li, Jianjun

doi:10.1016/j.aca.2009.02.051

Cited by 91 publications

(74 citation statements)

References 37 publications

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“…Second, various MALDI matrixes have been tested for finding the best "cold" matrix [3,[6][7][8][9][10]. In several initial reports, 2,5-dihydroxybenzoic acid (DHB) [3,6,8] and 6-aza-2-thiothymine (ATT) [3,6] were found to be reasonably "cold" matrixes for ganglioside detection.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, other engineered matrixes have been reported to perform better than DHB or ATT. Li et al found that an ionic liquid matrix, 1-methylimidazolium α-cyano-4-hydroxycinnamate (ImCHCA) [9], produced much less fragmentation for gangliosides than DHB did [9]. Colsch and Woods also developed a novel matrix mixture, 2,6-dihydroxyacetophenone (DHA)/ammonium sulfate/heptafluorobutyric acid (HFBA), for maximizing ganglioside detection from a complex mixture [1,10].…”

Section: Introductionmentioning

confidence: 99%

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5-Methoxysalicylic Acid Matrix for Ganglioside Analysis with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Lee

Cha

2014

J. Am. Soc. Mass Spectrom.

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Abstract. In this note, we report that high quality ganglioside profiles with minimal loss of sialic acid residues can be obtained in the positive ion mode by using a 5-methoxysalicylic acid (MSA) matrix for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). Our results showed that MSA produced much less sialic acid losses from gangliosides than DHB, although MSA and DHB are differ only by their functional groups at their 5-positions (-OH for DHB and -OCH 3 for MSA). Furthermore, our data also demonstrated that addition of an alkali metal additive was effective for simplifying ganglioside profiles, but not necessary for stabilizing glycosidic bonds of gangliosides if MSA was used as a matrix. This suggests that MALDI MS with MSA has a potential to gain additional benefits from the positive-ion mode analyses without losing performance in ganglioside profiling.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

5-Methoxysalicylic Acid Matrix for Ganglioside Analysis with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Lee

Cha

2014

J. Am. Soc. Mass Spectrom.

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“…There has been increasing interest in the field of lipidomics, which has led to significant research into the analysis and imaging of lipids in tissue sections by MALDI-MS [16,20,24,25,31,34,[46][47][48][49][50][51][52][53][54][55][56][57][58][59]. There is now considerable evidence that lipids are differentially expressed in normal and diseased tissue, and that they may play a central role in the pathologic processes associated with numerous disease states, including diabetes, Alzheimer's and dementia, cancer, heart disease, and numerous metabolic diseases [25,50,52,58,[60][61][62][63][64][65][66][67][68][69].…”

Section: Introductionmentioning

confidence: 99%

Imaging of Phospholipids in Formalin Fixed Rat Brain Sections by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry

Carter

McLeod

Bunch

2011

J. Am. Soc. Mass Spectrom.

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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a valuable tool for the analysis of molecules directly from tissue. Imaging of phospholipids is gaining widespread interest, particularly as these lipids have been implicated in a variety of pathologic processes. Formalin fixation (FF) is the standard protocol used in histology laboratories worldwide to preserve tissue for analysis, in order to aid in the diagnosis and prognosis of diseases. This study assesses MALDI imaging of phospholipids directly in formalin fixed tissue, with a view to future analysis of archival tissue. This investigation proves the viability of MALDI-MSI for studying the distribution of lipids directly in formalin fixed tissue, without any pretreatment protocols. High quality molecular images for several phosphatidylcholine (PC) and sphingomyelin (SM) species are presented. Images correspond well with previously published data for the analysis of lipids directly from freshly prepared tissue. Different ionization pathways are observed when analyzing fixed tissue compared with fresh, and this change was found to be associated with formalin buffers employed in fixation protocols. The ability to analyze lipids directly from formalin fixed tissue opens up new doors in the investigation of disease profiles. Pathologic specimens taken for histologic investigation can be analyzed by MALDI-MS to provide greater information on the involvement of lipids in diseased tissue.

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“…They introduced a new ionic liquid matrix that was capable of identifying gangliosides from mouse brain tissue sections without dissociation of sialic acid during the ionization process. Using IMS experiments, they successfully showed the distribution of GM1 at m/z 1546 and m/z 1574 on the pyramidal neuronal cell layer and the dentate gyrus region, respectively (Chan et al, 2009). These regions play an important role in learning and memory formation (Shors et al, 2001) and are affected during neurodegenerative diseases such as Alzheimer's disease (Jeyaku-mar et al, 2005).…”

Section: Metabolomics and Its Perspectives: Lipidsmentioning

confidence: 99%

Application of MALDI Tissue Imaging of Drugs and Metabolites: A New Frontier for Molecular Histology

Shanta¹,

Kim²,

Kim³

et al. 2011

Biomolecules and Therapeutics

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MALDI mass spectrometry imaging of gangliosides in mouse brain using ionic liquid matrix

Cited by 91 publications

References 37 publications

5-Methoxysalicylic Acid Matrix for Ganglioside Analysis with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

5-Methoxysalicylic Acid Matrix for Ganglioside Analysis with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Imaging of Phospholipids in Formalin Fixed Rat Brain Sections by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry

Application of MALDI Tissue Imaging of Drugs and Metabolites: A New Frontier for Molecular Histology

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