Major method-specific differences in the measurement of intact                parathyroid hormone: studies in patients with and without chronic renal                failure

Worth, Graeme K.; Vasikaran, Samuel; Retallack, R.W.; Musk, Alexander A.; Gutteridge, Donald H.

doi:10.1258/000456304322880050

Cited by 12 publications

(12 citation statements)

References 12 publications

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“…For both primary and renal HPT several intraoperative PTH assays have been described, but the different assays have only been directly compared in a small number of studies (13,18,22,(24)(25)(26)(27). The Immulite Turbo DPC assay is established for both primary HPT (24,28) and renal HPT (12).…”

Section: Discussionmentioning

confidence: 99%

“…Comparing both methods in terms of recovery of the C-terminal fragments of PTH (7-84), which are eliminated by glomerular filtration, Souberbielle et al (30) described a recovery of synthetic PTH (7-84) in 87.6% as measured with the ADVIA Centaur assay. For the Immulite assay, a recovery of PTH (7-84) was described in 62.7% by Worth et al (27). The different percentage of measuring PTH (7-84) with both assays showing a higher cross reactivity for the ADVIA Centaur assay might explain why this assay declined slower into the normal range in patients with impaired renal function compared with the Immulite assay.…”

Section: Discussionmentioning

confidence: 99%

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Clinical impact of two different intraoperative parathyroid hormone assays in primary and renal hyperparathyroidism

Meyer¹,

Zorn²,

Frank‐Raue³

et al. 2009

European Journal of Endocrinology

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Background: Intraoperative parathyroid hormone (PTH) monitoring predicts successful surgery for primary hyperparathyroidism (pHPT). In renal HPT, intraoperative PTH assays can define whether parathyroid resection is adequate. Methods: Intraoperative PTH was measured with two different immunometric assays (Immulite Turbo DPC and ADVIA Centaur assay) in 91 patients undergoing parathyroidectomy for primary (nZ57) and renal (nZ34) hyperparathyroidism. PTH was monitored preoperatively, 10, 20, and 30 min after parathyroidectomy and 24 h postoperatively. Results: Ten minutes after parathyroidectomy, intraoperative PTH dropped into the normal range (!7.6 pmol/l) in 84% of patients with pHPT and tertiary HPT as measured with the ADVIA Centaur assay (PTH-A), compared with 100% of the samples measured with the Immulite Turbo DPC assay (PTH-I; PZ0.0082). Twenty minutes after parathyroidectomy for secondary HPT, intraoperative PTH decreased to the normal range in 100% measured with PTH-I compared with 50% measured with PTH-A (PZ0.009). Then, 24 h postoperatively, PTH-I and PTH-A levels were within the normal range in all of the successfully treated patients. Both assays correctly identified six patients with persistent disease and another patient with a double adenoma in pHPT. Conclusions: In patients undergoing parathyroidectomy for primary or renal HPT, PTH levels decreasing to the normal range indicated successful surgery in all of the patients as measured with the PTH-I assay. Comparing the two assays, PTH-I was able to quantify the intraoperative PTH decay more quickly than PTH-A.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Clinical impact of two different intraoperative parathyroid hormone assays in primary and renal hyperparathyroidism

Meyer¹,

Zorn²,

Frank‐Raue³

et al. 2009

European Journal of Endocrinology

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“…Ces techniques sont calibrées contre de la PTH 1-84 synthé-tique d'origines variées, et il n'y a actuellement pas de standard international de PTH 1-84 synthétique. Si un tel standard devient disponible, il faudra intégrer dans les procédures de calibration l'interférence d'un possible effet matrice [41] ainsi que l'instabilité de la PTH synthétique lorsqu'elle est conservée pendant de longues périodes [42]. Enfin, si on montre de manière indiscutable qu'elles mesurent exactement les mêmes molécules, les techniques de troisième génération peuvent permettre de vraiment standardiser les résultats de PTH [43].…”

Section: Aspects Analytiques Et Préanalytiques Des Différentes Généraunclassified

Aspects pratiques des dosages de PTH

Souberbielle

Friedlander

Cormier

2006

Immuno-analyse & Biologie Spécialisée

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“…In the literature, there is only one reference dealing with the stability of PTH standards under different temperature conditions. 2 Our laboratory includes an aliquot of a human plasma pool 3 as an internal control. This pool is prepared from samples from different patients.…”

Section: Introductionmentioning

confidence: 99%

Effect of transport temperature on the Scantibodies total intact parathyroid hormone immunoradiometric assay using the RIAMAT-280

et al. 2010

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Background: Inadequate temperature affects the stability of intact parathyroid hormone (i-PTH) kits. Room temperature during transport modifies i-PTH results. Method: Percent bound (%B/Bmax) and concentrations ( pmol/L) of standards, controls (C1, C2) and pool from eight standard curves were divided into: group I (three curves from kits kept at room temperature for more than 48 h) and group II (five curves from kits kept at 2 -88C) during transport. i-PTH was measured using Scantibodies total i-PTH assay with RIAMAT-280. Results: %B/Bmax for standards, C1 and C2 were significantly higher in group I versus II (P ¼ 0.04). %B/Bmax for the pool were significantly lower in group I (P ¼ 0.001). i-PTH pool concentration in group I was 51% lower (95% confidence interval, 47-53%, P ¼ 0.001); differences were not significant for C1 (P ¼ 0.25) and C2 (P ¼ 0.57) in both groups. Conclusion: Room temperature on i-PTH kit during transport alters the standard curve, resulting in a decrease in i-PTH. Using a pool as internal quality control allows the detection of these changes not detected by kit controls.

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Major method-specific differences in the measurement of intact parathyroid hormone: studies in patients with and without chronic renal failure

Abstract: Background Following the introduction of two-site immunometric assays for parathyroid hormone (PTH), the expectation of good inter-assay agreement has not been fulfilled. The reasons for this may include differences in standardization as well as fragment recognition between the assays.

Cited by 12 publications

References 12 publications

Clinical impact of two different intraoperative parathyroid hormone assays in primary and renal hyperparathyroidism

Clinical impact of two different intraoperative parathyroid hormone assays in primary and renal hyperparathyroidism

Aspects pratiques des dosages de PTH

Effect of transport temperature on the Scantibodies total intact parathyroid hormone immunoradiometric assay using the RIAMAT-280

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