“MALDI-FLIP-on-a-chip” and “MALDI-FRAP-on-a-flap”: Novel Techniques for Soil Microbiology and Environmental Biogeochemistry. II – Polymer Chip Prototyping (Invited Paper)

Abstract

In the second part of the article cycle «"MALDI-FLIP-on-a-chip" and "MALDI-FRAP-on-aflap": Novel Techniques for Soil Microbiology and Environmental Biogeochemistry» the polymer chip prototyping techniques are described. In particular, the compatibility problems are discussed. The problem of the biocompatibility of polymer chip surface is eliminated using GIM-surfaced chip exposition in the high-humidity experimental media, which may be interpreted as a model of soils. The problem of compatibilities of fluorescence techniques and polymer chips is resolved (as a part of the general chip optics problem) using microscopic investigations of polymer chip transparency in some different textural variances and microfluorimetric measurements of fluorescent dyes in the chip geometry. The problem of the soil chip prototyping is solved using 3D-printing based on some biocompatible and, so possible, biodegradable polymers. The basic complexity of experimental data is provided in the tables placed in the general article text. Is it possible to create multiparametric analytical technique for synchronous biocompatible soil microbiome analysis and monitoring? It is a general question for the real time environmental control. We can say "Yes", but only if we have a minimal prerequisite case, which we have a good polymer, real "real time" analyzer, biocompatible and biodegradable coatings etc. In other cases the general problem of soil chip design is not a problem of engineering, but it is a problem of soil-chip interface chemical physics and physical chemistry. Such problem may be interpreted only as a principal physical, but not as a technical problem. This part of our article cycle is very simple and sensible, because such problems are not very strong and complexible, consequently, we can take only illustration of principle, but not a full verification and validation of fine and thin mechanisms of soil microbiome interactions with adhesive chip etc. Cureent opinions in soil biology are good compartible with our results, therefor we must not take full theoretical considerations in frames of the current concept paradigm in soil biology. De facto, it is a brief methodical and technical note before the closing of our projects in Russian Federation. It is not a normal research article, because all normal articles may be writed only in normal material and technical conditions.