“…The studies based on nucleic approach are seriously affected when the RBCs become really nucleated, such as when the RBCs lifespan is near the end or the RBCs are highly matured. The nucleic approach is followed by Kumarasamy, Ong, and Tan (), Khawaldeh (), and Zou, Chen, Zhang, and Garcia () in the segmentation of RBCs. The segmentation based on chromatin dots offers no surety that on the basis of maximum and minimum intensity levels that they will be the same in all images and in addition, these studies are highly susceptible to noise (Rehman, Kurniawan, & Saba, ; Saba, Rehman, Al‐Dhelaan, & Al‐Rodhaan, ).…”