MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma

Abstract

Reducing the incidence and mortality rates for clear cell renal cell carcinoma (ccRCC) remains a significant clinical challenge with poor 5-year survival rates. A unique tissue cohort was assembled of matched ccRCC and distal non-tumor tissues (n=20) associated with moderate risk of disease progression, half of these from individuals who progressed to metastatic disease and the other half who remained disease free. These tissues were used for MALDI imaging mass spectrometry profiling of proteins in the 2–20 kDa range, resulting in a panel of 108 proteins that had potential disease specific expression patterns. Protein lysates from the same tissues were analyzed by tandem mass spectrometry, resulting in identification of 56 proteins of less than 20 kDa molecular weight. The same tissues were also used for global lipid profiling analysis by MALDI-FTICR mass spectrometry. From the cumulative protein and lipid expression profile data, a refined panel of 26 proteins and 39 lipid species were identified that could either distinguish tumor from non-tumor tissues, or tissues from recurrent disease progressors from non-recurrent disease individuals. This approach has the potential to not only improve prognostic assessment and enhance post-operative surveillance, but also to inform on the underlying biology of ccRCC progression.

Lipidomic differentiation between human kidney tumors and surrounding normal tissues using HILIC-HPLC/ESI–MS and multivariate data analysis

“…Mass spectrometry imaging using matrix-assisted laser desorption/ionization or desorption electrospray ionization was used for the relative comparison of tumor and surrounding normal tissues [19][20][21][22]. Increased absolute intensities for PI 18:0/20:4, PS 18:0/18:1 and PI 22:4/18:0 were reported [19,21] in kidney tumor tissues using the desorption electrospray ionization imaging.…”

A MALDI-MSI Approach to the Characterization of Radiation-Induced Lung Injury and Medical Countermeasure Development

“…This molecular imaging technique has been applied to the investigation of lipids, metabolites and proteins in a plethora of pathophysiological processes(Carter et al 2011, Palmer et al 2012, Steven and Bunch 2013, Berry et al 2014, Djidja et al 2014, Jackson et al 2014, Jones et al 2014, Manier et al 2014, Seeley et al 2014, Shariatgorji et al 2014). By applying mass spectrometry to region-specific anatomical tissue architecture, MALDI-MSI offers another dimension in characterizing the natural history and development of these pathologies.…”

Renal Glycosphingolipid Metabolism Is Dysfunctional in Lupus Nephritis

“…40,41 Relative concentrations of GlcCer and LacCer species were assessed simultaneously in the same sections by MALDI-IMS in frozen kidney sections. Kidney tissues (10 mm) were prepared on ITO-coated glass slides, rinsed in water, and desiccated.…”