Major Outer Membrane Protein Variants of Chlamydia trachomatis Are Associated with Severe Upper Genital Tract Infections and Histopathology in San Francisco

Dean, Deborah; Oudens, Elizabeth; Bolan, Gail; Padian, Nancy; Schachter, Julius

doi:10.1093/infdis/172.4.1013

Cited by 107 publications

(98 citation statements)

References 31 publications

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“…2c, d). The genome of C. muridarum Nigg has been sequenced (Read et al, 2000) so we further confirmed the identity of the CM972 by amplifying and sequencing a region of ompA, the gene that encodes MOMP, using the primer set BII and FII (Dean et al, 1995). The DNA sequence of the amplified fragment was identical to both the parental Nigg strain and the published sequence (data not shown).…”

Section: Effect Of Novobiocin Treatment On Replication Of Chlamydiaesupporting

confidence: 70%

A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture

2006

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A highly conserved cryptic plasmid is present in Chlamydia trachomatis yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of C. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives of C. muridarum strain Nigg were generated at high efficiencies (4-30 %). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.

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Section: Effect Of Novobiocin Treatment On Replication Of Chlamydiaesupporting

confidence: 70%

A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture

2006

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“…The predominant Chlamydia trachomatis strains that are responsible for sexually transmitted diseases (STD) 1 worldwide are serovars E, F, and D (1)(2)(3)(4)(5). The public health costs for the morbidity and sequelae of these infections have reached $2.4 billion per year (6).…”

Section: Introductionmentioning

confidence: 99%

“…This research has revealed an array of genotypic variation within each of the known serovars (1,2,(11)(12)(13)(14)(15)(16)(17). Many of these variants contain amino acid changes which cannot be identified by serotyping (18,19).…”

Section: Introductionmentioning

confidence: 99%

Molecular and mutation trends analyses of omp1 alleles for serovar E of Chlamydia trachomatis. Implications for the immunopathogenesis of disease.

Dean¹,

Millman²

1997

J. Clin. Invest.

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Serovars E, F, and D are the most prevalent Chlamydia trachomatis strains worldwide. This prevalence may relate to epitopes that enhance infectivity and transmission. There are numerous major outer membrane protein (MOMP) gene ( omp1 ) variants described for D and F but few for E. However, omp1 constant regions are rarely sequenced, yet, they may contain mutations that affect the structure/function relationship of the protein. Further, differentiating variants that occur as a result of selection from variants that contain random mutations without biologic impact is difficult. We investigated 67 urogenital E serovars and found 11 (16%) variants which contained 16 (53%) nonconservative amino acid changes. Using signature-pattern analysis, 57 amino acids throughout MOMP differentiated the E sequence set from the non-E sequence set, thus defining E strains. Four E variants did not match this signature-pattern, and, by phenetic analyses, formed new phylogenetic branches, suggesting that they may be biologically distinct variants. Our analyses offer for the first time a unique approach for identifying variants that may occur from selection and may affect infectivity and transmission. Understanding the mutation trends, phylogeny, and molecular epidemiology of E variants is essential for designing public health control interventions and a vaccine. ( J. Clin. Invest. 1997. 99:475-483.)

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“…A role for spontaneous mutation followed by in vivo selection in the escape of C. trachomatis from human immune defenses is suggested by the high proportion of urogenital tract isolates that have amino acid substitutions in the polymorphic ompA gene, which encodes the major outer membrane protein (MOMP) (3,5,7,11,17,21,27). Humans have diverse B-cell-mediated (33) and T-cell-mediated (16,17,27,28) responses to MOMP.…”

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confidence: 99%

Interstrain Gene Transfer inChlamydia trachomatisIn Vitro: Mechanism and Significance

DeMars

Weinfurter

2008

J Bacteriol

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The high frequency of between-strain genetic recombinants of Chlamydia trachomatis among isolates obtained from human sexually transmitted infections suggests that lateral gene transfer (LGT) is an important means by which C. trachomatis generates variants that have enhanced relative fitness. A mechanism for LGT in C. trachomatis has not been described, and investigation of this phenomenon by experimentation has been hampered by the obligate intracellular development of this pathogen. We describe here experiments that readily detected LGT between strains of C. trachomatis in vitro. Host cells were simultaneously infected with an ofloxacin-resistant (Ofx r ) mutant of a serovar L1 strain (L1:Ofx r -1) and a rifampin-resistant (Rif r ) mutant of a serovar D strain (D:Rif r -1). Development occurred in the absence of antibiotics, and the progeny were subjected to selection for Ofx r Rif r recombinants. The parental strains differed at many polymorphic nucleotide sites, and DNA sequencing was used to map genetic crossovers and to determine the parental sources of DNA segments in 14 recombinants. Depending on the assumed DNA donor, the estimated minimal length of the transferred DNA was >123 kb in one recombinant but was >336 to >790 kb in all other recombinants. Such trans-DNA lengths have been associated only with conjugation in known microbial LGT systems, but natural DNA transformation remains a conceivable mechanism.LGT studies can now be performed with diverse combinations of C. trachomatis strains, and they could have evolutionary interest and yield useful recombinants for functional analysis of allelic differences between strains.

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Major Outer Membrane Protein Variants of Chlamydia trachomatis Are Associated with Severe Upper Genital Tract Infections and Histopathology in San Francisco

Cited by 107 publications

References 31 publications

A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture

A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture

Molecular and mutation trends analyses of omp1 alleles for serovar E of Chlamydia trachomatis. Implications for the immunopathogenesis of disease.

Interstrain Gene Transfer inChlamydia trachomatisIn Vitro: Mechanism and Significance

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