Major Role of NAD-Dependent Lactate Dehydrogenases in Aerobic Lactate Utilization in
            <i>Lactobacillus plantarum</i>
            during Early Stationary Phase

Goffin, Philippe; Lorquet, Frédérique; Kleerebezem, Michiel; Hols, Pascal

doi:10.1128/jb.186.19.6661-6666.2004

Cited by 70 publications

(66 citation statements)

References 32 publications

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“…It has been reported that this gene plays an important role in production of L-lactate during fermentative growth of B. subtilis (14,34). In some other bacteria, similar NAD-dependent L-lactate dehydrogenases were shown to be capable of reversibly interconnecting pyruvate and lactate (23). We therefore wondered whether in B. subtilis, the lctE gene also contributes to L-lactate utilization.…”

Section: Resultsmentioning

confidence: 99%

A Widely Conserved Gene Cluster Required for Lactate Utilization inBacillus subtilisand Its Involvement in Biofilm Formation

2009

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We report that catabolism of L-lactate in Bacillus subtilis depends on the previously uncharacterized yvfVyvfW-yvbY (herein renamed lutABC) operon, which is inferred to encode three iron-sulfur-containing proteins. The operon is under the dual control of a GntR-type repressor (LutR, formerly YvfI) and the master regulator for biofilm formation SinR and is induced during growth in response to L-lactate. Operons with high similarity to lutABC are present in the genomes of a variety of gram-positive and gram-negative bacteria, raising the possibility that LutABC is a widely conserved and previously unrecognized pathway for the utilization of L-lactate or related metabolites.The spore-forming bacterium Bacillus subtilis is capable of forming complex multicellular communities on surfaces (8,25,30,40). These communities, known as biofilms, consist of long chains of cells that are held together by an extracellular matrix consisting of protein and polysaccharide (9,31,36,37). Production of the matrix is governed by a complex regulatory network, at the heart of which are two parallel pathways of repression and antirepression (4,(10)(11)(12)27). One pathway involves the repressor AbrB, which controls the expression of many different kinds of genes, including genes involved in biofilm formation, during the transition from exponential growth to stationary phase (4,12,24). Relief from AbrBmediated repression is brought about in part by the antirepressor AbbA, which binds to and inactivates the repressor (4). The other pathway, consisting of the repressor SinR and its antirepressor SinI, is dedicated largely to genes involved in biofilm formation (10)(11)(12)27). The principal targets of the SinR repressor are the 18 genes of the epsA-to-O and yqxM-sipW-tasA operons, which are responsible for the production of the matrix (7,11,27,32). Several other genes and operons that are not directly required for matrix production are also under the control of the SinI-SinR pathway (11), one of which, the yvfVyvfW-yvbY operon, is the subject of this report.The initial goal of the current investigation was to elucidate the role, if any, of the yvfV-yvfW-yvbY operon, whose protein products were of unknown function, in biofilm formation. As we report herein, a clue as to the function of the operon came from comparative genomics, which led to the discovery that the yvfV-yvfW-yvbY operon specifies a pathway for the utilization of L-lactate. Here we demonstrate that the operon is required for growth on L-lactate as a sole carbon source; that it is subject to dual regulation, which allows it to be induced during both growth in liquid culture and biofilm formation; and that the operon influences the architectural complexity of biofilms formed in the presence of L-lactate. We therefore rename yvfV, yvfW, and yvbY as lutA, lutB, and lutC, respectively (for lactate utilization). Interestingly, homologous operons of lutABC are found in the genomes of many different bacteria, including some only distantly related to B. subtilis. These observations sug...

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Section: Resultsmentioning

confidence: 99%

A Widely Conserved Gene Cluster Required for Lactate Utilization inBacillus subtilisand Its Involvement in Biofilm Formation

2009

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“…pGIBD001 is a derivative of pGICB002 (14), in which the luxAB genes have been replaced by a chloramphenicol resistance cassette (P32-cat). To obtain plasmid pGIBD001, plasmid pGICB002 was reverse amplified by PCR with primers pJUDdelcomX1 and pJUDdelcomX2, which hybridize upstream and downstream of luxAB, respectively, and then ligated to the P32-cat cassette obtained as a PvuII restriction fragment from pUC18Cm (19).…”

Section: Methodsmentioning

confidence: 99%

Adaptor Protein MecA Is a Negative Regulator of the Expression of Late Competence Genes in Streptococcus thermophilus

et al. 2012

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In Streptococcus thermophilus, the ComRS regulatory system governs the transcriptional level of comX expression and, hence, controls the early stage of competence development. The present work focuses on the posttranslational control of the activity of the sigma factor ComX and, therefore, on the late stage of competence regulation. In silico analysis performed on the S. thermophilus genome revealed the presence of a homolog of mecA (mecA St ), which codes for the adaptor protein that is involved in ComK degradation by ClpCP in Bacillus subtilis. Using reporter strains and microarray experiments, we showed that MecA St represses late competence genes without affecting the early competence stage under conditions that are not permissive for competence development. In addition, this repression mechanism was found not only to act downstream of comX expression but also to be fully dependent on the presence of a functional comX gene. This negative control was similarly released in strains deleted for clpC, mecA, and clpC-mecA. Under artificial conditions of comX expression, we next showed that the abundance of ComX is higher in the absence of MecA or ClpC. Finally, results of bacterial two-hybrid assays strongly suggested that MecA interacts with both ComX and ClpC. Based on these results, we proposed that ClpC and MecA act together in the same regulatory circuit to control the abundance of ComX in S. thermophilus.

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“…any bacteria can use lactate as the sole carbon and energy source for growth (9,16,17,22,30). NAD-independent lactate dehydrogenases (iLDHs), which catalyze the oxidation of lactate to pyruvate via a flavin-dependent mechanism, play an essential role in the utilization of lactate in most lactate-utilizing bacteria (8,15,16,17).…”

mentioning

confidence: 99%

“…NAD-independent lactate dehydrogenases (iLDHs), which catalyze the oxidation of lactate to pyruvate via a flavin-dependent mechanism, play an essential role in the utilization of lactate in most lactate-utilizing bacteria (8,15,16,17). iLDHs can be classified into 2 subfamilies (D-iLDH and L-iLDH) depending on their substrate specificities (D-lactate versus L-lactate) (14).…”

mentioning

confidence: 99%

Lactate Utilization Is Regulated by the FadR-Type Regulator LldR in Pseudomonas aeruginosa

Gao¹,

Hu²,

Zheng³

et al. 2012

Journal of Bacteriology

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Major Role of NAD-Dependent Lactate Dehydrogenases in Aerobic Lactate Utilization in Lactobacillus plantarum during Early Stationary Phase

Cited by 70 publications

References 32 publications

A Widely Conserved Gene Cluster Required for Lactate Utilization inBacillus subtilisand Its Involvement in Biofilm Formation

A Widely Conserved Gene Cluster Required for Lactate Utilization inBacillus subtilisand Its Involvement in Biofilm Formation

Adaptor Protein MecA Is a Negative Regulator of the Expression of Late Competence Genes in Streptococcus thermophilus

Lactate Utilization Is Regulated by the FadR-Type Regulator LldR in Pseudomonas aeruginosa

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