Malate Synthesis and Secretion Mediated by a Manganese-Enhanced Malate Dehydrogenase Confers Superior Manganese Tolerance in <i>Stylosanthes guianensis</i>

Chen, Zhijian; Sun, Lili; Liu, Pandao; Liu, Guodao; Tian, Jichun; Liao, Hong

doi:10.1104/pp.114.251017

Cited by 102 publications

(93 citation statements)

References 51 publications

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“…Under the same conditions, roots of atalmt1 mutants continue to grow normally, because the absence of malate efflux prevents Fe 3+ from accumulating in the apoplast. Some findings in this study appear to contradict Chen et al (2015), who reported that the addition of external malate increased Mn tolerance in yeast (Saccharomyces cerevisiae) and Stylosanthes guianensis, a forage legume species. That study concluded that part of the genotypic variation for Mn tolerance in S. guianensis was attributable to greater malate efflux from roots.…”

Section: Attempts To Mimic the Ox Phenotype In Wild-type Plants With contrasting

confidence: 55%

“…Interestingly, the authors mentioned that the ameliorative effects of malate became smaller or even disappeared when malate concentrations were raised (Chen et al, 2015). Therefore, it is possible that even higher concentrations of malate would have increased the toxicity of Mn, as we described here.…”

Section: Attempts To Mimic the Ox Phenotype In Wild-type Plants With mentioning

confidence: 65%

“…However, important differences exist between the two experimental systems that could explain our contrasting conclusions. Chen et al (2015) conducted much shorter growth assays on solid agar medium and used very different ratios of malate:Mn. They found that 10 mM malate ameliorated the inhibition of S. guianensis growth in 20 mM Figure 11.…”

Section: Attempts To Mimic the Ox Phenotype In Wild-type Plants With mentioning

confidence: 99%

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Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

et al. 2017

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ORCID IDs: 0000-0003-1555-4754 (J.L.); 0000-0003-3009-7854 (M.Z.); 0000-0002-1376-9543 (P.R.R.).Aluminum-activated malate transporters (ALMTs) form a family of anion channels in plants, but little is known about most of its members. This study examined the function of OsALMT4 from rice (Oryza sativa). We show that OsALMT4 is expressed in roots and shoots and that the OsALMT4 protein localizes to the plasma membrane. Transgenic rice lines overexpressing (OX) OsALMT4 released malate from the roots constitutively and had 2-fold higher malate concentrations in the xylem sap than nulls, indicating greater concentrations of malate in the apoplast. OX lines developed brown necrotic spots on the leaves that did not appear on nulls. These symptoms were not associated with altered concentrations of any mineral element in the leaves, although the OX lines had higher concentrations of Mn and B in their grain compared with nulls. While total leaf Mn concentrations were not different between the OX and null lines, Mn concentrations in the apoplast were greater in the OX plants. The OX lines also displayed increased expression of Mn transporters and were more sensitive to Mn toxicity than null plants. We showed that the growth of wild-type rice was unaffected by 100 mM Mn in hydroponics but, when combined with 1 mM malate, this concentration inhibited growth. We conclude that increasing OsALMT4 expression affected malate efflux and compartmentation within the tissues, which increased Mn concentrations in the apoplast of leaves and induced the toxicity symptoms. This study reveals new links between malate transport and mineral nutrition.

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Section: Attempts To Mimic the Ox Phenotype In Wild-type Plants With contrasting

confidence: 55%

Section: Attempts To Mimic the Ox Phenotype In Wild-type Plants With mentioning

confidence: 65%

Section: Attempts To Mimic the Ox Phenotype In Wild-type Plants With mentioning

confidence: 99%

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Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

et al. 2017

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“…Excess Mn can induce brown spots on mature leaves5, interveinal chlorosis, and necrotic and deformed young leaves6. Some plants that adapt well to acid soils may have specific strategies that give them a superior ability to tolerate Mn toxicity3541. After 14 d of treatment, leaf chlorosis was observed in ‘YS’ when cultured in 250 μM MnSO 4 , but not in ‘LJCY’ (Fig.…”

Section: Discussionmentioning

confidence: 99%

Metal transport protein 8 in Camellia sinensis confers superior manganese tolerance when expressed in yeast and Arabidopsis thaliana

et al. 2017

Sci Rep

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Manganese (Mn) is an important micronutrient element required for plant growth and development, playing catalytic roles in enzymes, membranes and DNA replication. The tea plant (Camellia sinensis) is able to accumulate high concentration of Mn without showing signs of toxicity, but the molecular mechanisms underlying this remain largely unknown. In this study, the C. sinensis cultivar ‘LJCY’ had higher Mn tolerance than cultivar ‘YS’, because chlorophyll content reduction was lower under the high Mn treatment. Proteomic analysis of the leaves revealed that C. sinensis Metal Tolerance Protein 8 (CsMTP8) accumulated in response to Mn toxicity in cultivar ‘LJCY’. The gene encoding CsMTP8, designated as CsMTP8 was also isolated, and its expression enhanced Mn tolerance in Saccharomyces cerevisiae. Similarly, the overexpression of CsMTP8 in Arabidopsis thaliana increased plant tolerance and reduced Mn accumulation in plant tissues under excess Mn conditions. Subcellular localization analysis of green florescence fused protein indicated that CsMTP8 was localized to the plasma membranes. Taken together, the results suggest that CsMTP8 is a Mn-specific transporter, which is localized in the plasma membrane, and transports excess Mn out of plant cells. The results also suggest that it is needed for Mn tolerance in shoots.

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“…Therefore, Mn is necessary for plant growth and development (Chen et al, 2015). However, excess Mn is toxic to plants.…”

Section: Introductionmentioning

confidence: 99%

Manganese Toxicity Inhibited Root Growth by Disrupting Auxin Biosynthesis and Transport in Arabidopsis

et al. 2017

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Mn toxicity inhibits both primary root (PR) growth and lateral root development. However, the mechanism underlying Mn-mediated root growth inhibition remains to be further elucidated. Here, we investigated the role of auxin in Mn-mediated inhibition of PR growth in Arabidopsis using physiological and genetic approaches. Mn toxicity inhibits PR elongation by reducing meristematic cell division potential. Mn toxicity also reduced auxin levels in root tips by reducing IAA biosynthesis and down-regulating the expression of auxin efflux carriers PIN4 and PIN7. Loss of function pin4 and pin7 mutants showed less inhibition of root growth than col-0 seedlings. These results indicated that this inhibitory effect of Mn toxicity on PR growth was mediated by affecting auxin biosynthesis and the expression of auxin efflux transporters PIN4 and PIN7.

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Malate Synthesis and Secretion Mediated by a Manganese-Enhanced Malate Dehydrogenase Confers Superior Manganese Tolerance in Stylosanthes guianensis

Cited by 102 publications

References 51 publications

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

Metal transport protein 8 in Camellia sinensis confers superior manganese tolerance when expressed in yeast and Arabidopsis thaliana

Manganese Toxicity Inhibited Root Growth by Disrupting Auxin Biosynthesis and Transport in Arabidopsis

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