Malassezia pachyderznatis: properties and storage

Abstract

Summary. Five strains of Malassezia pachydermatis isolated from small animals in Bratislava were subcultured, maintained under sterile paraffin oil and preserved in liquid nitrogen. Morphological and physiological characteristics as well as the analysis of long chain fatty acids of the cell mass were determined. The results showed that the maintenance in liquid nitrogen is the most satisfactory method of preservation of pure cultures of Malassezia pachydermatis. Zusammenfassung. Fünf Stämme der Art Ma‐lassezia pachydermatis wurden von verschiedenen Kleintieren in Bratislava isoliert, subkultiviert und unter Paraffinöl und in flüssigem Stickstoff aufbe‐wahrt. Die morphologischen und physiologischen Eigenschaften sowie auch die langkettigen Fettsäu‐ren in der Zellmasse wurden untersucht. Die Re‐sultate zeigen, daß die Aufbewahrung im flüssigen Stickstoff die beste Methode für die Stammhaltung von Malassezia pachydermatis ist, die bis jetzt nur sehr schwer in Sammlungen zu halten war.

Malassezia pachydermatis: a review

“…Cells survive lyophilization [2,51]. Preservation in liquid nitrogen is also efficient [51,52]. Lorenzini & de Bernardis [53] suggested the use of the glucose nutrient agar supplemented with 1·5% yeast extract and 1% Tween 80; cultures maintained at room temperature were kept alive for at least 3 months.…”

“…Numerous protein bands ranging from 15·0–145·7 kDa were detected, but profiles did vary according to the origin of the strains (external ear canal or skin). Breierova et al [52] demonstrated that the content of unsaturated acids (oleic, linoleic, myristoleic and palmitoleic) was 2·5–7·5 times greater than that of saturated acids (palmitic, stearic and myristic). Great variations were noticed between strains.…”

Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis

“…As in other yeasts, the cytoplasmic microtubules, spindle and astral microtubules are probably involved in positioning, migration and division of the nucleus (Banuett and Herskowitz, 2002). Migration of the nucleus to the bud before mitosis, also observed at the ultrastructural level, gives support to inclusion of the Malassezia genus into the fungal group basidiomycota Breierová et al, 1991;Guillot et al, 1995;Guého et al, 1996). The distribution of microtubules during the cell cycle of M. pachydermatis was found to be similar to that in other basidiomycetous yeasts (Slaninová et al, 1999;Kopecká et al, 2001b;Banuett and Herskowitz, 2002;Straube et al, 2003).…”