Make engineered living materials carry their weight

Heveran, Chelsea M.; Hernandez, Christopher J.

doi:10.1016/j.matt.2023.07.023

Cited by 6 publications

(3 citation statements)

References 102 publications

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“…Some studies have created bulk materials by utilizing the natural expression and secretion of structural proteins in microorganisms and their interactions . Various research efforts have introduced assembly concepts to strengthen these protein interactions, achieving more robust bulk materials . Nevertheless, further in-depth research is needed to advance understanding and development in achieving the desired structure and rigidity.…”

Section: Fabrication Of Elmsmentioning

confidence: 99%

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Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Lu,

Huang,

Cui

et al. 2024

ACS Synth. Biol.

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Engineered Living Materials (ELMs) are materials composed of or incorporating living cells as essential functional units. These materials can be created using bottom-up approaches, where engineered cells spontaneously form welldefined aggregates. Alternatively, top-down methods employ advanced materials science techniques to integrate cells with various kinds of materials, creating hybrids where cells and materials are intricately combined. ELMs blend synthetic biology with materials science, allowing for dynamic responses to environmental stimuli such as stress, pH, humidity, temperature, and light. These materials exhibit unique "living" properties, including self-healing, self-replication, and environmental adaptability, making them highly suitable for a wide range of applications in medicine, environmental conservation, and manufacturing. Their inherent biocompatibility and ability to undergo genetic modifications allow for customized functionalities and prolonged sustainability. This review highlights the transformative impact of ELMs over recent decades, particularly in healthcare and environmental protection. We discuss current preparation methods, including the use of endogenous and exogenous scaffolds, living assembly, 3D bioprinting, and electrospinning. Emphasis is placed on ongoing research and technological advancements necessary to enhance the safety, functionality, and practical applicability of ELMs in real-world contexts.

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Section: Fabrication Of Elmsmentioning

confidence: 99%

“…10 Various research efforts have introduced assembly concepts to strengthen these protein interactions, achieving more robust bulk materials. 225 Nevertheless, further in-depth research is needed to advance understanding and development in achieving the desired structure and rigidity.…”

Section: Fabrication Of Elmsmentioning

confidence: 99%

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Lu,

Huang,

Cui

et al. 2024

ACS Synth. Biol.

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“…In the past 2 decades, a plethora of methods and technologies have been established to achieve precise patterning of bacteria for diverse applications. − By using “patterning of bacteria” and “biofilm” as the keywords and restricting the search to the period of “2004–2023” on the Web of Science, more than 4000 publications have been retrieved (as of December 31, 2023), suggesting the rapid development of bacterial patterning during this time frame. , However, there are no relevant review articles comprehensively introducing the technologies of bacterial patterning. To fill this gap, this mini-review aims to provide a systematic summary of bacterial patterning technologies and their applications in the biomedical field.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Patterning: A Promising Biofabrication Technique

Xiao,

Lv,

Zhu

2024

ACS Appl. Bio Mater.

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Engineering Microbial Consortia as Living Materials: Advances and Prospectives

Wang,

Zhan,

Jiang

et al. 2024

ACS Synth. Biol.

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The field of Engineered Living Materials (ELMs) integrates engineered living organisms into natural biomaterials to achieve diverse objectives. Multiorganism consortia, prevalent in both naturally occurring and synthetic microbial cultures, exhibit complex functionalities and interrelationships, extending the scope of what can be achieved with individual engineered bacterial strains. However, the ELMs comprising microbial consortia are still in the developmental stage. In this Review, we introduce two strategies for designing ELMs constituted of microbial consortia: a top-down strategy, which involves characterizing microbial interactions and mimicking and reconstructing natural ecosystems, and a bottom-up strategy, which entails the rational design of synthetic consortia and their assembly with material substrates to achieve user-defined functions. Next, we summarize technologies from synthetic biology that facilitate the efficient engineering of microbial consortia for performing tasks more complex than those that can be done with single bacterial strains. Finally, we discuss essential challenges and future perspectives for microbial consortia-based ELMs.

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Make engineered living materials carry their weight

Cited by 6 publications

References 102 publications

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Toward Practical Applications of Engineered Living Materials with Advanced Fabrication Techniques

Bacterial Patterning: A Promising Biofabrication Technique

Engineering Microbial Consortia as Living Materials: Advances and Prospectives

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