Making a Pest Beneficial: Fungus Gnats [Bradysia impatiens (Diptera: Sciaridea)] as Potential Vectors of Microbial Control Agents to Suppress Pathogens they Also Spread

Kapongo, Jean-Pierre; Kevan, Peter G.; Shipp, Les; Taki, Hisatomo

doi:10.1007/978-3-030-18917-4_13

Cited by 1 publication

(1 citation statement)

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“…Nevertheless, a few reports have indicated the role of adults of B. impatiens in pathogen transmission [ 12 ]. Kapongo and co-workers in 2020 showed that not only larva, but also adults of B. impatiens might be a vector of Fusarium oxysporum and Pythium aphanidermatum [ 35 ]. In this study, we have not confirmed the ability of an adult insect to transmit the virus to healthy plants.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Role of Bradysia impatiens (Diptera: Sciaridae) as a Vector Transmitting Peanut Stunt Virus on the Model Plant Nicotiana benthamiana

Budziszewska

Frąckowiak

Obrępalska‐Stęplowska

2021

Cells

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Bradysia species, commonly known as fungus gnats, are ubiquitous in greenhouses, nurseries of horticultural plants, and commercial mushroom houses, causing significant economic losses. Moreover, the insects from the Bradysia genus have a well-documented role in plant pathogenic fungi transmission. Here, a study on the potential of Bradysia impatiens to acquire and transmit the peanut stunt virus (PSV) from plant to plant was undertaken. Four-day-old larvae of B. impatiens were exposed to PSV-P strain by feeding on virus-infected leaves of Nicotiana benthamiana and then transferred to healthy plants in laboratory conditions. Using the reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR (RT-qPCR), and digital droplet PCR (RT-ddPCR), the PSV RNAs in the larva, pupa, and imago of B. impatiens were detected and quantified. The presence of PSV genomic RNA strands as well as viral coat protein in N. benthamiana, on which the viruliferous larvae were feeding, was also confirmed at the molecular level, even though the characteristic symptoms of PSV infection were not observed. The results have shown that larvae of B. impatiens could acquire the virus and transmit it to healthy plants. Moreover, it has been proven that PSV might persist in the insect body transstadially. Although the molecular mechanisms of virion acquisition and retention during insect development need further studies, this is the first report on B. impatiens playing a potential role in plant virus transmission.

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Section: Discussionmentioning

confidence: 99%