Make Conjugation Simple: A Facile Approach to Integrated Nanostructures

Xu, Yaolin; Palchoudhury, Soubantika; Qin, Ying; Macher, Thomas; Bao, Yuping

doi:10.1021/la301200g

Cited by 48 publications

(61 citation statements)

References 43 publications

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“…Iron oxide NPs were synthesized using our previously published method by decomposing the iron-oleate complex over 300°C in the presence of oleic acid and trioctylphosphine oxide (Palchoudhury et al, 2011a(Palchoudhury et al, , 2011b(Palchoudhury et al, , 2011cXu et al, 2011Xu et al, , 2012. The as-synthesized NPs were transferred from organic solvents to aqueous solution using a ligand exchange method (Xu et al, 2011(Xu et al, , 2014, where original hydrophobic coatings were replaced with hydrophilic ligands.…”

Section: Discussionmentioning

confidence: 99%

The responses of immune cells to iron oxide nanoparticles

Sherwood

Lackey

et al. 2016

J of Applied Toxicology

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Immune cells play an important role in recognizing and removing foreign objects, such as nanoparticles. Among various parameters, surface coatings of nanoparticles are the first contact with biological system, which critically affect nanoparticle interactions. Here, surface coating effects on nanoparticle cellular uptake, toxicity and ability to trigger immune response were evaluated on a human monocyte cell line using iron oxide nanoparticles. The cells were treated with nanoparticles of three types of coatings (negatively charged polyacrylic acid, positively charged polyethylenimine and neutral polyethylene glycol). The cells were treated at various nanoparticle concentrations (5, 10, 20, 30, 50 μg ml À1 or 2, 4, 8, 12, 20 μg cm À2 ) with 6 h incubation or treated at a nanoparticle concentration of 50 μg ml À1 (20 μg cm À2 ) at different incubation times (6, 12, 24, 48 or 72 h). Cell viability over 80% was observed for all nanoparticle treatment experiments, regardless of surface coatings, nanoparticle concentrations and incubation times. The much lower cell viability for cells treated with free ligands (e.g.~10% for polyethylenimine) suggested that the surface coatings were tightly attached to the nanoparticle surfaces. The immune responses of cells to nanoparticles were evaluated by quantifying the expression of toll-like receptor 2 and tumor necrosis factor-α. The expression of tumor necrosis factor-α and toll-like receptor 2 were not significant in any case of the surface coatings, nanoparticle concentrations and incubation times. These results provide useful information to select nanoparticle surface coatings for biological and biomedical applications.

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Section: Discussionmentioning

confidence: 99%

The responses of immune cells to iron oxide nanoparticles

Sherwood

Lackey

et al. 2016

J of Applied Toxicology

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“…Integration of multiple nanocomponents provides the capability of performing multiple tasks on a single platform [60-62]. For example, the integration of magnetic nanoparticles with fluorescent probes offer dual imaging probes [63] [64], and magnetic nanoparticle-quantum dot hybrid systems allow simultaneous multimodal imaging (microscopy, fluorescence, MRI) [65, 66] and therapy (hyperthermia) [67, 68]. …”

Section: Introductionmentioning

confidence: 99%

Magnetic nanoparticles: material engineering and emerging applications in lithography and biomedicine

et al. 2015

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We present an interdisciplinary overview of material engineering and emerging applications of iron oxide nanoparticles. We discuss material engineering of nanoparticles in the broadest sense, emphasizing size and shape control, large-area self-assembly, composite/hybrid structures, and surface engineering. This is followed by a discussion of several non-traditional, emerging applications of iron oxide nanoparticles, including nanoparticle lithography, magnetic particle imaging, magnetic guided drug delivery, and positive contrast agents for magnetic resonance imaging. We conclude with a succinct discussion of the pharmacokinetics pathways of iron oxide nanoparticles in the human body –– an important and required practical consideration for any in vivo biomedical application, followed by a brief outlook of the field.

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“…In addition, biotin is a natural biological molecule, causing concerns about the specificity and background when performing assays involving biotin-rich tissues and extracts (e.g., brain, liver, milk, or eggs). 28 …”

Section: Introductionmentioning

confidence: 99%

Linker-free conjugation and specific cell targeting of antibody functionalized iron-oxide nanoparticles

Baiu

Sherwood

et al. 2014

J. Mater. Chem. B

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Specific targeting is a key step to realize the full potential of iron oxide nanoparticles in biomedical applications, especially tumor-associated diagnosis and therapy. Here, we developed anti-GD2 antibody conjugated iron oxide nanoparticles for highly efficient neuroblastoma cell targeting. The antibody conjugation was achieved through an easy, linker-free method based on catechol reactions. The targeting efficiency and specificity of the antibody-conjugated nanoparticles to GD2-positive neuroblastoma cells were confirmed by flow cytometry, fluorescence microscopy, Prussian blue staining and transmission electron microscopy. These detailed studies indicated that the receptor-recognition capability of the antibody was fully retained after conjugation and the conjugated nanoparticles quickly attached to GD2-positive cells within four hours. Interestingly, longer treatment (12 h) led the cell membrane-bound nanoparticles to be internalized into cytosol, either by directly penetrating the cell membrane or escaping from the endosomes. Last but importantly, the uniquely designed functional surfaces of the nanoparticles allow easy conjugation of other bioactive molecules.

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Make Conjugation Simple: A Facile Approach to Integrated Nanostructures

Cited by 48 publications

References 43 publications

The responses of immune cells to iron oxide nanoparticles

The responses of immune cells to iron oxide nanoparticles

Magnetic nanoparticles: material engineering and emerging applications in lithography and biomedicine

Linker-free conjugation and specific cell targeting of antibody functionalized iron-oxide nanoparticles

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