Malaria vectors of Papua New Guinea

Cooper, Robert D.; Waterson, D. G. E.; Frances, Stephen P.; Beebe, Nigel W.; Pluess, Bianca; Sweeney, A. W.

doi:10.1016/j.ijpara.2009.05.009

Cited by 58 publications

(96 citation statements)

References 32 publications

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“…al. 2000), Senegal (Lochouarn and Fontenille, 1999), and Papua New Guinea (Cooper et al, 2009) (Burkot et al, 1984;Wirtz et al, 1985;Wirtz et al, 1992). Our results confirm that the application of CSP-ELISA alone is risky in vector identification because false positives occur more frequently in zoophilic species, which are less likely to be malaria vectors.…”

Section: Discussionsupporting

confidence: 67%

False positivity of circumsporozoite protein (CSP)–ELISA in zoophilic anophelines in Bangladesh

et al. 2013

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Circumsporozoite protein enzyme-linked immunosorbent assays (CSP-ELISA) are widely used for malaria vector identification throughout the world. However, several studies have reported false-positive results when using this method. The present study was conducted to estimate the frequency of false positives among anopheline species in malaria endemic areas of Bangladesh. In total, 4,724 Anopheles females belonging to 25 species were collected and tested for Plasmodium falciparum, Plasmodium vivax-210, and P. vivax-247 CSP. Initially, 144 samples tested positive using routine CSP-ELISA, but the number of positive results declined to 85 (59%) when the samples were tested after heating at 100 0 C for 10 min to remove false-positive specimens.

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Section: Discussionsupporting

confidence: 67%

False positivity of circumsporozoite protein (CSP)–ELISA in zoophilic anophelines in Bangladesh

et al. 2013

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“…Relapse can thus contribute to greater complexity of infection and higher levels of parasite diversity and may also increase gene flow through human movement and help to maintain larger, more stable parasite populations than P. falciparum. 23,87,88 This survey was conducted as a baseline to future surveys monitoring the success of the PNG national malaria control program with the first countrywide LLIN distribution completed in the year of the survey (2008). Since then, Plasmodium spp.…”

Section: Discussionmentioning

confidence: 99%

Higher Complexity of Infection and Genetic Diversity of Plasmodium vivax Than Plasmodium falciparum across all Malaria Transmission Zones of Papua New Guinea

et al. 2017

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Abstract. Plasmodium falciparum and Plasmodium vivax have varying transmission dynamics that are informed by molecular epidemiology. This study aimed to determine the complexity of infection and genetic diversity of P. vivax and P. falciparum throughout Papua New Guinea (PNG) to evaluate transmission dynamics across the country. In 2008-2009, a nationwide malaria indicator survey collected 8,936 samples from all 16 endemic provinces of PNG. Of these, 892 positive P. vivax samples were genotyped at PvMS16 and PvmspF3, and 758 positive P. falciparum samples were genotyped at Pfmsp2. The data were analyzed for multiplicity of infection (MOI) and genetic diversity. Overall, P. vivax had higher polyclonality (71%) and mean MOI (2.32) than P. falciparum (20%, 1.39). These measures were significantly associated with prevalence for P. falciparum but not for P. vivax. The genetic diversity of P. vivax (PvMS16: expected heterozygosity = 0.95, 0.85-0.98; PvMsp1F3: 0.78, 0.66-0.89) was higher and less variable than that of P. falciparum (Pfmsp2: 0.89, 0.65-0.97). Significant associations of MOI with allelic richness (rho = 0.69, P = 0.009) and expected heterozygosity (rho = 0.87, P < 0.001) were observed for P. falciparum. Conversely, genetic diversity was not correlated with polyclonality nor mean MOI for P. vivax. The results demonstrate higher complexity of infection and genetic diversity of P. vivax across the country. Although P. falciparum shows a strong association of these parameters with prevalence, a lack of association was observed for P. vivax and is consistent with higher potential for outcrossing of this species.

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“…The specificity of the assay was then demonstrated by using probes identified in Figure 1 and Table 2 to differentiate PCR products amplified from previously sequenced species-specific controls. Because members of the Punctulatus group are known to carry Plasmodium 19,28,29 and Wuchereria parasites 13 and human blood meals, 3,28 we included six additional controls containing Plasmodium falciparum , P. vivax , P. malariae , P. ovale , Wuchereria bancrofti , and human genomic DNA.…”

Section: Its2 Sequence Analysis and Post-pcr-ldr-fma Assay Design Prmentioning

confidence: 99%

“…punctulatus . 3,13,17,19 Given this background, among the 340 mosquitoes subjected to ITS2 LDR-FMA analysis, morphological assessment identified 67 as An. punctulatus , 115 as An.…”

Section: Its2 Sequence Analysis and Post-pcr-ldr-fma Assay Design Prmentioning

confidence: 99%

“…punctulatus as the primary vectors of malaria and filarial parasites. 13,18,19 However, given the heterogeneous distribution of An. punctulatus species complex members across Papua New Guinea, in addition to what is known about the variable breeding and biting preferences among species 3,17,20 it will be important to understand if all members of the Punctulatus group transmit parasites, or if there is further heterogeneity among species in their competence to transmit parasites.…”

Section: Introductionmentioning

confidence: 99%

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High Throughput Multiplex Assay for Species Identification of Papua New Guinea Malaria Vectors: Members of the Anopheles punctulatus (Diptera: Culicidae) Species Group

Halldin¹,

Reimer²,

Thomsen³

et al. 2011

The American Journal of Tropical Medicine and Hygiene

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Malaria vectors of Papua New Guinea

Cited by 58 publications

References 32 publications

False positivity of circumsporozoite protein (CSP)–ELISA in zoophilic anophelines in Bangladesh

False positivity of circumsporozoite protein (CSP)–ELISA in zoophilic anophelines in Bangladesh

Higher Complexity of Infection and Genetic Diversity of Plasmodium vivax Than Plasmodium falciparum across all Malaria Transmission Zones of Papua New Guinea

High Throughput Multiplex Assay for Species Identification of Papua New Guinea Malaria Vectors: Members of the Anopheles punctulatus (Diptera: Culicidae) Species Group

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