Making sense of quorum sensing in lactobacilli: a special focus on Lactobacillus plantarum WCFS1

Sturme, Mark H. J.; Francke, Christof; Siezen, Roland J.; Vos, Willem M. de; Kleerebezem, Michiel

doi:10.1099/mic.0.2007/012831-0

Cited by 77 publications

(61 citation statements)

References 58 publications

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“…Environmental adaptation. Optimal bacterial adaptation to an environment is strongly associated with the expression of a series of sensors/regulators (38,39). The L. casei genome contains 16 complete TCS and 124 transcriptional regulators contributing to its ability to sense and adapt to various environments (37).…”

Section: Significancementioning

confidence: 99%

Functional genomics of Lactobacillus casei establishment in the gut

Licandro

Scornec

Pédron

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Although the composition of the gut microbiota and its symbiotic contribution to key host physiological functions are well established, little is known as yet about the bacterial factors that account for this symbiosis. We selected Lactobacillus casei as a model microorganism to proceed to genomewide identification of the functions required for a symbiont to establish colonization in the gut. As a result of our recent development of a transposonmutagenesis tool that overcomes the barrier that had prevented L. casei random mutagenesis, we developed a signature-tagged mutagenesis approach combining whole-genome reverse genetics using a set of tagged transposons and in vivo screening using the rabbit ligated ileal loop model. After sequencing transposon insertion sites in 9,250 random mutants, we assembled a library of 1,110 independent mutants, all disrupted in a different gene, that provides a representative view of the L. casei genome. By determining the relative quantity of each of the 1,110 mutants before and after the in vivo challenge, we identified a core of 47 L. casei genes necessary for its establishment in the gut. They are involved in housekeeping functions, metabolism (sugar, amino acids), cell wall biogenesis, and adaptation to environment. Hence we provide what is, to our knowledge, the first global functional genomics analysis of L. casei symbiosis.commensalism | Lactic acid bacteria T he pioneering studies that led to the characterization of the gut microbiota were reviewed in 2001 (1). These studies and recent investigations have revealed mutualistic functions (2), including a barrier effect against allogenic microbes (3), fermentation of complex sugars (4, 5), and maturation and homeostasis of the immune system (6). Recent metagenomic studies have revealed an extraordinary diversity of genes constituting the gut microbiome (7), opening the way to correlative studies linking microbiome diversity, homeostasis, and diseases (5,8,9).In parallel, some representative species, i.e., "model symbionts," now are being studied functionally (10). As it was done for pathogens, it is essential to develop the cellular microbiology of symbionts and particularly to identify the genes required for their establishment and persistence in the gut. Transcriptomic profiling identified up-regulated genes linked to metabolic functions, stress responses, and pili synthesis during early colonization (11-13). Comparative genomics among Lactobacilli identified strain-specific candidate genes for extended colonization: In Lactobacillus rhamnosus, persistence was attributed to an spaCBA locus encoding LPXTG-like pilins (14), and in Lactobacillus johnsonii it was attributed to specific glycosyltransferases, a phosphotransfer system, and a protease (15). Otherwise, a functional in vivo screening based on the expression of a genomic library of Bacteroides fragilis identified a locus encoding polysaccharide utilization as essential for stable colonization of murine colonic crypts (16). Alternatively, colonization of germ-free mic...

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Section: Significancementioning

confidence: 99%

Functional genomics of Lactobacillus casei establishment in the gut

Licandro

Scornec

Pédron

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Interestingly, the ⌬lamA mutant was able to produce 50% of the LamD558 peptide produced by the wild type and 30% of the transcripts of the lamBDCA operon produced by the wild type, suggesting that there was cross-regulation with other genes (4). The genome information revealed that 13 putative 2CSs were present in this strain, and 5 of them were predicted to be involved in quorum sensing, as deduced from their sequences, as previously reported (19,22,35,36). In addition, the genes encoding one of these 2CSs, lp_3088 and lp_3087 (hpk10 and rrp10), are highly homologous to lamC and lamA, respectively.…”

mentioning

confidence: 68%

Two Homologous Agr-Like Quorum-Sensing Systems Cooperatively Control Adherence, Cell Morphology, and Cell Viability Properties in Lactobacillus plantarum WCFS1

et al. 2008

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A two-component regulatory system of Lactobacillus plantarum, encoded by genes designated lamK and lamR (hpk10 and rrp10), was studied. The lamK and lamR genes encode proteins which are highly homologous to the quorum-sensing histidine kinase LamC and the response regulator LamA, respectively. Transcription analysis of the lamKR operon and the lamBDCA operon and liquid chromatography-mass spectrometry analysis of production of the LamD558 autoinducing peptide were performed for ⌬lamA, ⌬lamR, ⌬lamA ⌬lamR deletion mutants and a wild-type strain. The results suggested that lamA and lamR are cooperating genes. In addition, typical phenotypes of the ⌬lamA mutant, such as reduced adherence to glass surfaces and filamentous cell morphology, were enhanced in the ⌬lamA ⌬lamR mutant. Microarray analysis suggested that the same cell wall polysaccharide synthesis genes, stress response-related genes, and cell wall protein-encoding genes were affected in the ⌬lamA and ⌬lamA ⌬lamR mutants. However, the regulation ratio was more significant for the ⌬lamA ⌬lamR mutant, indicating the cooperative effect of LamA and LamR.

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“…It represented the beginning of the stationary phase of growth in FLPB-1 and coincided with the greater cellular density period. Several studies have shown that the antimicrobial compounds production, such as bacteriocins, are dependent on high biomass in the medium because this is an important factor related to signalization of physiological responses able to active operons required for the synthesis, transport and regulations these compounds in LABs (Maldonado et al, 2003;Sturme et al, 2007).…”

Section: Resultsmentioning

confidence: 99%